综述与专题评论

海参多糖的提取纯化、化学分析方法和生物活性研究进展

  • 马向前 ,
  • 夏苏东 ,
  • 王苹 ,
  • 黄天卓 ,
  • 高哲颖 ,
  • 李茂哲
展开
  • 1(天津农学院 水产学院,天津市水产生态及养殖重点实验室,天津,300384)
    2(天津现代创新中药科技有限公司,天津,300380)
    3(天津市滨海新区农业农村发展服务中心,天津,300450)
第一作者:硕士研究生(夏苏东副研究员为通信作者,E-mail:xsd20022003@126.com)

收稿日期: 2023-02-28

  修回日期: 2023-05-05

  网络出版日期: 2024-05-09

基金资助

天津市教委科研计划项目(2022ZD004)

Research progress in extraction, purification, chemical analysis, and biological activity of sea cucumber polysaccharides

  • MA Xiangqian ,
  • XIA Sudong ,
  • WANG Ping ,
  • HUANG Tianzhuo ,
  • GAO Zheying ,
  • LI Maozhe
Expand
  • 1(Tianjin Key Laboratory of Aquatic Ecology and Aquaculture, School of Fisheries, Tianjin Agricultural University,Tianjin 300384, China)
    2(Tianjin Modern Innovation Traditional Chinese Medicine Technology Co.Ltd., Tianjin 300380, China)
    3(Tianjin Binhai New Area Agricultural and Rural Development Service Center, Tianjin 300450, China)

Received date: 2023-02-28

  Revised date: 2023-05-05

  Online published: 2024-05-09

摘要

海参是我国传统保健食品,具有极高的保健及营养价值。海参多糖是海参的重要活性成分之一,具有免疫调节、抗肿瘤、抗凝血、抗炎、降血糖、降血脂、抗氧化等生物活性,在食品和医疗领域有着巨大的开发价值和应用潜力。海参的品种、多糖的提取和纯化方法的不同,均会使制备的海参多糖在产率、化学组成、化学结构和生物活性等方面存在一定差异。该文综述了近几年国内外关于海参多糖的提取、分离纯化、含量测定、化学组成分析的方法及其生物活性,为海参多糖的深入研究以及开发应用提供理论依据。

本文引用格式

马向前 , 夏苏东 , 王苹 , 黄天卓 , 高哲颖 , 李茂哲 . 海参多糖的提取纯化、化学分析方法和生物活性研究进展[J]. 食品与发酵工业, 2024 , 50(7) : 364 -373 . DOI: 10.13995/j.cnki.11-1802/ts.035296

Abstract

Sea cucumber is a traditional health food in China, with high health and nutritional value.Sea cucumber polysaccharide is one of the important active ingredients of sea cucumber.It has biological activities such as immune regulation, anti-tumor, anti-coagulation, anti-inflammatory, hypoglycemic, hypolipidemic, and antioxidant, and has great development value and the application potential in the field of food and medical treatment.Different varieties of sea cucumber, extraction, and purification methods of polysaccharides will lead to certain differences in yield, chemical composition, chemical structure, and biological activity of sea cucumber polysaccharides.The methods of extraction, separation, purification, content determination, chemical composition analysis, and biological activities of sea cucumber polysaccharides at home and abroad in recent years were reviewed, providing a theoretical basis for further research, development, and application of sea cucumber polysaccharides.

