Saccharomyces cerevisiae always faces various environmental stresses during industrial-scale fermentation, including oxidation, high temperature, acid, ethanol, and hyperosmosis.Therefore, screening and breeding of strains with high tolerance to these stresses is of great significance for industrial applications.It is well known that DNA recombination enzyme, nucleic acid repair enzyme, and other proteins involved in nucleic acid repair pathway play important roles in tolerance to abiotic stresses.Based on that, the effects of endogenous nucleic acid excision and repair genes (RAD16, RAD7, RAD23 and RAD4) and exogenous genes (UVRA) on the tolerance of S.cerevisiae to different stresses were investigated by gene editing.The results showed that overexpression of RAD16, RAD7, RAD23 and RAD4 could improve the tolerance against osmotic stress in S.cerevisiae.Furthermore, overexpression of UVRA gene from bacterium Acetobacter pasteurii could also improve the tolerance against osmotic stress, suggesting that the function of UVRA was conserved between eukaryotic and procaryotic cells.This results were beneficial to improvement of the tolerance of S.cerevisiae strains, especially under high osmotic stress, and also provide a novel strategy to reveal the mechanism of tolerance in yeast.
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