食品与发酵工业 >

2024 , Vol. 50 >Issue 16: 285 - 292

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.039458

分析与检测

蜂蜜中链霉素仿生印迹电化学传感检测方法

  • 李兆周 ,
  • 赵明辉 ,
  • 张冉 ,
  • 陈秀金 ,
  • 王耀 ,
  • 万宁波 ,
  • 牛华伟 ,
  • 高红丽 ,
  • 李芳 ,
  • 于慧春 ,
  • 袁云霞 ,
  • 吴影 ,
  • 李佩艳
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  • 1(河南科技大学 食品与生物工程学院,河南 洛阳,471000)
    2(河南省食品绿色加工与质量安全控制国际联合实验室,河南 洛阳,471000)
    3(食品加工与安全国家级实验教学示范中心,河南 洛阳,471000)
第一作者：博士,教授(陈秀金副教授为通信作者,E-mail:chenxiujin9610@126.com)

收稿日期: 2024-04-04

  修回日期: 2024-04-29

  网络出版日期: 2024-09-19

基金资助

洛阳市公益性行业科研专项(2202021A);河南省科技攻关项目(242102411001,232102320298);河南省优秀青年科学基金(202300410121);国家自然科学基金(31701694,U1504330,31702218);河南省青年人才托举工程项目(2020HYTP029);河南省研究生教育改革与质量提升工程项目(HNYJS2020JD06)

收起

Biomimetic imprinted electrochemical sensing detection of streptomycin in honey

  • LI Zhaozhou ,
  • ZHAO Minghui ,
  • ZHANG Ran ,
  • CHEN Xiujin ,
  • WANG Yao ,
  • WAN Ningbo ,
  • NIU Huawei ,
  • GAO Hongli ,
  • LI Fang ,
  • YU Huichun ,
  • YUAN Yunxia ,
  • WU Ying ,
  • LI Peiyan
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  • 1(College of Food and Bioengineering, Henan University of Science and Technology, Luoyang 471000, China)
    2(Henan International Joint Laboratory of Food Green Processing and Quality Safety Control, Luoyang 471000, China)
    3(National Demonstration Center for Experimental Food Processing and Safety Education, Luoyang 471000, China)

Received date: 2024-04-04

  Revised date: 2024-04-29

  Online published: 2024-09-19

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摘要

动物源食品中链霉素残留危害严重,建立高性能分析方法是确保食品安全和人类健康的必要手段。仿生印迹电化学传感检测方法具有高效、特异和灵敏等优点,已成为食品安全检测技术研究的重要领域。该研究以链霉素为模板分子,氧化石墨烯和壳聚糖为增敏材料,3-氨基苯基硼酸和邻苯二胺为复合功能单体,采用电聚合法构建了仿生印迹聚合物传感界面,建立了蜂蜜中链霉素的仿生印迹电化学传感检测方法。通过扫描电镜对印迹传感界面进行了表征,采用循环伏安法、差分脉冲伏安法和电化学交流阻抗法对仿生印迹传感器的电化学性能进行表征。在最佳反应条件下,该传感器的检测限为2.59×10-12 mol/L,线性范围为1.0×10-10~1.0×10-6 mol/L,洋槐蜜、枣花蜜和桂花蜜样品的加标回收率分别为94.78%~106.24%、93.65%~103.81%和97.19%~106.18%,相对标准偏差≤4.28%。试验结果表明,该方法具有良好的重复性、稳定性和重现性,能够满足蜂蜜中链霉素残留分析的需要。相关研究为复杂基质中痕量靶标的高特异性和超灵敏分析提供了技术支撑,对于蜂蜜中链霉素的快速筛查和安全监测具有重要意义。

关键词： 蜂蜜; 链霉素; 仿生印迹; 电化学传感器

本文引用格式

李兆周 , 赵明辉 , 张冉 , 陈秀金 , 王耀 , 万宁波 , 牛华伟 , 高红丽 , 李芳 , 于慧春 , 袁云霞 , 吴影 , 李佩艳 . 蜂蜜中链霉素仿生印迹电化学传感检测方法[J]. 食品与发酵工业, 2024 , 50(16) : 285 -292 . DOI: 10.13995/j.cnki.11-1802/ts.039458

