食品与发酵工业 >

2024 , Vol. 50 >Issue 9: 210 - 218

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.038350

研究报告

不同方法提取红鳍笛鲷鱼鳞胶原蛋白的结构和理化特性研究

  • 黄家宝 ,
  • 邓旗 ,
  • 薛梦凡 ,
  • 任雪婷 ,
  • 吴雨盈 ,
  • 孙力军 ,
  • 房志家 ,
  • 温婉宁
展开
  • 1(广东海洋大学 食品科技学院,广东省水产品加工与安全重点实验室,广东省海洋食品工程技术研究中心,广东省现代农业科技创新中心,广东省水产预制食品加工与品质控制工程技术研究中心,广东 湛江,524088)
    2(海洋食品精深加工关键技术省部共建协同创新中心,大连工业大学,辽宁 大连,116034)
第一作者: 硕士研究生(邓旗讲师为通信作者,E-mail:dengqi1024@163.com)

收稿日期: 2023-12-23

  修回日期: 2024-01-17

  网络出版日期: 2024-06-11

基金资助

国家自然科学基金青年科学基金项目(32302186)

收起

Characterization of collagens from scale of Lutjanus erythropterus by different extraction methods

  • HUANG Jiabao ,
  • DENG Qi ,
  • XUE Mengfan ,
  • REN Xueting ,
  • WU Yuying ,
  • SUN Lijun ,
  • FANG Zhijia ,
  • WEN Wanning
Expand
  • 1(College of Food Science and Technology, Guangdong Ocean University, Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Guangdong Provincial Engineering Technology Research Center of Seafood, Guangdong Provincial Engineering Technology Research Center of Aquatic Prepared Food Processing and Quality Control, Zhanjiang 524088, China)
    2(Collaborative Innovation Center of Seafood Deep Processing, Dalian Polytechnic University, Dalian 116034, China)

Received date: 2023-12-23

  Revised date: 2024-01-17

  Online published: 2024-06-11

Fold

摘要

为探明不同提取方法对红鳍笛鲷(Lutjanus erythropterus)鱼鳞胶原蛋白理化特性的影响,以红鳍笛鲷为原料,分别提取酸溶性胶原蛋白(acid soluble collagen,ASC)、酶溶性胶原蛋白(pepsin soluble collagen,PSC)及热水溶性胶原蛋白(hot water soluble collagen,HSC),对比分析3种胶原蛋白提取率、得率及纯度的差异,并测定其氨基酸组成、分子质量、二级结构、Zeta电位、溶解性以及微观结构等理化特性。结果表明,热水法提取率最高,为45.25%,其次为胃蛋白酶法;HSC、ASC、PSC的亚氨酸含量分别为18.28%、17.54%和18.30%。HSC、ASC和PSC均为I型胶原蛋白,包含两条α链(α1和α2),ASC比PSC的β链含量高,HSC、PSC的γ带消失;ASC、PSC具有相似的红外光谱,且保留了完整的三螺旋结构,HSC的三螺旋结构被破坏;HSC、ASC、PSC的净电荷零点为5~6;ASC、PSC在pH 5~11中溶解度较低;扫描电镜显示,ASC呈无序纤维状结构,PSC呈致密多孔网状结构,HSC则基本为薄片状。3种提取方法提取的胶原蛋白均有I型胶原蛋白特征,其结构特征及理化性质均存在差异,可根据需求选择特定的胶原蛋白提取方法用以开发特定的产品。

关键词： 红鳍笛鲷; 鱼鳞; 胶原蛋白; 理化性质; 结构表征

本文引用格式

黄家宝 , 邓旗 , 薛梦凡 , 任雪婷 , 吴雨盈 , 孙力军 , 房志家 , 温婉宁 . 不同方法提取红鳍笛鲷鱼鳞胶原蛋白的结构和理化特性研究[J]. 食品与发酵工业, 2024 , 50(9) : 210 -218 . DOI: 10.13995/j.cnki.11-1802/ts.038350

Abstract

To explore the impact of various extraction techniques on the physicochemical properties of collagen in Lutjanus erythropterus scales, acid-soluble collagen (ASC), pepsin-soluble collagen (PSC), and hot water-soluble collagen (HSC) were extracted.The extraction rates, yields, and purities of the three methods were compared and analyzed.The amino acid composition, molecular weight, secondary structure, Zeta potential, solubility, and microstructure of both collagens were evaluated.The findings revealed that the highest collagen extraction rate was achieved using the hot water method (45.25%), followed by the pepsin method.The imide acid content of HSC, ASC, and PSC was 18.28%, 17.54%, and 18.30%, respectively.All collagens were identified as type I collagen, consisting of two α chains (α1 and α2).ASC exhibited a higher β chain content than PSC, while both HSC and PSC lacked the γ band.ASC and PSC displayed similar IR spectra and maintained the intact triple helix structure, whereas hot water extraction resulted in triple helix degradation.Zeta potential analysis revealed a net charge of zero for HSC, ASC, and PSC within the pH range of 5-6.ASC and PSC exhibited high solubility within a neutral to alkaline pH range (5-11).Scanning electron microscope results illustrated that ASC possessed a disordered fibrous structure, PSC had a dense porous network structure, and HSC appeared primarily laminar.Although all three extraction methods yielded type I collagen, their structural and physicochemical attributes differed.Therefore, specific collagen extraction methods can be selected to develop diverse products based on specific requirements.

