食品与发酵工业 >

2024 , Vol. 50 >Issue 14: 314 - 320

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.037583

分析与检测

芬太尼类物质的荧光适配体传感器构建及应用

  • 姜玉北 ,
  • 熊玥 ,
  • 郭婷 ,
  • 周莹 ,
  • 刘晓竹 ,
  • 杨黎华 ,
  • 马良
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  • 1(西南大学 食品科学学院,重庆,400715)
    2(川渝共建特色食品重庆市重点实验室,重庆,400715)
    3(佛山微奥云生物技术有限公司,广东 佛山,528000)
    4(毒品分析及禁毒技术公安部重点实验室,云南 昆明,650223)
    5(智慧禁毒教育部重点实验室,云南 昆明,650223)
    6(云南省智慧禁毒重点实验室,云南 昆明,650223)
    7(国家市场监管重点实验室(调味品监管技术),重庆,401121)
第一作者：硕士研究生(马良教授为通信作者,E-mail:zhyhml@163.com)

收稿日期: 2023-10-08

  修回日期: 2023-11-08

  网络出版日期: 2024-08-02

基金资助

毒品分析及禁毒技术公安部重点实验室开放课题计划项目(ynpc2022kf004);佛山市科技创新项目(2018IT100152);重庆英才计划(cstc2021ycjh-bgzxm0237)

收起

Construction and application of fluorescent aptamer sensor for fentanyl-related substances

  • JIANG Yubei ,
  • XIONG Yue ,
  • GUO Ting ,
  • ZHOU Ying ,
  • LIU Xiaozhu ,
  • YANG Lihua ,
  • MA Liang
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  • 1(College of Food Science, Southwest University, Chongqing 400715, China)
    2(Chongqing Key Laboratory of Specialty Food Co-Built by Sichuan and Chongqing, Chongqing 400715, China)
    3(Foshan Micro Miracles Biotechnology Company, Foshan 528000, China)
    4(Key Lab of Narcotics Assay and Control Technology, Ministry of Public Security, Kunming 650223, China)
    5(Yunnan Key Laboratory of Smart Drugs Control, Kunming 650223, China)
    6(Key Laboratory of Smart Drugs Control (Yunnan Police College), Kunming 650223, China)
    7(Key Laboratory of Condiment Supervision Technology for State Market Regulation, Chongqing 401121, China)

Received date: 2023-10-08

  Revised date: 2023-11-08

  Online published: 2024-08-02

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摘要

近年来报道,新精神活性物质伪装成零食、饮料等形式进入并潜伏在大众日常生活中。其中芬太尼类物质是典型代表,市场监管和公安侦查均迫切需要现场快速筛查技术和产品的支撑。基于适配体的快速检测技术发展迅速,然而特异性芬太尼类物质适配体研究相对缺乏。该文以芬太尼类物质的核心结构类似物的裁剪适配体为识别元件,以Fe3O4作为猝灭剂和磁分离剂,利用荧光猝灭技术构建适配体传感平台,对芬太尼类物质实现广谱识别。结果表明,该荧光传感器检测芬太尼类物质为100~500 μg/L,R为0.997 7,检测限为19.7 μg/L,相对标准偏差为5.91%~7.51%,在非居民用水和居民用水中加标回收率分别为81.14%~91.54%和106.7%~117.4%,可用于水样芬太尼类物质的快速筛查,填补芬太尼总量广谱快速检测方面研究的空白。

关键词： 芬太尼; 快速筛查; 广谱检测; 适配体; 荧光传感器

本文引用格式

姜玉北 , 熊玥 , 郭婷 , 周莹 , 刘晓竹 , 杨黎华 , 马良 . 芬太尼类物质的荧光适配体传感器构建及应用[J]. 食品与发酵工业, 2024 , 50(14) : 314 -320 . DOI: 10.13995/j.cnki.11-1802/ts.037583

Abstract

In recent years, it has been reported that new psychoactive substances disguised as snacks, drinks, and other forms enter and lurk in the daily life of the public.Among them, fentanyl-related substances are typical representatives.Market supervision and public security investigation urgently need the support of on-site rapid screening technology and products.The rapid detection technology based on aptamers has developed rapidly, but the research on specific fentanyl aptamers is relatively lacking.In this study, tailoring aptamers of core structure analogues of fentanyl-related substances were used as recognition elements, Fe3O4 was used as quencher and magnetic separator, and fluorescence quenching technology was used to build an aptamer sensing platform to achieve broad spectrum recognition of the total amount of fentanyl.Results showed that the fluorescence sensor detected fentanyl in the range of 100-500 μg/L, R was 0.997 7, the detection limit was 19.7 μg/L, and the relative standard deviation was 5.91%-7.51%.The standard recoveries of non-residential water and residential water were 81.14%-91.54% and 106.7%-117.4%, respectively.This developed strategy can be used for the rapid screening of water samples of fentanyl and enrich the research on the wide-spectrum rapid detection of fentanyl-related substances.

Key words： fentanyl; rapid screening; broad spectrum detection; aptamer; fluorescence sensor

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