食品与发酵工业 >

2024 , Vol. 50 >Issue 18: 98 - 106

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.039706

研究报告

高温产2,3-丁二醇甲基芽孢杆菌的筛选及其发酵优化

  • 林燕环 ,
  • 陈飞雪 ,
  • 魏宸鑫 ,
  • 李润志 ,
  • 张燎原 ,
  • 林辉
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  • 1(漳州职业技术学院 食品工程学院,福建 漳州,363000)
    2(福建农林大学 生命科学学院,福建 福州,350002)
    3(福建省农业科学院 食用菌研究所,福建 福州,350013)
第一作者：硕士研究生(林辉助理研究员为通信作者,E-mail:lh4832@126.com)

收稿日期: 2024-04-26

  修回日期: 2024-06-05

  网络出版日期: 2024-10-14

基金资助

国家自然科学基金项目(81673542);福建省自然科学基金项目(2023J05067)

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Screening of thermophilic Bacillus methylotrophicus for efficient 2,3-butanediol production and its fermentation optimization

  • LIN Yanhuan ,
  • CHEN Feixue ,
  • WEI Chenxin ,
  • LI Runzhi ,
  • ZHANG Liaoyuan ,
  • LIN Hui
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  • 1(College of Food Engineering, Zhangzhou Institute of Technology, Zhangzhou 363000, China)
    2(College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China)
    3(Institute of Edible Fungi, Fujian Academy of Agricultural Sciences, Fuzhou 350013, China)

Received date: 2024-04-26

  Revised date: 2024-06-05

  Online published: 2024-10-14

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摘要

2,3-丁二醇是一种典型的大宗化学品,广泛应用于化工、食品、医药、燃料等领域。生物法发酵制备2,3-丁二醇是当下的主要方法,然而,大多数报道的菌株为条件致病菌且发酵条件严格,限制了生物法制备2,3-丁二醇的发展。该研究以温泉底土壤为试验材料,经过分离、筛选与鉴定获得了1株高温条件下产2,3-丁二醇的甲基芽孢杆菌。通过摇瓶实验优化发酵工艺,确定最适发酵条件与最优培养基组成,利用优化后的发酵工艺甲基芽孢杆菌发酵24 h产物产量达到32.57 g/L。进一步进行上罐试验,基于不同发酵罐转速条件下甲基芽孢杆菌的产量积累情况,理性设计两阶段发酵策略,并以最优策略分批补料发酵制备2,3-丁二醇,2,3-丁二醇的产量在甲基芽孢杆菌发酵45 h时达到73.19 g/L,转化率达到90.25%。

关键词： 2,3-丁二醇; 甲基芽孢杆菌; 高温发酵; 发酵优化; 分批补料发酵

本文引用格式

林燕环 , 陈飞雪 , 魏宸鑫 , 李润志 , 张燎原 , 林辉 . 高温产2,3-丁二醇甲基芽孢杆菌的筛选及其发酵优化[J]. 食品与发酵工业, 2024 , 50(18) : 98 -106 . DOI: 10.13995/j.cnki.11-1802/ts.039706

Abstract

2,3-Butanediol, an important bulk chemical, is widely used in the field of chemical industry, food, pharmaceutical and fuel.Biological 2,3-butanediol production via microbial fermentation is the main method at present.However, several reported strains with high yields are opportunistic pathogen, and strict fermentation conditions are required, which limited the progress of biological 2,3-butanediol production.In this study, a newly isolated Bacillus methylotrophicus strain that thermophilically produces 2,3-butanediol was screened and identified from the soil of hot spring.The optimized fermentation conditions and medium components resulted in 2,3-butanediol production with 32.57 g/L at 24 h.Furthermore, the fermentation experiment in a 5 L bioreactor was performed, and the effects of different agitation speeds on 2,3-butanediol production were investigated.Ultimately, a two-stage fermentation strategy combined with fed-batch was developed, and 73.19 g/L 2,3-butanediol with the yield of 90.25% was achieved in a 5 L bioreactor at 45 h.

Key words： 2,3-butanediol; Bacillus methylotrophicus; thermophilic fermentation; fermentation optimization; fed-batch fermentation

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