This study aimed to study the uric acid (UA) lowering activity of Sanghuangporus sanghuang ethanol extract in vitro, the inhibitory activity of free phenol extracts of four different sources of Sanghuangporus sanghuang (wild Phellinus vaninii, cultivated Inonotus hispidus, cultivated Phellinus vaninii, and wild Inonotus hispidus) on xanthine oxidase was determined.Furthermore, the inhibitory effect of free phenol extracts on uric acid production in high uric acid cell models.Additionally, the main compounds in strong active extract were analyzed by UPLC-MS/MS.Results suggested that cultivated Inonotus hispidus, cultivated Phellinus vaninii, and wild Inonotus hispidus showed strong inhibitory effects on xanthine oxidase (XOD), the IC50 values were (94.63±2.73) μg/mL, (99.69±2.50) μg/mL, and (106.32±5.06) μg/mL, respectively.Cultivated Inonotus hispidus and wild Phellinus vaninii showed better UA lowering ability than those of wild Inonotus hispidus and cultivated Phellinus vaninii in the high UA cell model (P<0.05).Results obtained from UPLC-MS/MS showed that the strong active components of flavonoids including hyperoside, (-)-epicatechin, theaflavin, diosmin, and cynaroside, may be the active components for lowering uric acid.This study may provide some theoretical references for the application of uric acid-lowering products of Sanghuangporus sanghuang.
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