研究报告

微酸性电解水处理对兔肉冷藏期间品质及微生物菌群的影响

  • 左红 ,
  • 吴周林 ,
  • 张佳敏 ,
  • 何薇 ,
  • 张弛 ,
  • 杨思艺 ,
  • 王卫
展开
  • (成都大学,肉类加工四川省重点实验室,四川 成都,610106)
第一作者:硕士研究生(张佳敏教授为通信作者,E-mall:cdusp@qq.com)

收稿日期: 2023-06-06

  修回日期: 2023-08-07

  网络出版日期: 2024-06-11

基金资助

国家现代农业产业技术体系建设专项资金(CARS-43);四川省科技成果转移转化示范项目(2022ZHCG0063);四川省科技计划重点研发项目(2023YFN0056)

Effects of slightly acidic electrolytic water treatment on quality and microbiological community structure of rabbit meat during refrigerated storage

  • ZUO Hong ,
  • WU Zhoulin ,
  • ZHANG Jiamin ,
  • HE Wei ,
  • ZHANG Chi ,
  • YANG Siyi ,
  • WANG Wei
Expand
  • (Meat Processing Key Laboratory of Sichuan Province, Chengdu University, Chengdu 610106, China)

Received date: 2023-06-06

  Revised date: 2023-08-07

  Online published: 2024-06-11

摘要

为探究微酸性电解水(slightly acidic electrolytic water,SAEW)对兔肉贮藏品质的影响,将新鲜兔肉分别使用SAEW浸泡处理5 min(SWa)、SAEW浸泡处理10 min(SWb)、无菌水浸泡处理5 min(PW)和未经浸泡处理为对照组(CK)。处理后样品使用托盘覆膜,置于4 ℃冷藏。每2 d检测样品的总挥发性盐基氮(total volatile base nitrogen,TVB-N)含量、硫代巴比妥酸(thiobarbituric acid,TBA)含量、pH值、色度、感官及微生物菌群结构的组成及变化。结果表明,SAEW浸泡处理兔肉在冷藏期间pH值、TVB-N值、TBA值上升速率明显低于CK组和PW组,且能够更好地保持兔肉原有色泽以及感官品质,当贮藏到第8天时,发现TVB-N值在CK组[(14.98±0.42) mg/100 g]和PW组[(14.76±0.40) mg/100 g]显著高于SWa组[(13.58±0.40) mg/100 g]和SWb组[(12.66±0.43) mg/100 g]。结合16S rDNA测序分析,SAEW可抑制兔肉冷藏期间腐败菌拟杆菌属(Bacteroides)、假单胞菌属(Pseudzaomonas)的生长繁殖,有效延缓冷藏兔肉腐败变质且SAEW处理10 min减菌效果更佳。该研究结果为SAEW在兔肉贮藏保鲜中的应用提供了理论参考。

本文引用格式

左红 , 吴周林 , 张佳敏 , 何薇 , 张弛 , 杨思艺 , 王卫 . 微酸性电解水处理对兔肉冷藏期间品质及微生物菌群的影响[J]. 食品与发酵工业, 2024 , 50(10) : 127 -133 . DOI: 10.13995/j.cnki.11-1802/ts.036381

Abstract

This study aimed to investigate the effects of slightly acidic electrolytic water (SAEW) on the quality of rabbit meat during refrigerated storage.Fresh rabbit meat was soaked in SAEW for 5 min (SWa), SAEW for 10 min (SWb), pure water for 5 min (PW) and without soaking as control group (CK).All samples were coated with a tray and refrigerated at 4 ℃.These samples were collected every 2 days and tested for total volatile base nitrogen (TVB-N) content, thiobarbituric acid (TBA) content, pH value, chroma, sensory and microbial flora structure composition and change.Results showed that the rate of increase in pH, TVB-N and TBA values of SAEW-treated rabbit meat during refrigeration was significantly lower than that of the CK and PW groups, and the original colour and organoleptic qualities of rabbit meat could be better maintained.When stored up to the 8th day, the value of TVB-N in the CK group[(14.98±0.42) mg/100 g] and PW group[(14.76±0.40) mg/100 g] was significantly higher than that in the SWa group[(13.58±0.40) mg/100 g] and SWb group[(12.66±0.43) mg/100 g].Combined with 16S rDNA sequencing analysis, SAEW could inhibit the growth and reproduction of the spoilage bacteria Bacteroides and Pseudomonas during the period of refrigerated rabbit meat.This treatment could effectively delay the spoilage of refrigerated rabbit meat, and the effect of SAEW treatment for 10 min was better.The results of this study provide a theoretical reference for the application of SAEW in the storage and preservation of rabbit meat.