Abstract

There has been a serious hazard for streptomycin residues in animal-derived foods.It is necessary to establish a high-performance analytical method to ensure food safety and human health.The biomimetic imprinting electrochemical sensing detection method has the advantages of high efficiency, specificity, and sensitivity, and has become an important area of research in the field of food safety detection technology.In this study, a biomimetic imprinted sensing interface was constructed by electropolymerization with streptomycin as the template, graphite oxide and chitosan as the sensitizing materials, and 3-aminobenzeneboronic acid and O-phenylenediamine as the composite functional monomers.The biomimetic imprinted electrochemical sensing detection method was then developed and applied for STR residue analysis in honey.The imprinted interface was characterized by scanning electron microscopy, and the electrochemical performance of the proposed sensor was evaluated with cyclic voltammetry, differential pulse voltammetry, and electrochemical impedance analysis.Under the optimal reaction conditions, the linear range was calculated as 1.0×10-10-1.0×10-6 mol/L with a detection limit of 2.59×10-12 mol/L, the recoveries of sophora nectar, jujube nectar, and osmanthus nectar were 94.78%-106.24%, 93.65%-103.81%, and 97.19%-106.18%, respectively, and the relative standard deviation was less than or equal to 4.28%.The experimental results show that this method has good repeatability, stability, and reproducibility.The related research provides technical support for the high specificity and ultrasensitive analysis of trace target compounds in complex matrices, which is of great significance for the rapid screening and safety monitoring of streptomycin in honey.

