为研究人参皂苷对H2O2诱导的人结肠癌(Caco-2)细胞氧化应激损伤的保护作用,构建H2O2诱导的Caco-2细胞氧化损伤模型,以不同质量浓度(10、20、50 μg/mL)的人参皂苷干预H2O2诱导后的Caco-2细胞,通过形态学观察,CCK-8法检测细胞活力,测定胞内超氧化物歧化酶(superoxide dismutase,SOD)和谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)水平,测定氧化损伤标志物丙二醛(malondialdehyde,MDA)活力以及活性氧(reactive oxygen species,ROS)水平,评价人参皂苷的抗氧化应激水平。结果表明,200 μmol/L的H2O2诱导Caco-2细胞4 h后,细胞存活率达到58%左右,符合建模要求。添加10~50 μg/mL的人参皂苷明显提升受损细胞的存活率、SOD以及胞内GSH-Px活性,降低受损细胞内ROS和MDA水平,对H2O2诱导的Caco-2细胞氧化应激损伤起到良好的保护作用。
刘晓凤
,
卢晓琴
,
钟浩
,
HUSSAIN Muhammad
,
王丽娜
,
杨梦雨
,
张嘉宁
,
关荣发
. 人参皂苷对过氧化氢诱导的Caco-2细胞氧化损伤的保护作用[J]. 食品与发酵工业, 2024
, 50(7)
: 46
-50
.
DOI: 10.13995/j.cnki.11-1802/ts.035145
This study aimed to study the protective effect of ginsenosides on oxidative stress injury of human colon cancer (Caco-2) cells induced by hydrogen peroxide.Human colon cancer Caco-2 cells were cultured to construct the oxidative damage model of Caco-2 cells induced by hydrogen peroxide.The oxidative stress model of Caco-2 cells treated by ginsenosides at different concentrations (10, 20, and 50 μg/mL) was used to observe the cell vitality by morphological observation and the CCK-8 method.The intracellular superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) levels were determined.The activity of oxidative damage marker malondialdehyde (MDA) and reactive oxygen species (ROS) were measured to evaluate the antioxidant stress level of ginsenosides.Results showed that the survival rate of Caco-2 cells reached about 58% after inducing Caco-2 cells with 200 μmol/L H2O2 for 4 h, which met the modelling requirements.Ginsenosides (10-50 μg/mL) significantly increased the survival rate, SOD, and intracellular GSH-Px activity, and decreased the level of ROS and MDA in the damaged cells.Ginsenosides can protect Caco-2 cells from oxidative stress induced by hydrogen peroxide.
[1] 牛瑞娟, 马思佳, 唐亮, 等.人参皂苷Rh1免疫调节作用的研究进展[J].国际老年医学杂志, 2022, 43(3):368-371.
NIU R J, MA S J, TANG L, et al.Advances in immunomodulatory effects of ginsenoside Rh1[J].International Journal of Geriatrics, 2022, 43(3):368-371.
[2] JUNG D H, NAHAR J, MATHIYALAGAN R, et al.A focused review on molecular signalling mechanisms of ginsenosides anti-lung cancer and anti-inflammatory activities[J].Anti-Cancer Agents in Medicinal Chemistry, 2023, 23(1):3-14.
[3] REHMAN M U, ZHAO Q L, REFAAT A, et al.ROS associated ER-stress and intracellular calcium release contributes to CDDO-Me-mediated paraptosis in colorectal cancer cells[J].Free Radical Biology and Medicine, 2022, 180:s35.
[4] ZHOU Y M, XU B B, YU H Y, et al.Biochanin A attenuates ovariectomy-induced cognition deficit via antioxidant effects in female rats[J].Frontiers in Pharmacology, 2021, 12:603316.
[5] 林玥莹, 王敏奇.肠道微生物与肠上皮细胞互作关系的研究进展[J].中国畜牧杂志, 2021, 57(12):37-41;46.
