食品与发酵工业 >

2024 , Vol. 50 >Issue 14: 220 - 226

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.036536

研究报告

木犀草素稳定的不同油相Pickering乳液稳态化差异性研究

  • 刘文文 ,
  • 申宇航 ,
  • 刘祉妤 ,
  • 姜悦伟 ,
  • 唐越
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  • 1(大连工业大学 食品学院,辽宁 大连,116034)
    2(国家海洋食品工程技术研究中心,辽宁 大连,116034)
第一作者：硕士研究生(唐越副教授为通信作者,E-mail:249913440@qq.com)

收稿日期: 2023-06-21

  修回日期: 2023-07-13

  网络出版日期: 2024-08-02

基金资助

辽宁省教育厅高等学校基本科研项目(LJKZ0547)

收起

Stability of luteolin-based Pickering emulsion constructed by different oil phases

  • LIU Wenwen ,
  • SHEN Yuhang ,
  • LIU Zhiyu ,
  • JIANG Yuewei ,
  • TANG Yue
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  • 1(School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China)
    2(National Engineering Research Center of Seafood, Dalian 116034, China)

Received date: 2023-06-21

  Revised date: 2023-07-13

  Online published: 2024-08-02

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摘要

该实验以木犀草素(luteolin,LUT)为乳化剂、分别以碳链长度和不饱和双键数量不同的的玉米油(corn oil,CO)、亚麻籽油(linseed oil,LO)、南极磷虾油(Antarctic krill oil,AKO)为油相,构筑包埋异硫氰酸苄酯(benzyl isothiocyanate,BITC)的Pickering乳液,通过对乳液流变特性、光稳定性、贮藏稳定性、微观结构、氧化稳定性和BITC保留率的考察,明确碳链长度和不饱和双键含量不同的油相对LUT稳定的Pickering乳液特性的影响。研究结果表明,LUT 稳定的Pickering乳液不会因油相中碳链长度和不饱和双键含量不同而影响其光稳定性;与LUT-CO和LUT-LO Pickering乳液相比,LUT-AKO Pickering乳液的表观黏度最大、空间结构更为均匀,贮藏14 d后平均粒径最小[(213.47±2.30) nm]、电位绝对值最大[(42.73±1.96) mV]、BITC包埋率最高[(65.25±1.45)%],但因AKO反应活性较强导致其Pickering乳液氧化稳定性略低;通过对LUT稳定的不同油相Pickering乳液稳态化差异性研究,明确碳链较长的AKO更适合作为制备稳定的Pickering乳液的油相,该研究为拓宽LUT稳定不同油相Pickering乳液的应用提供理论指导。

关键词： 木犀草素; 油相; Pickering乳液; 异硫氰酸苄酯; 稳定性

本文引用格式

刘文文 , 申宇航 , 刘祉妤 , 姜悦伟 , 唐越 . 木犀草素稳定的不同油相Pickering乳液稳态化差异性研究[J]. 食品与发酵工业, 2024 , 50(14) : 220 -226 . DOI: 10.13995/j.cnki.11-1802/ts.036536

Abstract

In this study, luteolin (LUT) was used as an emulsifier, benzyl isothiocyanate (BITC) as a model of nutrient delivery, and different oils [corn oil (CO), linseed oil (LO), and Antarctic krill oil (AKO)] with different carbon chain lengths and unsaturated double bond numbers as oil phases to construct Pickering emulsions.The rheological properties, photostability, storage stability, microstructure, oxidation stability, and BITC retention of Pickering emulsions were investigated to clarify the effects of different oil phases with carbon chain length and unsaturated double bond content on the properties of LUT-based Pickering emulsions.Results showed that the photostability of LUT-based Pickering emulsion was not affected by the different carbon chain lengths and unsaturated double bond content in the oil phases.Compared with LUT-CO and LUT-LO Pickering emulsion, LUT-AKO Pickering emulsion had the highest apparent viscosity and most uniform spatial structure.After 14 days of storage, the average particle size of LUT-AKO Pickering emulsion was the smallest [(213.47±2.30) nm], and the absolute value of Zeta potential and the embedding rate of BITC were the highest, reaching (42.73±1.96) mV and (65.25±1.45)%, respectively.The oxidation stability of LUT-AKO Pickering emulsion was slightly lower than those of other Pickering emulsions because of the strong AKO reactive activation.This study identified that AKO with a longer carbon chain was more suitable for preparing stable Pickering emulsions.This research provides a theoretical reference for expanding the application of LUT-based Pickering emulsions with different oil phases.

