该研究旨在探索鼠李糖乳酪杆菌AFY05在小鼠急性痛风性关节炎中的作用机制。采用尿酸钠盐建立了小鼠急性痛风性关节炎模型,并通过观察小鼠的足肿胀程度、痛阈值、血液生化指标、组织病理学变化和mRNA表达变化来评估AFY05在预防小鼠急性痛风性关节炎中的作用和机制。动物实验结果显示,AFY05能减轻小鼠足肿胀程度并提高痛阈值。AFY05可提高急性关节炎小鼠踝关节组织中超氧化物歧化酶(superoxide dismutase,SOD)的酶活力和谷胱甘肽(glutathione,GSH)水平,同时降低髓过氧物酶(myeloperoxidase,MPO)的酶活力和丙二醛(malondialdehyde,MDA)的水平。AFY05还减轻了急性关节炎小鼠血清中IL-6、IL-10、IL-1β和TNF-α的细胞因子水平。AFY05还能减轻急性关节炎小鼠血清中的IL-6、IL-10、IL-1β、TNF-α细胞因子水平。组织病理学观察显示AFY05减轻了小鼠足踝关节的组织损伤。qPCR实验结果发现AFY05能够下调急性关节炎小鼠踝关节组织中的细胞外调节激酶1/2(extracellular signal-regulated kinase 1/2,ERK1/2)、环氧合酶-2(cyclooxygenase-2,COX-2)、前列腺素E2(prostaglandin E2,PGE2)、IL-6、干扰素-γ(interferon γ,IFN-γ)、TNF-α的mRNA表达和上调铜/锌超氧化物歧化酶(copper/zinc superoxide dismutase,Cu/Zn-SOD)、锰超氧化物歧化酶(manganese superoxide dismutase,Mn-SOD)、过氧化氢酶(catalase,CAT)的mRNA表达。由此可见,鼠李糖乳酪杆菌AFY05能够预防急性痛风性关节炎,且效果优于硫酸氨基葡萄糖,是一株具备益生菌潜质的菌株。
The objective of this study is to investigate the mechanism of action of Lactobacillus rhamnosus AFY05 in the context of acute gouty arthritis in mice.To achieve this, a mouse model of acute gouty arthritis was established using sodium urate.The effect and mechanism of AFY05 in preventing acute gouty arthritis in mice were then evaluated by assessing foot swelling, pain threshold, blood biochemical indicators, histopathological changes, and mRNA expression changes.The findings from the animal experiment revealed that AFY05 reduced foot swelling and increased pain threshold in mice.Moreover, AFY05 enhanced the enzyme activity of superoxide dismutase (SOD) and the level of glutathione (GSH) in the ankle joint tissues of mice with acute arthritis, while reducing the enzyme activity of myeloperoxidase (MPO) and the level of malondialdehyde (MDA).Additionally, AFY05 decreased the levels of interleukin-6 (IL-6), IL-10, IL-1β, and tumor necrosis factor-alpha (TNF-α) in the serum of mice with acute arthritis.Histopathological observations indicated that AFY05 alleviated tissue damage in the ankle joints of mice.Results from the qPCR experiment demonstrated that AFY05 downregulated the mRNA expressions of extracellular signal-regulated kinase 1/2 (ERK1/2), cyclooxygenase-2 (COX-2), prostaglandin E2 (PGE2), IL-6, interferon gamma (IFN-γ), and TNF-α in the ankle joint tissues of mice with acute arthritis.Furthermore, AFY05 upregulated the expressions of copper/zinc superoxide dismutase (Cu/Zn-SOD), manganese superoxide dismutase (Mn-SOD), and catalase (CAT).These findings suggest that Lactobacillus rhamnosus AFY05 exhibits potential in preventing acute gouty arthritis, surpassing the efficacy of glucosamine sulfate.Thus, it can be regarded as a strain with probiotic potential.
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