参考文献

[1] PANGESTUTI R, ARIFIN Z.Medicinal and health benefit effects of functional sea cucumbers[J].Journal of Traditional and Complementary Medicine, 2018, 8(3):341-351.
[2] 廖玉麟. 我国的海参[J].生物学通报, 2001, 36(9):1-3;47.
LIAO Y L.Sea cucumber in China[J].Bulletin of Biology, 2001, 36(9):1-3;47.
[3] 韩秋菊, 马宏飞.海参多糖的提取与纯化研究[J].安徽农业科学, 2012, 40(14):8071-8072;8074.
HAN Q J, MA H F.Extraction and purification of polysaccharides in sea cucumber[J].Journal of Anhui Agricultural Sciences, 2012, 40(14):8071-8072;8074.
[4] 冯骏. 海参多糖的分离纯化与化学组成的研究[D].厦门:集美大学, 2014.
FENG J.Study on isolation, purification and chemical component analysis of polysaccharides fromsea cucumber[D].Xiamen:Jimei University, 2014.
[5] LI C, NIU Q F, LI S J, et al.Fucoidan from sea cucumber Holothuria polii:Structural elucidation and stimulation of hematopoietic activity[J].International Journal of Biological Macromolecules, 2020, 154:1123-1131.
[6] GONG P X, WU Y C, LIU Y, et al.Structure and hypoglycemic effect of a neutral polysaccharide isolated from sea cucumber Stichopus japonicus[J].International Journal of Biological Macromolecules, 2022, 216:14-23.
[7] 韩慧敏, 谢安, 罗杰英.海参多糖提取工艺研究[J].湖南中医杂志, 2000, 16(1):56-57.
HAN H M, XIE A, LUO J Y.Study on extraction technology of polysaccharide from sea cucumber[J].Hunan Journal of Traditional Chinese Medicine, 2000, 16(1):56-57.
[8] 陈发河, 李真, 吴光斌.海地瓜多糖和多肽提取纯化工艺及抗氧化活性[J].集美大学学报(自然科学版), 2018, 23(2):105-118.
CHEN F H, LI Z, WU G B.Study on the extraction and antioxidant activities of polysaccharides and polypeptides from Acaudina molpadioides(semper)[J].Journal of Jimei University (Natural Science), 2018, 23(2):105-118.
[9] 徐逯, 叶立斌, 于平, 等.东海海参多糖酶解提取工艺优化[J].食品科学, 2010, 31(20):61-66.
XU L, YE L B, YU P, et al.Optimization of enzymatic polysaccharide extraction from Acaudina molpadioides semper by response surface methodology[J].Food Science, 2010, 31(20):61-66.
[10] MOU J J, LI Q, QI X H, et al.Structural comparison, antioxidant and anti-inflammatory properties of fucosylated chondroitin sulfate of three edible sea cucumbers[J].Carbohydrate Polymers, 2018, 185:41-47.
[11] 展学孔, 周海妹, 马小花, 等.海参多糖提取新工艺[J].中国实验方剂学杂志, 2011, 17(15):40-42.
ZHAN X K, ZHOU H M, MA X H, et al.New extraction technology of polysaccharide from sea cucumber[J].Chinese Journal of Experimental Traditional Medical Formulae, 2011, 17(15):40-42.
[12] LI X M, LUO L, CAI Y, et al.Structural elucidation and biological activity of a highly regular fucosylated glycosaminoglycan from the edible sea cucumber Stichopus herrmanni[J].Journal of Agricultural and Food Chemistry, 2017, 65(42):9315-9323.
[13] HE W T, SUN H H, SU L J, et al.Structure and anticoagulant activity of a sulfated fucan from the sea cucumber Acaudina leucoprocta[J].International Journal of Biological Macromolecules, 2020, 164:87-94.
[14] 王远红, 吕志华, 姜廷福, 等.梅花参中多糖提取工艺及含量测定的研究[J].中国海洋大学学报(自然科学版), 2005, 35(6):987-990.
WANG Y H, LV Z H, JIANG T F, et al.Extraction and determination of polysaccharides in the holothutia[J].Journal of Ocean University of Qingdao, 2005, 35(6):987-990.
[15] WANG J J, SHI S, LI F F, et al.Physicochemi-cal properties and antioxidant activity of polysaccharides obtained from sea cucumber gonads via ultrasound-assisted enzymatic techniques[J].LWT-Food Science and Technology, 2022, 160:113307.
[16] 王庆芬. 刺参中多糖的提取分离、结构表征及其核苷类物质的分析[D].福州:福建医科大学, 2017.
WANG Q F.Extraction, separation, structural characterization of polysaccharide and analysis on nucleoside in Stichopus japonicus[D].Fuzhou:Fujian Medical University, 2017.