Key words： Lutjanus erythropterus; scale; collagen; physicochemical properties; structural characterization

参考文献

[1] CUI K, FU Z L, CHENG D C, et al.Development of immune functionality in larval and juvenile crimson snapper Lutjanus erythropterus (Bloch 1790)[J].Aquaculture Reports, 2018, 10:1-7.
[2] 冯波, 陈文河, 颜云榕.基于体重的北部湾红鳍笛鲷单位补充量渔获量与亲体生物量的研究[J].上海海洋大学学报, 2009, 18(4):460-465.
FENG B, CHEN W H, YAN Y R.A research on weight-based yield per recruit and spawning-biomass per recruit of Lutjanus erythropterus in Beibu Gulf[J].Journal of Shanghai Ocean University, 2009, 18(4):460-465.
[3] ZALL R.Unconventional techniques to deal with waste recovery or treatment schemes[J].Managing Food Industry Waste, 2004:105-113.
[4] ZHANG F X, WANG A N, LI Z H, et al.Preparation and characterisation of collagen from freshwater fish scales[J].Food and Nutrition Sciences, 2011, 2(8):818-823.
[5] SHOULDERS M D, RAINES R T.Collagen structure and stability[J].Annual Review of Biochemistry, 2009, 78:929-958.
[6] SIONKOWSKA A, SKRZYŃSKI S, ŚMIECHOWSKI K, et al.The review of versatile application of collagen[J].Polymers for Advanced Technologies, 2017, 28(1):4-9.
[7] 王娟娟, 刘勋, 周学, 等.胶原蛋白的研究进展及其应用[J].中国皮革, 2022, 51(7):1-7.
WANG J J, LIU X, ZHOU X, et al.Research progress and application of collagen[J].China Leather, 2022, 51(7):1-7.
[8] MATMAROH K, BENJAKUL S, PRODPRAN T, et al.Characteristics of acid soluble collagen and pepsin soluble collagen from scale of spotted golden goatfish (Parupeneus heptacanthus)[J].Food Chemistry, 2011, 129(3):1179-1186.
[9] 刘焱, 刘伦伦, 罗灿, 等.不同方法提取的草鱼鱼皮胶原蛋白性质比较[J].食品研究与开发, 2015, 36(8):34-39.
LIU Y, LIU L L, LUO C, et al.Property comparison of grass carp skin collagen extracted by different method[J].Food Research and Development, 2015, 36(8):34-39.
[10] 林海生, 王雯, 秦小明, 等.不同方法提取马氏珠母贝外套膜胶原蛋白理化性质比较[J].广东海洋大学学报, 2022, 42(1):106-112.
LIN H S, WANG W, QIN X M, et al.Characterization of collagens from mantle of Pinctada martensii by different extraction methods[J].Journal of Guangdong Ocean University, 2022, 42(1):106-112.
[11] 魏冉. 天然高分子基多孔止血海绵的制备及性能研究进展[J].化学推进剂与高分子材料, 2019, 17(6):20-27.
WEI R.Research progress in preparation and performance of natural polymer-based porous hemostatic sponge[J].Chemical Propellants & Polymeric Materials, 2019, 17(6):20-27.
[12] 田静. 胶原蛋白基人工皮肤的制备与性质研究[D].兰州:兰州大学, 2022.
TIAN J.Development and Characterization of Collagen-Based Artificial Skin[D].Lanzhou:Lanzhou University, 2022.
[13] 何会霞, 王莉莉, 蔡向东, 等.牦牛胶原蛋白-羟基磷灰石-海藻酸钠3D支架高效修复骨缺损[J].中国科学:化学, 2023, 53(9):1750-1760.
HE H X, WANG L L, CAI X D, et al.A 3D scaffold of yak collagen-hydroxyapatite-sodium alginate for efficiently repairing bone defects[J].Scientia Sinica Chimica, 2023, 53(9):1750-1760.
[14] 肖枫. 黄河鲤鱼鳞胶原蛋白的性质及胶原肽活性研究[D].镇江 江苏大学, 2014.
XIAO F.Study on the characterization of collagen and bioactive peptides from Cyprinus carpio haematopterus scale[D].Zhenjiang:Jiangsu University,2014.
[15] 曾芳. 鱼鳞胶原蛋白、明胶和羟基磷灰石综合提取研究[D].南昌:南昌大学, 2013.
ZENG F.Study on comprehensive extraction of fish scale collagen, gelatin and hydroxyapatite[D].Nanchang:Nanchang University, 2013.
[16] LIU W T, ZHANG Y, LI G Y, et al.Structure and composition of teleost scales from snakehead Channa argus (Cantor) (Perciformes:Channidae)[J].Journal of Fish Biology, 2008, 72(4):1055-1067.
[17] KNOTT L, BAILEY A J.Collagen cross-links in mineralizing tissues:A review of their chemistry, function, and clinical relevance[J].Bone, 1998, 22(3):181-187.
