食品与发酵工业 >

2024 , Vol. 50 >Issue 20: 209 - 215

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.037822

研究报告

基于BDNF/TrkB/CREB信号通路探讨核桃油对东莨菪碱导致认知障碍大鼠的保护作用

  • 尤佳伟 ,
  • 孙亚凤 ,
  • 夏君霞 ,
  • 李奕阳 ,
  • 刘媛 ,
  • 杨慧 ,
  • 齐兵 ,
  • 戴胜兴 ,
  • 吴彦兵 ,
  • 田粟
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  • 1(河北医科大学 公共卫生学院营养与食品卫生学教研室,河北 石家庄,050017)
    2(河北省环境与人群健康重点实验室,河北 石家庄,050017)
    3(河北养元智汇饮品股份有限公司,河北 衡水,053000)
    4(河北省核桃营养功能与加工技术重点实验室,河北 衡水,053000)
    5(河北省核桃饮品技术创新中心,河北 衡水,053000)
第一作者:硕士研究生(田粟副教授为通信作者,E-mail:sutian314@hebmu.edu.cn)

收稿日期: 2023-11-01

  修回日期: 2023-12-06

  网络出版日期: 2024-11-01

基金资助

河北省创新能力提升计划项目(22567667H)

收起

Effects of walnut oil on scopolamine-induced cognitive impairment in rats based on BDNF/TrkB/CREB pathway

  • YOU Jiawei ,
  • SUN Yafeng ,
  • XIA Junxia ,
  • LI Yiyang ,
  • LIU Yuan ,
  • YANG Hui ,
  • QI Bing ,
  • DAI Shengxing ,
  • WU Yanbing ,
  • TIAN Su
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  • 1(Department of Nutrition and Food Hygiene, School of Public Health, Hebei Medical University, Shijiazhuang 050017, China)
    2(Hebei Key Laboratory of Environment and Human Health, Shijiazhuang 050017, China)
    3(Hebei Yangyuan Zhihui Beverage Co.Ltd., Hengshui 053000, China)
    4(Hebei Key Laboratory of Walnut Nutritional Function and Processing Technology, Hengshui 053000, China)
    5(Hebei Technology Innovation Centre of Walnut Beverage, Hengshui 053000, China)

Received date: 2023-11-01

  Revised date: 2023-12-06

  Online published: 2024-11-01

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摘要

研究显示,膳食营养在改善认知功能衰退、降低痴呆风险方面发挥着重要作用。该研究基于脑源性神经营养因子(brain-derived neurotrophic factor, BDNF)/原肌球蛋白相关激酶B(tropomyosin-related kinase B, TrkB)/环磷腺苷效应元件结合蛋白(cAMP-response element binding protein, CREB)信号通路探讨核桃油对东莨菪碱致认知障碍大鼠的保护作用。将24只SD大鼠随机分为空白对照组、模型对照组、核桃油组。先进行30 d的核桃油灌胃,其他2组给予等体积生理盐水处理。第31天开始模型对照组和核桃油组每日腹腔注射3 mg/(kg·d)东莨菪碱,通过新物体识别和Morris水迷宫测试记忆能力,为期5 d。苏木精-伊红染色法(hematoxylin-eosin staining,HE)染色观察海马组织形态,试剂盒检测脑组织乙酰胆碱(acetylcholine, ACh)含量和乙酰胆碱酯酶(acetylcholinesterase, AChE)活性,蛋白质免疫印迹法测定海马组织突触素(synaptophysin, SYN)、突触后致密蛋白-95(postsynaptic density-95, PSD-95)、BDNF、TrkB、CREB的表达。结果显示,与模型对照组比较,核桃油组的学习记忆能力显著升高;神经元细胞排列变得整齐、结构趋向完整;海马组织的ACh含量增加,AChE活性降低;海马组织中目的蛋白表达均显著升高,提示核桃油对东莨菪碱诱导的认知障碍具有保护作用,其可能机制与激活BDNF/TrkB/CREB信号通路调节突触可塑性有关。

关键词： 核桃油; 东莨菪碱; 认知障碍; BDNF/TrkB/CREB信号通路; 突触可塑性

本文引用格式

尤佳伟 , 孙亚凤 , 夏君霞 , 李奕阳 , 刘媛 , 杨慧 , 齐兵 , 戴胜兴 , 吴彦兵 , 田粟 . 基于BDNF/TrkB/CREB信号通路探讨核桃油对东莨菪碱导致认知障碍大鼠的保护作用[J]. 食品与发酵工业, 2024 , 50(20) : 209 -215 . DOI: 10.13995/j.cnki.11-1802/ts.037822

Abstract

Research has shown that nutrition and diet play a key role in improving cognitive decline and reducing the risk of dementia.This study aimed to investigate the effect of walnut oil on scopolamine-induced cognitive impairment in rats based on the BDNF/TrkB/ CREB signaling pathway.24 SD rats were randomly divided into a control group, a model group, and a walnut oil group.First, walnut oil was gavaged for 30 days, and the other two groups were treated with equal volume of saline.On the 31 st day, the model group and walnut oil group were intraperitoneally injected with 3 mg/(kg·d) of scopolamine daily for 5 days.The learning and memory abilities were tested through new object recognition experiments and Morris water maze tests.HE staining was used to observe the pathological morphology of the hippocampus.The acetylcholine (ACh) content and acetylcholinesterase (AChE) activity in the brain tissue were detected using a reagent kit.Western blot was used to detect synaptophysin (SYN), postsynaptic density-95 (PSD-95), brain-derived neurotrophic factor (BDNF), tropomyosin-related kinase B (TrkB), and cAMP-response element binding protein (CREB) expression levels in the hippocampus.It found that walnut oil could improve the learning and memory ability of rats in the scopolamine-induced cognitive impairment model, as well as neuronal cell arrangement, and structural integrity.It could also decrease the activity of AChE, increase the level of ACh, and significantly elevate the expression of target proteins in hippocampal tissues.This suggests that walnut oil has an effect on cognitive function in scopolamine-induced cognitive impairment and its possible mechanism is related to the activation of the BDNF/TrkB/CREB signaling pathway to regulate synaptic plasticity.

Key words： walnut oil; scopolamine; cognitive impairment; BDNF/TrkB/CREB signal pathway; synaptic plasticity

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