[17] 王婧媛, 王联珠, 孙晓杰, 等.海参加工工艺、营养成分及活性物质研究进展[J].食品安全质量检测学报, 2018, 9(11):2749-2755.
WANG J Y, WANG L Z, SUN X J, et al.Research progress on processing technology, nutritive components and active substances of sea cucumber[J].Journal of Food Safety & Quality, 2018, 9(11):2749-2755.
[18] 于双, 李双双, 宋志远, 等.海参多糖提取 分离及纯化的研究概述[J].农产品加工, 2022(7):80-83;88.
YU S, LI S S, SONG Z Y, et al.Research on extraction separation purification methods of sea cucumber polysaccharides[J].Farm Products Processing, 2022(7):80-83;88.
[19] 汪国威. 海南玉足海参的化学成分研究[D].海口:海南大学, 2018.
WANG G W.Studies on the chemical constituents of sea cucumber in Yuzu, Hainan[D].Haikou:Hainan University, 2018.
[20] YUAN Q X, LI H, WANG Q, et al.Deaminative-cleaved S.monotuberculatus fucosylated glycosaminoglycan:Structural elucidation and anticoagulant activity[J].Carbohydrate Polymers, 2022, 298:120072.
[21] 宋思媛, 李美琪, 王泽旭, 等.海参多糖对小鼠肠道菌群紊乱的恢复作用[J].中国微生态学杂志, 2019, 31(9):1 023-1026; 1033.
SONG S Y, LI M Q, WANG Z X, et al. Restoration effect of sea cucumber polysaccharides on intestinal microbiota disorder in mice[J]. Chinese Journal of Microecology, 2019, 31(9):1023-1026; 1033.
[22] 巫若子. 紫米花青素稳定性及应用研究进展[J].印染助剂, 2022, 39(10):7-10.
WU R Z.Research progress on the stability and application of purple rice anthocyanin[J].Textile Auxiliaries, 2022, 39(10):7-10.
[23] 李哲斌. 芋头多糖提取纯化、结构表征及生物活性研究进展[J].食品与发酵工业, 2023, 49(24):354-360.
LI Z B.Research progress on extraction, purification, structure characterization, and biological activity of taro polysaccharides[J].Food and Fermentation Industries, 2023, 49(24):354-360.
[24] 张军瑞, 陈健.木瓜蛋白酶酶解白底辐肛参提取多糖的研究[J].现代食品科技, 2009, 25(5):542-545.
ZHANG J R, CHEN J.Papain-catalyzed hydrolysis of Actinopyga mauritiana for mucopolysaccharide preparation[J].Modern Food Science and Technology, 2009, 25(5):542-545.
[25] 宿玮, 王彦超, 张翠玉, 等.海地瓜多糖脱色工艺研究[J].离子交换与吸附, 2011, 27(6):521-529.
SU W, WANG Y C, ZHANG C Y, et al.Study on decolorization technology of polysaccharide from Acaudina molpadioides[J].Ion Exchange and Adsorption, 2011, 27(6):521-529.
[26] 钮婧杰, 孙延平, 李霄, 等.树脂在中药多糖脱色工艺中的研究进展[J].化学工程师, 2021, 35(1):48-51.
NIU J J, SUN Y P, LI X, et al.Research progress of microporous resin in decolorization of polysaccharides from traditional Chinese medicine[J].Chemical Engineer, 2021, 35(1):48-51.
[27] 韩伟, 黄兮, 张玲玲, 等.中药多糖的提取、分离纯化及分析方法的研究进展[J].机电信息, 2012(14):19-25.
HAN W, HUANG X, ZHANG L L, et al.Advances in the extraction, isolation, purification and assay methods of polysaccharides in Chinese traditional medicine[J].Mechanical and Electrical Information, 2012(14):19-25.
[28] 朱启源. 两种海参多糖组分的制备及其改善大鼠2型糖尿病作用研究[D].广州:华南理工大学, 2020.
ZHU Q Y.Preparation of two sea cucumber polysaccharide fractions and their ameliorative effects on type 2 diabetes in rats[D].Guangzhou:South China University of Technology, 2020.
[29] 高岳. 不同产地刺参多糖的分离纯化及其组分含量的研究[D].大连:大连海洋大学, 2015.
GAO Y.Purification of polysaccharides and analysis of their contents and compositions in sea cucumbers(Apostichopus japonicas) from four origins[D].Dalian:Dalian Ocean University, 2015.
[30] 朱俊友, 李志佳, 董赵微, 等.香菇多糖提取、结构和生物活性的研究进展[J].河南工业大学学报(自然科学版), 2022, 43(5):125-136.
ZHU J Y, LI Z J, DONG Z W, et al.Research progress of extraction, structure, and biological activity of lentinan[J].Journal of Henan University of Technology (Natural Science Edition), 2022, 43(5):125-136.
