食品与发酵工业 >

2024 , Vol. 50 >Issue 18: 326 - 331

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.037596

分析与检测

枇杷中氟吡菌酰胺、肟菌酯及代谢产物残留分析与膳食风险评估

  • 王思威 ,
  • 王潇楠 ,
  • 常虹 ,
  • 刘艳萍
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  • (广东省农业科学院植物保护研究所,农业农村部华南果蔬绿色防控重点实验室,广东省植物保护新技术重点实验室,广东 广州,510640)
第一作者：博士,副研究员(刘艳萍研究员为通信作者,E-mail:liuliuyp@tom.com)

收稿日期: 2023-10-09

  修回日期: 2023-12-28

  网络出版日期: 2024-10-14

基金资助

2021年省级乡村振兴战略专项(粤财农[2021]37号)

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Distribution and risk assessment of fluopyram and trifloxystrobin residue in loquat

  • WANG Siwei ,
  • WANG Xiaonan ,
  • CHANG Hong ,
  • LIU Yanping
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  • (Institute of Plant Protection, Guangdong Academy of Agricultural Sciences, Key Laboratory of Green Prevention and Control on Fruits and Vegetables in South China Ministry of Agriculture and Rural Affairs, Guangdong Provincial Key Laboratory of High Technology for Plant Protection, Guangzhou 510640, China)

Received date: 2023-10-09

  Revised date: 2023-12-28

  Online published: 2024-10-14

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摘要

为明确氟吡菌酰胺和肟菌酯在枇杷上的残留与膳食风险,采用超高效液相色谱-串联质谱仪(ultra-performance liquid chromatography- tandem mass spectrometry,UPLC-MS/MS)检测了枇杷中氟吡菌酰胺及其代谢物2-(三氟甲基)苯甲酰胺和肟菌酯及其代谢物肟菌酸在果实生长过程中的消解动态和残留量,并进行了膳食风险评估。样品采用乙腈提取,氨基固相萃取柱净化,UPLC-MS/MS检测。结果表明,在0.01～1.00 mg/kg添加水平下,回收率为82%~93%,相对标准偏差为1.4%～2.8%,定量限均为0.01 mg/kg,方法灵敏度及准确度良好,能够满足枇杷中农药残留检测要求。按照良好农业规范要求,在枇杷上施用43%氟吡菌酰胺·肟菌酯悬浮剂,当剂量为333 mg/kg、施药2次,施药间隔10~15 d,距末次施药后28、35 d时,枇杷中氟吡菌酰胺残留量为<0.01～0.03 mg/kg、消解率>90%;肟菌酯的残留量为<0.02～0.04 mg/kg、消解率>85%,肟菌酯的残留量低于我国规定的最大残留限量0.7 mg/kg。膳食风险评估表明,按照推荐剂量使用,枇杷中氟吡菌酰胺残留水平对一般人群健康不会产生不可接受的风险。研究结果可为氟吡菌酰胺和肟菌酯在枇杷树上的科学合理使用提供数据和技术支撑。

关键词： 枇杷; 氟吡菌酰胺; 肟菌酯; 残留分析; 膳食风险评估

本文引用格式

王思威 , 王潇楠 , 常虹 , 刘艳萍 . 枇杷中氟吡菌酰胺、肟菌酯及代谢产物残留分析与膳食风险评估[J]. 食品与发酵工业, 2024 , 50(18) : 326 -331 . DOI: 10.13995/j.cnki.11-1802/ts.037596

Abstract

In this experiment, the dissipation dynamics and residue distribution of fluopyram, trifloxystrobin, and their metabolites [2-(trifluoromethyl) benzamide (BZM) and trifloxystrobin acid] during the growth process of loquat were carried out by ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS).Samples were extracted with acetonitrile, purified with an amino solid phase extraction column, and analyzed by UPLC-MS/MS.Results showed that at three spiked levels of 0.01-1 mg/kg, the average recoveries were 82%-93%, the relative standard deviation (RSD) was 1.4%-2.8%, and the limit of quantification (LOQ) was 0.01 mg/kg.The method had good sensitivity and accuracy and could meet the requirements for residue detection in loquat.According to the guidelines for field trials of pesticide residues, the field trial results showed that when fluopyram · trifloxystrobin 43% suspension concentrate (SC) was applied at dosage of 333 mg/kg for 2 times with application interval of 10-15 days, and 28 and 35 days after the last application, the residues and dissipation rate of fluopyram and trifloxystrobin was <0.01-0.03 mg/kg and >90%, <0.02-0.04 mg/kg and >85%, respectively.The residue of trifloxystrobin was lower than the maximum residue limit formulated in China (0.7 mg/kg).The dietary risk assessment results showed that it did not pose a chronic risk to the general population according to the recommended dosage.The research results can provide data and technical support for guiding the scientific and rational use of fluopyram and trifloxystrobin in loquat.

Key words： loquat; fluopyram; trifloxystrobin; residue analysis; dietary risk assessment

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