LIN Y Y, WANG M Q.Research progress on interactions between intestinal microbiota and intestinal epithelial cells[J].Chinese Journal of Animal Science, 2021, 57(12):37-41;46.
[6] LIU C M, CHI K M, YANG M, et al.Staphylococcal enterotoxin A induces intestinal barrier dysfunction and activates NLRP3 inflammasome via NF-κB/MAPK signaling pathways in mice[J].Toxins, 2022, 14(1):29.
[7] KIMATU B M, FANG D L, ZHAO L Y, et al.Agaricus bisporus peptide fractions confer cytoprotective ability against hydrogen peroxide-induced oxidative stress in HepG2 and Caco-2 cells[J].Journal of Food Measurement and Characterization, 2020, 14(5):2503-2519.
[8] ZHANG L W, LI X L, ZHAO H B, et al.Influence of glutathione responsive tumor-targeted camptothecin nanoparticles on glioma based on oxidative stress[J].Colloid and Interface Science Communications, 2021, 42:100423.
[9] HOFFMANN P, BURMESTER M, LANGEHEINE M, et al.Caco-2/HT29-MTX co-cultured cells as a model for studying physiological properties and toxin-induced effects on intestinal cells[J].PLoS One, 2021, 16(10):e0257824.
[10] 刘聪秀,宋佳佳,王洪伟,等.发酵乳杆菌LFQ153胞外多糖对RAW264.7巨噬细胞氧化损伤的保护作用[J].食品与发酵工业, 2022, 48(8):1-8.
LIU C X, SONG J J, WANG H W, et al.Protective effect of exopolysaccharide from Lactobacillus fermentum LFQ153 against oxidative damage in RAW264.7 macrophages[J].Food and Fermentation Industries, 2022, 48(8):1-8.
[11] 何萍萍, 郑雅君, 郑明静, 等.红毛藻多糖对H2O2诱导的Caco-2细胞氧化损伤的保护作用[J].食品科学, 2021, 42(17):113-120.
HE P P, ZHENG Y J, ZHENG M J, et al.Protective effect of Bangia fusco-purpurea polysaccharide on H2O2-induced oxidative damage in Caco-2 cells[J].Food Science, 2021, 42(17):113-120.
[12] 吴金姗, 黄榕, 刘树英, 等.玉簪多糖对细胞氧化应激损伤的保护作用机制[J].食品科学, 2022, 43(17):138-146.
WU J S, HUANG R, LIU S Y, et al.Protective mechanism of polysaccharide from Hosta ventricosa against oxidative damage in cells[J].Food Science, 2022, 43(17):138-146.
[13] BUCKLEY S, BYRNES S, COCHRANE C, et al.The role of oxidative stress in HIV-associated neurocognitive disorders[J].Brain, Behavior, & Immunity-Health, 2021, 13:100235.
[14] VINU RAJAN P K.Activation of the oxidative stress in culex quinquefasciatus by the augmented production of reactive oxygen species (ROS) in response to Stachytarpheta jamaicensis exposure[J].Journal of Communicable Diseases, 2021, 53(2):43-51.
[15] 宁晨青. 人参皂苷Rg1的肝保护作用及其分子药理学机制[D].大连:大连医科大学, 2019.
NING C Q.Liver protective effects and molecular pharmacological mechanisms of ginsenoside Rg1[D].Dalian:Dalian Medical University, 2019.
[16] ZHENG Q H, TAN W J, FENG X L, et al.Protective effect of flavonoids from mulberry leaf on AAPH-induced oxidative damage in sheep erythrocytes[J].Molecules, 2022, 27(21):7625.
[17] ESMAEILNEJAD B, TAVASSOLI M, SAMIEI A, et al.Evaluation of oxidative stress and antioxidant status, serum trace mineral levels and cholinesterases activity in cattle infected with Anaplasma marginale[J].Microbial Pathogenesis, 2018, 123:402-409.