[31] CHANG Y G, HU Y F, YU L, et al.Primary structure and chain conformation of fucoidan extracted from sea cucumber Holothuria tubulosa[J].Carbohydrate Polymers, 2016, 136:1091-1097.
[32] GONG P X, LI Q Y, WU Y C, et al.Structural elucidation and antidiabetic activity of fucosylated chondroitin sulfate from sea cucumber Stichopus japonicas[J].Carbohydrate Polymers, 2021, 262:117969.
[33] NIELSEN S S.Phenol-Sulfuric Acid Method for Total Carbohydrates[M]//NIELSEN SS.Food Analysis Laboratory Manual.Boston, MA:Springer, 2010:47-53.
[34] 罗素菜, 张海江.王不留行药材中多糖含量测定研究[J].亚太传统医药, 2021, 17(11):47-50.
LUO S C, ZHANG H J.Study on the determination of polysaccharide content in Semen vaccaria[J].Asia-Pacific Traditional Medicine, 2021, 17(11):47-50.
[35] 焦健, 朱振军, 佟志晏, 等.HPLC-MS/MS法分析刺参及其制品中海参多糖的含量[J].中国渔业质量与标准, 2020, 10(5):12-17.
JIAO J, ZHU Z J, TONG Z Y, et al.Quantification of sea cucumber polysaccharide in Apostichopus japonicus and its relevant products[J].Chinese Fishery Quality and Standards, 2020, 10(5):12-17.
[36] 刘芬, 孙晓杰, 朱文嘉, 等.海参中单糖检测方法的建立及含量测定[J].渔业科学进展, 2018, 39(2):177-182.
LIU F, SUN X J, ZHU W J, et al.Establishment and determination of monosaccharide in sea cucumber[J].Progress in Fishery Sciences, 2018, 39(2):177-182.
[37] 顾兴宇, 白光辉, 刘璐, 等.海参多糖结构表征的研究进展[J].中国海洋药物, 2023, 42(1):66-75.
GU X Y, BAI G H, LIU L, et al.Research progress on structural characterization of sea cucumber polysaccharide[J].Chinese Journal of Marine Drugs, 2023, 42(1):66-75.
[38] ZHENG W Q, ZHOU L T, LIN L S, et al.Physicochemical characteristics and anticoagulant activities of the polysaccharides from sea cucumber Pattalus mollis[J].Marine Drugs, 2019, 17(4):198.
[39] LUO L, WU M Y, XU L, et al.Comparison of physicochemical characteristics and anticoagulant activities of polysaccharides from three sea cucumbers[J].Marine Drugs, 2013, 11(12):399-417.
[40] 杨东达.海参内脏多糖的分离、结构鉴定、免疫活性及其应用研究[D].泉州:华侨大学, 2020.
YANG D D.Study on isolation, structure identification, immune activity and application of polysaccharides from sea cucumber viscera[D].Quanzhou:Huaqiao University, 2020.
[41] 王婷, 刘京熙, 张健, 等.海参精多糖提取工艺优化及其体外抗肿瘤活性[J].食品工业科技, 2016, 37(17):68-74.
WANG T, LIU J X, ZHANG J, et al.Optimization of extraction of sea cucumber sperm polysaccharide and its antitumor activity in vitro[J].Science and Technology of Food Industry, 2016, 37(17):68-74.
[42] 卢战辉, 白阳秋, 孙趁意, 等.海参多糖调控JAK2/STAT3/survivin通路对肝癌细胞增殖和凋亡的影响[J].中国普外基础与临床杂志, 2022, 29(7):875-880.
LU Z H, BAI Y Q, SUN C Y, et al.Effects of sea cucumber polysaccharide regulating JAK2/STAT3/survivin pathway on proliferation and apoptosis of hepatocellular carcinoma cells[J].Chinese Journal of Bases and Clinics in General Surgery, 2022, 29(7):875-880.
[43] 李天, 刘一帆, 周东梅, 等.海参多糖抑制人肾癌细胞A498的生长转移作用机制[J].基因组学与应用生物学, 2020, 39(3):1344-1350.
LI T, LIU Y F, ZHOU D M, et al.Mechanism of the effect of sea cucumber polysaccharide on inhibiting the growth and metastasis of human RCC A498 cells[J].Genomics and Applied Biology, 2020, 39(3):1344-1350.
[44] 张晓媛. 海参多糖联合放疗对人肺腺癌A549细胞系的增殖抑制及促凋亡作用研究[D].青岛:青岛大学, 2019.
ZHANG X Y.Combination of holothurian glycosaminoglycan and radiation has synergistically inhibitory effects on human lung adenocarcinoma A549[D].Qingdao:Qingdao University, 2019.
[45] 王静杰, 钟强, 董春晖, 等.海参多糖生物学活性及其作用机制研究进展[J].食品科学, 2021, 42(23):370-380.
WANG J J, ZHONG Q, DONG C H, et al.Recent progress in the biological activity and mechanism of action of sea cucumber polysaccharides[J].Food Science, 2021, 42(23):370-380.