Key words： luteolin; oil phase; Pickering emulsion; benzyl isothiocyanate; stability

参考文献

[1] 王笑涵, 姜卉, 吴海涛, 等.大黄鱼卵分离蛋白乳液的构筑及其体外消化规律[J].食品科学, 2020, 41(18):8-13.
WANG X H, JIANG H, WU H T, et al.Construction and in vitro digestion properties of Pseudosciaena crocea roe protein isolate-stabilized emulsion[J].Food Science, 2020, 41(18):8-13.
[2] KHAMIS I, HEIKKILA J J.Effect of isothiocyanates, BITC and PEITC, on stress protein accumulation, protein aggregation and aggresome-like structure formation in Xenopus A6 kidney epithelial cells[J].Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 2018, 204:1-13.
[3] ROUSSEAU D.Fat crystals and emulsion stability—A review[J].Food Research International, 2000, 33(1):3-14.
[4] LIU Z Y, LIN S Y, LIU W W, et al.Construction of benzyl isothiocyanate-loaded fish skin gelatin-luteolin compound emulsion delivery system, and its digestion and absorption characteristics[J].Journal of the Science of Food and Agriculture, 2023, 103(4):1864-1873.
[5] TIAN T, TONG X H, YUAN Y, et al.Preparation of benzyl isothiocyanate nanoemulsions by different emulsifiers:Stability and bioavailability[J].Process Biochemistry, 2021, 111:128-138.
[6] LI F F, LI X H, HUANG K L, et al.Preparation and characterization of Pickering emulsion stabilized by hordein-chitosan complex particles[J].Journal of Food Engineering, 2021, 292:110275.
[7] 冯鑫, 马良, 戴宏杰, 等.食品级Pickering乳液的稳定性及β-胡萝卜素的装载研究[J].食品与发酵工业, 2021, 47(6):18-25.
FENG X, MA L, DAI H J, et al.The study on stability of food grade Pickering emulsion and the loading of β-carotene[J].Food and Fermentation Industries, 2021, 47(6):18-25.
[8] SHARKAWY A, BARREIRO M F, RODRIGUES A E.Chitosan-based Pickering emulsions and their applications:A review[J].Carbohydrate Polymers, 2020, 250:116885.
[9] SOUZA E M C, FERREIRA M R A, SOARES L A L.Pickering emulsions stabilized by zein particles and their complexes and possibilities of use in the food industry:A review[J].Food Hydrocolloids, 2022, 131(17):107781.
[10] 康芷若. 木犀草素纳米复合物的制备及药理学评价[D].沈阳:沈阳农业大学, 2022.
KANG Z R.Preparation and pharmacological evaluation of luteolin nanocomplexes[D].Shenyang:Shenyang Agricultural University, 2022.
[11] 葛思彤, 李琦, 贾睿, 等.基于玉米醇溶蛋白/没食子酸复合纳米颗粒提升玉米油Pickering乳液的氧化稳定性[J].食品科学, 2022, 43(20):78-85.
GE S T, LI Q, JIA R, et al.Zein/gallic acid composite nanoparticles to improve the oxidation stability of corn oil Pickering emulsion[J].Food Science, 2022, 43(20):78-85.
[12] SUN Y W, CHAI X H, HAN W J, et al.Pickering emulsions stabilized by hemp protein nanoparticles:Tuning the emulsion characteristics by adjusting anti-solvent precipitation[J].Food Hydrocolloids, 2023, 138:108434.
[13] MA P H, ZENG Q H, TAI K D, et al.Preparation of curcumin-loaded emulsion using high pressure homogenization:Impact of oil phase and concentration on physicochemical stability[J].LWT-Food Science and Technology, 2017, 84:34-46.
