食品与发酵工业 >

2024 , Vol. 50 >Issue 14: 328 - 333

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.037020

分析与检测

基于金纳米粒子荧光探针可视化快速检测复杂样品中氰化物

  • 马文娟 ,
  • 李蕊岑 ,
  • 袁彩霞 ,
  • 洪霞 ,
  • 王玉 ,
  • 何海宁
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  • 1(甘肃中商食品质量检验检测有限公司,甘肃 兰州,730010)
    2(甘肃省商业科技研究所有限公司,甘肃 兰州,730010)
    3(甘肃省平凉市食品检验检测中心,甘肃 平凉,744000)
第一作者：学士,工程师(袁彩霞高级工程师为通信作者,E-mail:13919219511@163.com)

收稿日期: 2023-08-10

  修回日期: 2023-10-17

  网络出版日期: 2024-08-02

基金资助

甘肃省市场监督管理局科技计划项目(SSCJG-SP-A202202)

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Visualized and rapid detection of cyanide in complex samples using fluorescence probes based on gold nanoparticles

  • MA Wenjuan ,
  • LI Ruicen ,
  • YUAN Caixia ,
  • HONG Xia ,
  • WANG Yu ,
  • HE Haining
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  • 1(Gansu Zhongshang Food Quality Test and Detection Co.Ltd., Lanzhou 730010, China)
    2(Gansu Business Science and Technology Institute Co.Ltd., Lanzhou 730010, China)
    3(Gansu Pingliang Food Inspection and Testing Center, Pingliang 744000, China)

Received date: 2023-08-10

  Revised date: 2023-10-17

  Online published: 2024-08-02

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摘要

该研究利用水溶性的荧光素异硫氰酸酯(fluorescein isothiocyanate,FITC)覆盖石墨烯负载金纳米粒子复合材料,使探针分子FITC的荧光猝灭。然后,通过向体系中加入氰化物,氰根离子(CN-)对金纳米粒子的强络合作用形成稳定的络合物Au(CN)3-x而使探针分子荧光逐渐恢复,以此实现氰化物的快速检测。对试验条件溶液pH值、反应时间、干扰离子进行了优化。试验结果表明,在H2O2存在下,基于FITC-Au NPs荧光探针对CN-最低检测限达到了0.032 μmol/L,在0.05~80 μmol/L内具有良好的线性关系。利用FITC-Au NPs传感探针的高稳定性和选择性实现了复杂样品中CN-的快速检测。这种简单、快速、经济的荧光传感系统在实际样品中阴离子的检测方面显示出巨大的实用潜力。

关键词： 荧光探针; 氰化物; 杏仁露; 异硫氰酸酯; 金纳米粒子

本文引用格式

马文娟 , 李蕊岑 , 袁彩霞 , 洪霞 , 王玉 , 何海宁 . 基于金纳米粒子荧光探针可视化快速检测复杂样品中氰化物[J]. 食品与发酵工业, 2024 , 50(14) : 328 -333 . DOI: 10.13995/j.cnki.11-1802/ts.037020

Abstract

This study utilized water-soluble fluorescein isothiocyanate (FITC) to cover graphene loaded gold nanoparticle composites, and the fluorescence of the probe molecule FITC was quenched.Then, by adding cyanide to the system, the strong complexation of cyanide ions (CN-) with gold nanoparticles formed a stable complex Au (CN)3-x, gradually restoring the fluorescence of the probe molecules, thereby achieving rapid detection of cyanide.The pH value of the experimental solution, reaction time, and interfering ions were optimized.The experimental results indicated that the FITC-Au NPs fluorescence probe had a minimum detection limit of 0.036 μmol/L for CN- in the presence of an H2O2 environment.There was a good linear relationship within the 0.05-80 μmol/L concentration range.The high stability and selectivity of FITC-Au NPs probes were utilized to achieve rapid detection of CN- in complex samples.This simple, fast, and economical fluorescence sensing system shows great practical potential in the detection of anions in actual samples.

Key words： fluorescence probe; cyanide; almond dew; isothiocyanate ester; Au nanoparticle

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