三萜是药食用真菌樟芝野生子实体中主要的活性成分之一,但是人工皿式培养的樟芝菌丝体中三萜含量较低。为了提高樟芝皿式培养三萜的产量,该研究在培养基中添加了3种单萜作为刺激因子。通过添加浓度的优化,得到了对生物量具有显著促进作用的添加方式是0.08 g/L的α-松油醇;对三萜产率具有显著促进作用的最佳添加方式为0.16 g/L的松油烯、0.08 g/L的α-松油醇以及0.12 g/L的4-萜烯醇,三萜产率分别较对照组提高了55.68%、50.39%和28.56%;且在添加0.16 g/L的松油烯时,有两种三萜(去氢硫色多孔菌酸和去氢齿孔菌酸)在樟芝菌丝体中的含量显著升高,分别较对照组提高了5.56倍和0.96倍,在添加0.08 g/L的α-松油醇时,去氢硫色多孔菌酸的含量显著升高,较对照组提高了3.20倍。该研究发现了3种可以促进樟芝皿式培养三萜生成的单萜,为樟芝皿式培养提供了新的思路。
Antrodia cinnamomea is a kind of edible medicinal fungus.Triterpenoids are one of the dominant active ingredients in the fruiting bodies of A. cinnamomea, which have pharmacological effects such as anti-cancer, hepatoprotective and anti-inflammatory.Wild fruiting bodies of A. cinnamomea are only found on the inner rotten wall of Cinnamomum kanehirae, which leads to the fact that the active products of A. cinnamomea cannot satisfy the huge market demand.Petri-dish culture is a method for artificial cultivation of A. cinnamomea, which has the advantages of short cultivation time and simple operation.But the triterpenoids production of the A. cinnamomea mycelia in petri-dish culture is low.C. kanehirae is the only wild host of A. cinnamomea.Previous studies had indicated that extracts of C. kanehirae could promote the growth and triterpenoids production of mycelia in petri-dish culture.However, there are few reports on the effects of a single substance from C. kanehirae on triterpenoids synthesis of A. cinnamomea in petri-dish culture.To enhance the tirterpenoids production of A. cinnamomea mycelia, three monoterpenoids (α-terpineol, terpinene-4-ol and α-terpinene) from C. kanehirae were added in the medium in petri-dish culture.Biomass and triterpenoids yield of the mycelia were used as detection indices.The results indicated that α-terpineol was the only one among the three monoterpenoids had the ability to promote the growth of A. cinnamomea mycelia in petri-dish culture.The optimal adding concentration of α-terpineol was 0.08 g/L and the biomass of A. cinnamomea mycelia reached to 165.67 mg/plate.Three monoterpenoids all have the promotion effect on synthesis of triterpenoids in A. cinnamomea in petri-dish culture.The optimal adding concentration of α-terpineol is 0.08 g/L and the triterpenoids yield of A. cinnamomea mycelia reached to 17.55 mg/g with 50.39% increase compared with the control group, in the meanwhile, one triterpenoid, dehydrosulphurenic acid, was detected 3.20-fold increase compared with the control group.The optimal adding concentration of terpinene-4-ol is 0.12 g/L and the triterpenoids yield of A. cinnamomea mycelia reached to 8.89 mg/g with 28.65% increase compared with the control group.The optimal adding concentration of α-terpinene is 0.16 g/L and the triterpenoids yield of A. cinnamomea mycelia reached to 13.56 mg/g with 55.68% increase compared with the control group, in the meanwhile, two triterpenoids, dehydrosulphurenic acid and dehydroeburicoic acid, were detected 5.56-fold and 0.96-fold increase respectively compared with the control group.In this study, three monoterpenoids that could promote triterpenoids production of A. cinnamomea in petri-dish culture were identified and it provides new ideas for promoting triterpenoids synthesis of A. cinnamomea in petri-dish culture.
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