[14] SHI J Y, XIAO J X, LIU L, et al.Ultrasonic assisted oil-in-water emulsions stabilized by flaxseed protein isolate:Influence of different oils[J].Journal of Dispersion Science and Technology, 2022, 43(12):1789-1800.
[15] 霍超, 纳文娟, 徐桂花.玉米油的主要功效成分及开发应用前景[J].中国食物与营养, 2008, 14(10):37-38.
HUO C, NA W J, XU G H. Health function of corn oil and its utilization[J].Food and Nutrition in China, 2008, 14(10):37-38.
[16] 廖振林,李倩滢, 陈俊杰, 等.亚麻籽油组分的功能活性研究进展[J].现代食品科技, 2021, 37(11):379-389;337.
LIAO Z L, LI Q Y, CHEN J J, et al.Research progress on the functional activity of flaxseed oil components[J].Modern Food Science and Technology, 2021, 37(11):379-389;337.
[17] 田晓清, 杨桥, 邵盛男, 等.南极磷虾脂溶性成分的研究进展[J].海洋渔业, 2011, 33(4):462-466.
TIAN X Q, YANG Q, SHAO S N, et al.The research progress of ester-soluble constituents of Antarctic Krill [J].Marine Fisheries, 2011, 33(4):462-466.
[18] WANG L, LU S M, DENG Y P, et al.Pickering emulsions stabilized by luteolin micro-nano particles to improve the oxidative stability of pine nut oil[J].Journal of the Science of Food and Agriculture, 2021, 101(4):1314-1322.
[19] PI G L, LI Y M, BAO M T, et al.Novel and environmentally friendly oil spill dispersant based on the synergy of biopolymer xanthan gum and silica nanoparticles[J].ACS Sustainable Chemistry and Engineering, 2016, 4(6):3095-3102.
[20] ZHANG X Y, LIU Z Y, WANG A L, et al.Influence of fish skin gelatin-sodium alginate complex stabilized emulsion on benzyl isothiocyanate stability and digestibility in vitro and in vivo[J].Journal of the Science of Food and Agriculture, 2022, 102(13):5680-5689.
[21] TANG Y, WANG X H, YU J H, et al.Fish skin gelatin-based emulsion as a delivery system to protect lipophilic bioactive compounds during in vitro and in vivo digestion:The case of benzyl isothiocyanate[J].LWT-Food Science and Technology, 2020, 134(2):110145.
[22] 吴梓宣. 黄渤海主要扇贝暂养净化及贮藏过程中脂质变化及控制[D].大连:大连工业大学, 2019.
WU Z X.Study on changes of lipids and their control in major scallops from the yellow sea and the bohai sea during aquaculture purification and storage[D].Dalian:Dalian Polytechnic University, 2019.
[23] 付冬文. Pickering型和传统剂型DHA藻油乳液体系的构建及产品开发[D].南昌:南昌大学, 2020.
FU D W.Construction of Pickering and traditional DHA algal oil emulsion systems and its product development[D].Nanchang:Nanchang University, 2020.
[24] WANG W N, WANG R Y, YAO J, et al.Effect of ultrasonic power on the emulsion stability of rice bran protein-chlorogenic acid emulsion[J].Ultrasonics Sonochemistry, 2022, 84:105959.
[25] TAHERIAN A R, FUSTIER P, BRITTEN M, et al.Rheology and stability of beverage emulsions in the presence and absence of weighting agents:A review[J].Food Biophysics, 2008, 3(3):279-286.
[26] 王璐. 花生果实荚壳中木犀草素的提取纯化、纳米粒制备及其稳定Pickering乳液的设计与研究[D].哈尔滨:东北林业大学, 2020.
WANG L.Extraction and purification of luteolin from peanut shell, preparation of nanoparticles and design and study of stable Pickering emulsion[D].Harbin:Northeast Forestry University, 2020.