[18] NALINANON S, BENJAKUL S, KISHIMURA H.Collagens from the skin of arabesque greenling (Pleurogrammus azonus) solubilized with the aid of acetic acid and pepsin from albacore tuna (Thunnus alalunga) stomach[J].Journal of the Science of Food and Agriculture, 2010, 90(9):1492-1500.
[19] MINH THUY L, OKAZAKI E, OSAKO K.Isolation and characterization of acid-soluble collagen from the scales of marine fishes from Japan and Vietnam[J].Food Chemistry, 2014, 149:264-270.
[20] WANG L, AN X, XIN Z, et al.Isolation and characterization of collagen from the skin of deep-sea redfish (Sebastes mentella)[J].Journal of Food Science, 2007, 72(8):E450-E455.
[21] KAEWDANG O, BENJAKUL S, KAEWMANEE T, et al.Characteristics of collagens from the swim bladders of yellowfin tuna (Thunnus albacares)[J].Food Chemistry, 2014, 155:264-270.
[22] CHEN J D, LI L, YI R Z, et al.Extraction and characterization of acid-soluble collagen from scales and skin of tilapia (Oreochromis niloticus)[J].LWT-Food Science and Technology, 2016, 66:453-459.
[23] 陈涛, 李伟峰.红鳍笛鲷肌肉营养成分分析[J].海洋湖沼通报, 2016(6):67-72.
CHEN T, LI W F.Analysis of muscle nutrients of Lutjanus erythopterus[J].Transactions of Oceanology and Limnology, 2016(6):67-72.
[24] ZOU T B, HE T P, LI H B, et al.The structure-activity relationship of the antioxidant peptides from natural proteins[J].Molecules, 2016, 21(1):72.
[25] HE L, LAN W T, WANG Y, et al.Extraction and characterization of self-assembled collagen isolated from grass carp and crucian carp[J].Foods, 2019, 8(9):396.
[26] 黄宇玫, 李敏, 曾芳, 等.酸提和酶解两步法连续提取鳙鱼鱼鳞胶原蛋白工艺研究[J].食品科技, 2019, 44(2):152-158.
HUANG Y M, LI M, ZENG F, et al.Extraction process of bighead fish scale collagen by two-step method of acid extraction and enzymatic hydrolysis[J].Food Science and Technology, 2019, 44(2):152-158.
[27] WEI P, ZHENG H, SHI Z Y, et al.Isolation and characterization of acid-soluble collagen and pepsin-soluble collagen from the skin of hybrid sturgeon[J].Journal of Wuhan University of Technology-Mater.Sci.Ed., 2019, 34(4):950-959.
[28] 汲聪玲, 陆剑锋, 吕顺, 等.不同提取温度对白鲢鱼皮明胶理化性质的影响[J].食品科学, 2016, 37(9):117-122.
JI C L, LU J F, LYU S, et al.Effect of extraction temperature on physical and chemical properties of gelatin from silver carp skin[J].Food Science, 2016, 37(9):117-122.
[29] LIU Y W, MA D H, WANG Y H, et al.A comparative study of the properties and self-aggregation behavior of collagens from the scales and skin of grass carp (Ctenopharyngodon idella)[J].International Journal of Biological Macromolecules, 2018, 106:516-522.
[30] WU J L, GUO X B, LIU H, et al.Isolation and comparative study on the characterization of guanidine hydrochloride soluble collagen and pepsin soluble collagen from the body of surf clam shell (Coelomactra antiquata)[J].Foods, 2019, 8(1):11.
[31] SINTHUSAMRAN S, BENJAKUL S, KISHIMURA H.Comparative study on molecular characteristics of acid soluble collagens from skin and swim bladder of seabass (Lates calcarifer)[J].Food Chemistry, 2013, 138(4):2435-2441.
[32] TAN Y Q, CHANG S K C.Isolation and characterization of collagen extracted from channel catfish (Ictalurus punctatus) skin[J].Food Chemistry, 2018, 242:147-155.
[33] 何坤, 崔含蕊, 毛战强, 等.胶原蛋白海绵与常见纤维素海绵止血材料的理化性质及凝血效果对比研究[J].中国医学装备, 2023, 20(10):201-205.
HE K, CUI H R, MAO Z Q, et al.Comparative study of physicochemical properties and coagulation effects of collagen sponge and common cellulose sponge of hemostatic materials[J].China Medical Equipment, 2023, 20(10):201-205.
[34] ARUMUGAM G K S, SHARMA D, BALAKRISHNAN R M, et al.Extraction, optimization and characterization of collagen from sole fish skin[J].Sustainable Chemistry and Pharmacy, 2018, 9:19-26.
Options
文章导航

/

〈 〉

技术支持：北京玛格泰克科技发展有限公司