菊苣作为一种药食同源的食品,极具开发潜力。该研究利用菊苣叶提取物作为还原剂和稳定剂,采用一步法生物合成纳米银抑菌剂,为菊苣叶的开发利用提供了一条新的途径。通过单因素试验探讨了AgNO3与菊苣叶提取物体积比、反应pH、反应时间等对纳米银制备的影响。优化后的合成产物经紫外-可见吸收光谱、透射电镜、X射线衍射分析和傅立叶变换红外光谱等进行表征和鉴定,结果表明产物在426 nm附近有一明显的纳米银等离子体子共振特征吸收峰,纳米银颗粒呈球形,平均粒径为11.22 nm,具有良好的分散性,为面心立方晶型。抗氧化实验表明,菊苣叶生物合成纳米银与原菊苣叶提取物相比具有较高的DPPH自由基清除活性,AgNPs的IC50为0.053 mg/mL;对4-硝基苯酚的催化反应速率常数(0.119 8 min-1)、甲基橙(0.189 min-1)、罗丹明B(0.567 min-1)、亚甲基蓝(0.098 6 min-1),综上所述,利用菊苣叶提取物生物合成的纳米银具有一定抗氧化活性和催化活性,有望在临床、有机废水处理领域得到广泛应用。
Chicory, as a food with the same origin of food and medicine, has great development potential.Using the extract of chicory leaves as a reducing agent and a stabilizer, a silver nano-bacteriostatic agent was synthesized by one-step method, which provided a new way for the development and utilization of chicory leaves.The effects of AgNO3/Cichorium intybus extract volume ratio, pH and reaction time on the preparation of silver nanoparticles were investigated by single factor experiment.The optimized product was characterized and identified by UV-vis absorption spectrum, transmission electron microscopy, and X-ray diffraction analysis.Results showed that there was an obvious absorption peak at 426 nm, the silver nanoparticles were spherical, the average particle size was 11.22 nm, and had good dispersion, it was face-centered cubic crystal.The anti-oxidation experiment showed that the biosynthetic silver nanoparticles had higher DPPH free radical scavenging activity than the original extract of cichorium intybus leaves.The IC50 value of AgNPs was 0.053 mg/mL.The reaction rate constant of 4-nitrophenol could reach 0.119 8 min-1, methyl orange (0.189 min-1), rhodamine B (0.567 min-1), and methylene blue (0.098 6 min-1), nano-silver synthesized from chicory leaf extract had a certain antioxidant and catalytic activity and was expected to be widely used in clinical and organic wastewater treatment.
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