食品与发酵工业 >

2024 , Vol. 50 >Issue 15: 41 - 47

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.037031

研究报告

黑枸杞本草酵素缓解小鼠脂溢性脱发作用

  • 闵祥博 ,
  • 李丽娜 ,
  • 余萍 ,
  • 李晶 ,
  • 惠美星 ,
  • 白淼
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  • 1(江西仁仁健康微生态科技有限公司,江西 樟树,331200)
    2(渤海大学 食品科学与工程学院,辽宁 锦州,121013)
第一作者：硕士,工程师(通信作者,E-mail:303817118@qq.com)

收稿日期: 2023-08-13

  修回日期: 2023-09-06

  网络出版日期: 2024-08-21

基金资助

北京伍连德公益基金会项目(WLD-2022062802)

收起

Alleviation of black wolfberry ferment on seborrheic alopecia in mice

  • MIN Xiangbo ,
  • LI Lina ,
  • YU Ping ,
  • LI Jing ,
  • HUI Meixing ,
  • BAI Miao
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  • 1(Jiangxi Renren Health Microecological Technology Co.Ltd., Zhangshu 331200, China)
    2(School of Food Science and Engineering, Bohai University, Jinzhou 121013, China)

Received date: 2023-08-13

  Revised date: 2023-09-06

  Online published: 2024-08-21

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摘要

该研究对黑枸杞本草酵素缓解小鼠脂溢性脱发症状的功能开展了研究。将实验小鼠随机分为空白组、模型组、原浆组和酵素组,通过皮下注射丙酸睾酮建立脂溢性脱发小鼠模型,原浆组和酵素组小鼠在14 d内分别灌胃黑枸杞原浆和黑枸杞本草酵素(25 mL/kg·bw),观察其对脱发小鼠背部毛发生长状态、皮肤组织中毛囊数量、胶原蛋白含量、毛囊增殖细胞和毛囊干细胞标记物的影响。结果表明,黑枸杞原浆和酵素促进了脱发小鼠的毛发生长速度,增加了毛囊数量和胶原蛋白含量,显著提高了毛囊增殖细胞标记物Ki67和毛囊干细胞标记物Sox9的阳性表达 (P<0.05),且酵素组效果更明显。代谢组学结果表明黑枸杞原浆发酵后11种花色苷类代谢物上调。富含花色苷的黑枸杞本草酵素可能通过调控小鼠背部毛囊生长、胶原蛋白积累、毛囊增殖细胞和毛囊干细胞标记物的阳性表达,来缓解脂溢性脱发对小鼠毛发的生长,具备开发为功能性发酵食品的发展潜力。

关键词： 黑枸杞; 酵素; 脂溢性脱发; 毛囊; 代谢组学

本文引用格式

闵祥博 , 李丽娜 , 余萍 , 李晶 , 惠美星 , 白淼 . 黑枸杞本草酵素缓解小鼠脂溢性脱发作用[J]. 食品与发酵工业, 2024 , 50(15) : 41 -47 . DOI: 10.13995/j.cnki.11-1802/ts.037031

Abstract

This study investigated the functional effects of black wolfberry ferment in alleviating seborrheic alopecia symptoms in mice.Mice were randomly divided into control group, model group, puree group, and ferment group.A seborrheic alopecia mouse model was successfully established by subcutaneous injection of propionate testosterone.Mice in puree and ferment groups were orally administered black wolfberry fruit puree and black wolfberry fermentation liquid at 25 mL/kg·bw in 14 d, respectively.The effects on hair growth status, the number of hair follicles, collagen content, hair follicle proliferative cells, and hair follicle stem cell markers were assessed.The results indicated that both black wolfberry fruit puree and black wolfberry ferment promoted the hair growing rates, increased the number of hair follicles and the content of collagen, significantly enhanced the positive expression of the proliferative cell marker Ki67 and hair follicle stem cell marker Sox9 (P<0.05), with more pronounced effects observed in the ferment group.Metabolomics results revealed that eleven anthocyanin metabolites were upregulated in black wolfberry ferment group.The black wolfberry ferment, rich in anthocyanins, may alleviate seborrheic alopecia by regulating hair follicles growth, collagen accumulation, as well as the positive expression of hair follicle proliferative cells and hair follicle stem cells, thus possessing the potential to be developed into functional fermented foods.

Key words： black wolfberry; ferment; seborrheic alopecia; hair follicle; metabolomics

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