Key words： honey; streptomycin; biomimetic imprinting; electrochemical sensor

参考文献

[1] SCHATZ A, BUGLE E, WAKSMAN S A. Streptomycin, a substance exhibiting antibiotic activity against gram-positive and gram-negative bacteria[J]. Experimental Biology and Medicine, 1944, 55(1):66-69.
[2] 陈溪, 曲世超, 黄大亮, 等. 链霉素在动物体内残留和代谢的研究进展[J]. 检验检疫学刊, 2015, 25(4):49-51.
CHEN X, QU S C, HUANG D L, et al. Research progress on residues and metabolism of streptomycin in animals[J]. Journal of Inspection and Quarantine, 2015, 25(4):49-51.
[3] 江虹, 黄冬梅. 金莲橙OO与链霉素的显色反应及应用[J]. 化学试剂, 2008, 30(2):129-131.
JIANG H, HUANG D M. Color reactions of tropeolin OO with streptomycin and their analytical applications[J]. Chemical Reagents, 2008, 30(2):129-131.
[4] FRUGERI P M, DO LAGO A C, WISNIEWSKI C, et al. A spectrophotometric flow injection system for streptomycin determination in veterinary samples[J]. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 2014, 117:304-308.
[5] WANG X R, YANG S P, LI Y, et al. Optimization and application of parallel solid-phase extraction coupled with ultra-high performance liquid chromatography-tandem mass spectrometry for the determination of 11 aminoglycoside residues in honey and royal jelly[J]. Journal of Chromatography A, 2018, 1542:28-36.
[6] IANNI F, PUCCIARINI L, CAROTTI A, et al. Hydrophilic interaction liquid chromatography of aminoglycoside antibiotics with a diol-type stationary phase[J]. Analytica Chimica Acta, 2018, 1044:174-180.
[7] KOWALSKI P, OLĘDZKA I, LAMPARCZYK H. Capillary electrophoresis in analysis of veterinary drugs[J]. Journal of Pharmaceutical and Biomedical Analysis, 2003, 32(4-5):937-947.
[8] MORENO-GONZÁLEZ D, LARA F J, JURGOVSKÁ N, et al. Determination of aminoglycosides in honey by capillary electrophoresis tandem mass spectrometry and extraction with molecularly imprinted polymers[J]. Analytica Chimica Acta, 2015, 891:321-328.
[9] DÍEZ C, GUILLARME D, STAUB SPÖRRI A, et al. Aminoglycoside analysis in food of animal origin with a zwitterionic stationary phase and liquid chromatography-tandem mass spectrometry[J]. Analytica Chimica Acta, 2015, 882:127-139.
[10] GREMILOGIANNI A M, MEGOULAS N C, KOUPPARIS M A. Hydrophilic interaction vs ion pair liquid chromatography for the determination of streptomycin and dihydrostreptomycin residues in milk based on mass spectrometric detection[J]. Journal of Chromatography A, 2010, 1217(43):6646-6651.
[11] ARSAND J B, JANK L, MARTINS M T, et al. Determination of aminoglycoside residues in milk and muscle based on a simple and fast extraction procedure followed by liquid chromatography coupled to tandem mass spectrometry and time of flight mass spectrometry[J]. Talanta, 2016, 154:38-45.
[12] DU B Y, WEN F, GUO X D, et al. Evaluation of an ELISA-based visualization microarray chip technique for the detection of veterinary antibiotics in milk[J]. Food Control, 2019, 106:106713.
[13] DU B Y, WEN F, ZHANG Y D, et al. Presence of tetracyclines, quinolones, lincomycin and streptomycin in milk[J]. Food Control, 2019, 100:171-175.
[14] WEI D L, ZHANG X Y, CHEN B, et al. Using bimetallic Au@Pt nanozymes as a visual tag and as an enzyme mimic in enhanced sensitive lateral-flow immunoassays: Application for the detection of streptomycin[J]. Analytica Chimica Acta, 2020, 1126:106-113.
[15] ALHAMMADI M, ALIYA S, UMAPATHI R, et al. A simultaneous qualitative and quantitative lateral flow immunoassay for on-site and rapid detection of streptomycin in pig blood serum and urine[J]. Microchemical Journal, 2023, 195:109427.
[16] WU J X, ZHANG S E, ZHOU X P. Monoclonal antibody-based ELISA and colloidal gold-based immunochromatographic assay for streptomycin residue detection in milk and swine urine[J]. Journal of Zhejiang University. Science. B, 2010, 11(1):52-60.
[17] 马秋霞, 刘楠, 丛黎明, 等. 改进的FilmplateTM纸片法与国标法检测大肠菌群数的比较[J]. 解放军预防医学杂志, 2015, 33(1):14-17.
MA Q X, LIU N, CONG L M, et al. Comparison between modified filmplateTM method and national standard method for the detection of coliform groups[J]. Journal of Preventive Medicine of Chinese PLA, 2015, 33(1):14-17.
[18] 蔡金华, 刘雅妮, 顾欣. 链霉素在牛奶中残留的微生物学检测方法[J]. 中国兽药杂志, 2004, 38(11):7-9.
CAI J H, LIU Y N, GU X. Determination of streptomycin residues in milkby microbiologic inhibition tests[J]. Chinese Journal of Veterinary Drug, 2004, 38(11):7-9.
[19] WEN Y P, LIAO X N, DENG C X, et al. Imprinted voltammetric streptomycin sensor based on a glassy carbon electrode modified with electropolymerized poly(pyrrole-3-carboxy acid) and electrochemically reduced graphene oxide[J]. Microchimica Acta, 2017, 184(3):935-941.
[20] 温馨, 刘静, 赵斯琴, 等. GO/介孔TiO2复合材料的制备及性能研究[J]. 化学试剂, 2018, 40(7):623-628.
WEN X, LIU J, ZHAO S Q, et al. Preparation and properties of GO/TiO2 mesoporous composites[J]. Chemical Reagents, 2018, 40(7):623-628.
[21] 李荣强, 张军丽, 闫永康. GO-CMWNTs-CS-GCE电极检测腐败小白菜中亚硝酸盐的含量[J]. 化学试剂, 2018, 40(3):235-238.
LI R Q, ZHANG J L, YAN Y K. Electrochemical detection of nitrite content in corrupt Chinese cabbage by GO-CMWNTs-CS-GCE electrode[J]. Chemical Reagents, 2018, 40(3):235-238.
[22] CHOKKAREDDY R, REDHI G G, THANGAVEL K. Cytochrome c/multi-walled carbon nanotubes modified glassy carbon electrode for the detection of streptomycin in pharmaceutical samples[J]. Analytical Sciences: the International Journal of the Japan Society for Analytical Chemistry, 2021, 37(9):1265-1273.
[23] HU Y F, ZHANG Z H, ZHANG H B, et al. Selective and sensitive molecularly imprinted Sol-gel film-based electrochemical sensor combining mercaptoacetic acid-modified PbS nanoparticles with Fe3O4@Au-multi-walled carbon nanotubes-chitosan[J]. Journal of Solid State Electrochemistry, 2012, 16(3):857-867.
[24] LIU B Q, TANG D P, ZHANG B, et al. Au(III)-promoted magnetic molecularly imprinted polymer nanospheres for electrochemical determination of streptomycin residues in food[J]. Biosensors & Bioelectronics, 2013, 41:551-556.
[25] QUE X H, LIU B Q, FU L B, et al. Molecular imprint for electrochemical detection of streptomycin residues using enzyme signal amplification[J]. Electroanalysis, 2013, 25(2):531-537.
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