[27] ZOU H N, ZHAO N, SUN S, et al.High-intensity ultrasonication treatment improved physicochemical and functional properties of mussel sarcoplasmic proteins and enhanced the stability of oil-in-water emulsion[J].Colloids and Surfaces A Physicochemical and Engineering Aspects, 2020, 589:124463.
[28] PERIASAMY V S, ATHINARAYANAN J, ALSHATWI A A.Anticancer activity of an ultrasonic nanoemulsion formulation of Nigella sativa L.essential oil on human breast cancer cells[J].Ultrasonics Sonochemistry, 2016, 31:449-455.
[29] TEO Y Y, MISRAN M, LOW K H, et al.Effect of unsaturation on the stability of C18 polyunsaturated fatty acids vesicles suspension in aqueous solution[J].Bulletin of the Korean Chemical Society, 2011, 32(1):59-64.
[30] 张翔宇, 王瑷琳, 刘祉妤, 等.鱼皮明胶-海藻酸钠相互作用及其规律[J].食品与发酵工业, 2022, 48(7):160-165.
ZHANG X Y, WANG A L, LIU Z Y, et al.Interaction and rule between sodium alginate and fish skin gelatin[J].Food and Fermentation Industries, 2022, 48(7):160-165.
[31] 徐明进, 李明远, 彭勃, 等.Zeta电位和界面膜强度对水包油乳状液稳定性影响[J].应用化学, 2007, 24(6):623-627.
XU M J, LI M Y, PENG B, et al.Effects of strength of interfacial film and zeta potential on oil-inwater emulsion stability[J].Chinese Journal of Applied Chemistry, 2007, 24(6):623-627.
[32] 刘芸杭. 南极磷虾油分级提取及南极磷虾油含量对乳液制备的影响 [D].大连:大连工业大学, 2021.
LIU Y H.Fractional extraction of Antarctic krill oil and the effect of Antarctic krill oil content on emulsion preparation [D].Dalian:Dalian Polytechnic University, 2021.
[33] WU T, MAO L C.Influences of hot air drying and microwave drying on nutritional and odorous properties of grass carp (Ctenopharyngodon idellus) fillets[J].Food Chemistry, 2008, 110(3):647-653.
[34] 汤丽霞, 沈恂.生物系统中脂质过氧化检测方法的评述[J].生物化学与生物物理进展, 1995, 22(2):108-112.
TANG L X, SHEN X.A review of the method in measuring lipid peroxidation in biological systems[J].Progress in Biochemistry and Biophysics, 1995, 22(2):108-112.
[35] 袁玥, 许兆滨, 杨宪时, 等.温度对南极磷虾粉贮藏性的影响[J].食品工业科技, 2015, 36(3):327-330.
YUAN Y, XU Z B, YANG X S, et al.Effect of temperature on the storage properties of Antarctic krill meal[J].Science and Technology of Food Industry, 2015, 36(3):327-330.
[36] 朱波, 蒋将, 李进伟, 等.三种不同脂肪酸组成的油脂乳化液的理化性质研究[J].食品工业科技, 2013, 34(14):103-107;112.
ZHU B, JIANG J, LI J W, et al.Physicochemical properties of three different oil in water emulsions[J].Science and Technology of Food Industry, 2013, 34(14):103-107;112.
[37] KIM Y J, KIM B K, LEE M H, et al.Improving curcumin retention in oil-in-water emulsions coated by chitosan and their disperse stability exposed to thermal treatments[J].Journal of Food Engineering, 2022, 319:110918.
[38] 姜卉. 大黄鱼卵分离蛋白乳液输送系统的构筑及吸收利用率的研究 [D].大连:大连工业大学, 2019.
JIANG H.Construction and bioaccessibility of Pseudosciaena crocea roe protein isolate emulsion delivery system [D].Dalian:Dalian Polytechnic University, 2019.
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