[46] GAO N, CHEN R, MOU R R, et al.Purification, structural characterization and anticoagulant activities of four sulfated polysaccharides from sea cucumber Holothuria fuscopunctata[J].International Journal of Biological Macromolecules, 2020, 164:3421-3428.
[47] NING Z M, WANG P, ZUO Z C, et al.A Fucan sulfate with pentasaccharide repeating units from the sea cucumber Holothuria floridana and its anticoagulant activity[J].Marine Drugs, 2022, 20(6):377.
[48] 张哲娴. 玉足海参(Holothuria leucospilota)多糖的化学结构与抗凝活性研究[D].武汉:中南民族大学, 2021.
ZHANG Z X.Studies on the chemical structure and anticoagulant activity of polysaccharides from Holothuria leucospilota[D].Wuhan:Central South University for Nationalities, 2021.
[49] ZHU Y J, TIAN Y Y, WANG N, et al.Structure-function relationship analysis of fucoidan from sea cucumber (Holothuria tubulosa) on ameliorating metabolic inflammation[J].Journal of Food Biochemistry, 2021, 45(1):e13500.
[50] 张帅. 刺参岩藻聚糖硫酸酯对RAW264.7细胞炎症和自噬的影响[D].杨凌:西北农林科技大学, 2021.
ZHANG S.Effects of fucoidan from Stichopus japonicus on inflammation and autophagy of RAW264.7 cells[D].Yangling:Northwest A & F University, 2021.
[51] 袁义琼. 玉足海参多糖对大鼠高脂血症的缓解作用及机制研究[D].海口:海南大学, 2020.
YUAN Y Q.Study on the alleviative effect and mechanism of polysaccharides from sea cucumber of Yuzu on hyperlipidemia in rats[D].Haikou:Hainan University, 2020.
[52] 李启冬. 海参肠壁硫酸软骨素的结构解析和活性研究[D].哈尔滨:哈尔滨工业大学, 2021.
LI Q D.Structure analysis and activity study of chondroitin sulfate from intestinal wall of sea cucumber[D].Harbin:Harbin Institute of Technology, 2021.
[53] GAO L, XU C, TAO X L, et al.Structure elucidation of Fucan sulfate from sea cucumber Holothuria fuscopunctata through a bottom-up strategy and the antioxidant activity analysis[J].International Journal of Molecular Sciences, 2022, 23(9):4488.
[54] WANG W L, MAO H, LI S J, et al.Branched chondroitin sulfate oligosaccharides derived from the sea cucumber Acaudina molpadioides stimulate neurite outgrowth[J].Marine Drugs, 2022, 20(10):653.
[55] 冯巧巧, 谢纪珍, 樊红延, 等.低分子量海参多糖胶囊对小鼠酒精性肝损伤的保护作用[J].卫生研究, 2018, 47(1):146-147.
FENG Q Q, XIE J Z, FAN H Y, et al.Protective effect of low molecular weight sea cucumber polysaccharide capsule on alcoholic liver injury in mice[J].Journal of Hygiene Research, 2018, 47(1):146-147.
[56] LI G, ZHOU Y, YANG W Y, et al.Inhibitory effects of sulfated polysaccharides from the sea cucumber Cucumaria frondosa against Aβ40 aggregation and cytotoxicity[J].ACS Chemical Neuroscience, 2021, 12(11):1854-1859.
[57] ZHU Z J, ZHU B W, SUN Y J, et al.Sulfated polysaccharide from sea cucumber and its depolymerized derivative prevent obesity in association with modification of gut microbiota in high-fat diet-fed mice[J].Molecular Nutrition & Food Research, 2018, 62(23):e1800446.
[58] LI J H, LI S, YAN L F, et al.Fucosylated chondroitin sulfate oligosaccharides exert anticoagulant activity by targeting at intrinsic tenase complex with low FXII activation:Importance of sulfation pattern and molecular size[J].European Journal of Medicinal Chemistry, 2017, 139:191-200.
[59] WU N, YE X Q, GUO X, et al.Depolymerization of fucosylated chondroitin sulfate from sea cucumber, Pearsonothuria graeffei, via 60Co irradiation[J].Carbohydrate Polymers, 2013, 93(2):604-614.
[60] CHEN S G, LI G Y, WU N, et al.Sulfation pattern of the fucose branch is important for the anticoagulant and antithrombotic activities of fucosylated chondroitin sulfates[J].Biochimica et Biophysica Acta (BBA)-General Subjects, 2013, 1830(4):3054-3066.
文章导航

/