参考文献

[1] 张钰麟, 陈泓帆, 赵志平, 等.不同保鲜方式冷吃兔中挥发性风味物质的对比分析[J].中国调味品, 2022, 47(8):28-34;56.
ZHANG Y L, CHEN H F, ZHAO Z P, et al.Comparative analysis of volatile flavor substances in spicy rabbit meat rabbits with different preservation methods[J].China Condiment, 2022, 47(8):28-34;56.
[2] 戚铭, 罗自生, 王蕾, 等.微酸性电解水在食品保鲜消毒领域的应用[J].食品安全质量检测学报, 2020, 11(12):3795-3802.
QI M, LUO Z S, WANG L, et al.Application of slightly acidic electrolyzed water in food preservation and disinfection[J].Journal of Food Safety & Quality, 2020, 11 (12):3795-3802.
[3] 金素莱曼, 陈百科, 李慧, 等.酸性电解水预处理结合不同冰藏方式对大黄鱼保鲜效果的影响[J].食品与发酵工业, 2022, 48(8):128-135.
JIN S L M, CHEN B K, LI H, et al.Effects of acidic electrolyzed water pretreatment combined with different ice preservation on the quality of large yellow croaker (Pseudosciaena crocea)[J].Food and Fermentation Industries, 2022, 48(8):128-135.
[4] SUN J Z, JIANG X J, CHEN Y H, et al.Recent trends and applications of electrolyzed oxidizing water in fresh foodstuff preservation and safety control[J].Food Chemistry, 2022, 369:130873.
[5] DING T, XUAN X T, LI J, et al.Disinfection efficacy and mechanism of slightly acidic electrolyzed water on Staphylococcus aureus in pure culture[J].Food Control, 2016, 60:505-510.
[6] 曹悦, 刘泷泽, 柳红莉, 等.超声联合弱酸性电解水处理对草鱼鱼片冷藏期间品质及菌群的影响[J].食品安全质量检测学报, 2022, 13(8):2465-2473.
CAO Y, LIU L Z, LIU H L, et al.Effects of ultrasonic combined with slightly acidic electrolyzed water treatment on quality and bacterial flora of Ctenopharyngodon idella fillets during refrigerated storage[J].Journal of Food Safety & Quality, 2022, 13(8):2465-2473.
[7] 林依乐, 杜涓, 韩千慧, 等.基于高通量测序分析加工处理与贮藏时间对生鲜鸭肉中菌群结构及多样性的影响[J].食品科技,2022,47(12):313-321.
LIN Y L, DU J, HAN Q H, et al. Bacterial communities structure and diversity in chilled duck meat during processing and cold storage based on high-throughput sequencing technology. Food Science and Technology, 2022, 47(12):313-321.
[8] DUAN J Y, JIANG Y, CHERIAN G, et al.Effect of combined chitosan-krill oil coating and modified atmosphere packaging on the storability of cold-stored lingcod (Ophiodon elongates) fillets[J].Food Chemistry, 2010, 122(4):1035-1042.
[9] BING S, ZANG Y T, LI Y J, et al.A combined approach using slightly acidic electrolyzed water and tea polyphenols to inhibit lipid oxidation and ensure microbiological safety during beef preservation[J].Meat Science, 2022, 183:108643.
[10] SHENG X W, SHU D Q, TANG X J, et al.Effects of slightly acidic electrolyzed water on the microbial quality and shelf life extension of beef during refrigeration[J].Food Science & Nutrition, 2018, 6(7):1 975-1 981.
[11] 吴怡, 蓝蔚青, 刘嘉莉, 等.微酸性电解水结合迷迭香提取物对冷藏鲈鱼片品质变化的影响[J].食品与发酵工业, 2022, 48(4):47-55.
WU Y, LAN W Q, LIU J L, et al.Effects of slightly acidic electrolyzed water combined rosemary extract on the quality change of sea bass fillets (Lateolabrax japonicas) during refrigerated storage[J].Food and Fermentation Industries, 2022, 48(4):47-55.
[12] LI T T, LI J R, HU W Z.Changes in microbiological, physicochemical and muscle proteins of post mortem large yellow croaker (Pseudosciaena crocea)[J].Food Control, 2013, 34(2):514-520.
[13] HUANG Z, LIU X C, JIA S L, et al.The effect of essential oils on microbial composition and quality of grass carp (Ctenopharyngodon idellus) fillets during chilled storage[J].International Journal of Food Microbiology, 2018, 266:52-59.
[14] 张溪, 蓝蔚青, 迪丽菲拉·库尔班, 等.不同减菌预处理对鲜南美白对虾虾仁冷藏期间品质变化的影响[J].食品与发酵工业, 2020, 46(12):158-165.
ZHANG X,LAN W Q, DILIFEILA·KUERBAN, et al.Effects of different sterilization pretreatments on the quality of freshly peeled shrimps(Litopenaeus vannamei) during refrigerated storage[J].Food and Fermentation Industries, 2020, 46(12):158-165.
[15] 蓝蔚青, 张炳杰, 周大鹏, 等.超声联合微酸性电解水处理对真空包装海鲈鱼冷藏期间品质变化的影响[J].食品科学, 2022, 43(5):62-68.
LAN W Q, ZHANG B J, ZHOU D P, et al.Effect of ultrasonic combined with slightly acidic electrolyzed water treatment on quality changes of vacuum-packaged sea bass(Lateolabrax japonicas) during refrigerated storage[J].Food Science, 2022, 43(5):62-68.
[16] RAHMAN S M E, PARK J, SONG K B, et al.Effects of slightly acidic low concentration electrolyzed water on microbiological, physicochemical, and sensory quality of fresh chicken breast meat[J].Journal of Food Science, 2012, 77(1):M35-M41.
[17] 吴雨轩, 何瀚文, 张毅, 等.南美白对虾在酸性电解水减菌处理下鲜度品质及挥发性风味物质的变化[J].现代食品科技, 2022, 38(1):296-305.
WU Y X, HE H W, ZHANG Y, et al.Changes of freshness quality and volatile flavor compounds of Pacific white shrimp(Penaeus vannameri) under acidic electrolytic water treatment[J].Modern Food Science and Technology, 2022, 38(1):296-305.
[18] HUI G H, LIU W, FENG H L, et al.Effects of chitosan combined with nisin treatment on storage quality of large yellow croaker (Pseudosciaena crocea )[J].Food Chemistry, 2016, 203:276-282.
[19] 赵德锟, 高薇珊, 谭雷姝, 等.微酸性电解水对宣威火腿切片杀菌效果的研究[J].食品安全质量检测学报, 2016, 7(10):4059-4065.
ZHAO D K, GAO W S, TAN L S, et al.Sterilization effects of sliced Xuanwei ham treated with slightly acidic electrolyzed water[J].Journal of Food Safety & Quality, 2016, 7 (10):4059-4065.
[20] RODRÍGUEZ-CALLEJA J M, GARCÍA-LÓPEZ M L, SANTOS J A, et al.Development of the aerobic spoilage flora of chilled rabbit meat[J].Meat Science, 2005, 70(2):389-394.
[21] 于福田. 微酸性电解水对罗非鱼片杀菌和保鲜效果的研究[D].上海:上海海洋大学, 2019.
YU F T. Study on Sterilization and preservation of tilapia fillet using slightly acidic electrolyzed water. Shanghai: Shanghai Ocean University, 2019.
[22] NIKOLAEV Y, YUSHINA Y, MARDANOV A, et al.Microbial biofilms at meat-processing plant as possible places of bacteria survival[J].Microorganisms, 2022, 10(8):1583.
[23] 李宁. 冷鲜猪肉中微生物多样性和优势腐败菌分子检测技术研究[D].无锡:江南大学, 2019.
LI N.Study on microbial diversity and molecular detection technology for dominant spoilage bacteria in chilled pork[D].Wuxi:Jiangnan University, 2019.
[24] XUAN X T, DING T, LI J, et al.Estimation of growth parameters of Listeria monocytogenes after sublethal heat and slightly acidic electrolyzed water (SAEW) treatment[J].Food Control, 2017, 71:17-25.
[25] ERCOLINI D, CASABURI A, NASI A, et al.Different molecular types of Pseudomonas fragi have the same overall behaviour as meat spoilers[J].International Journal of Food Microbiology, 2010, 142(1-2):120-131.
[26] SALAZAR J K, WANG Y, YU S J, et al.Polymerase chain reaction-based serotyping of pathogenic bacteria in food[J].Journal of Microbiological Methods, 2015, 110:18-26.
[27] LI F F, ZHONG Q, KONG B H, et al.Synergistic effect and disinfection mechanism of combined treatment with ultrasound and slightly acidic electrolyzed water and associated preservation of mirror carp (Cyprinus carpio L.) during refrigeration storage[J].Food Chemistry, 2022, 386:132858.
[28] OKANDA T, TAKAHASHI R, EHARA T, et al.Slightly acidic electrolyzed water disrupts biofilms and effectively disinfects Pseudomonas aeruginosa[J].Journal of Infection and Chemotherapy: Official Journal of the Japan Society of Chemotherapy, 2019, 25(6):452-457.
[29] 叶章颖, 祁凡雨, 裴洛伟, 等.微酸性电解水对虾仁的杀菌效果及其动力学[J].农业工程学报, 2014, 30(3):223-230.
YE Z Y, QI F Y, PEI L W, et al.Disinfection effect and kinetics of slightly acidic electrolyzed water for white shrimp[J].Transactions of the Chinese Society of Agricultural Engineering, 2014, 30(3):223-230.
文章导航

/