食品与发酵工业 >

2024 , Vol. 50 >Issue 20: 303 - 312

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.039887

分析与检测

基于超高效液相色谱-串联质谱技术对葡萄籽提取物化学成分的分析及其多成分含量测定

  • 李进发 ,
  • 刁娟娟 ,
  • 陈章浩 ,
  • 高霜 ,
  • 常军民
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  • 1(新疆医科大学 药学院,新疆 乌鲁木齐,830011)
    2(新疆医科大学中心实验室,新疆 乌鲁木齐,830011)
    3(新疆天然药物活性组分与释药技术重点实验室,新疆 乌鲁木齐,830011)
第一作者:硕士研究生(常军民教授为通信作者,E-mail:1617265908@qq.com)

收稿日期: 2024-05-15

  修回日期: 2024-07-15

  网络出版日期: 2024-11-01

基金资助

新疆维吾尔自治区重大科技专项项目(2022A03019-3)

收起

Chemical composition analysis and multi-component content determination of grape seed extract based on ultra performance liquid chromatography tandem mass spectrometry

  • LI Jinfa ,
  • DIAO Juanjuan ,
  • CHEN Zhanghao ,
  • GAO Shuang ,
  • CHANG Junmin
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  • 1(School of Pharmacy, Xinjiang Medical University, Urumqi 830011, China)
    2(Central Laboratory, Xinjiang Medical University, Urumqi 830011,China)
    3(Xinjiang Key Laboratory of Natural Drug Active Components and Drug Release Technology, Urumqi 830011, China)

Received date: 2024-05-15

  Revised date: 2024-07-15

  Online published: 2024-11-01

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摘要

该研究应用液质联用技术结合UNIFI筛查平台,对葡萄籽提取物中化学成分进行快速定性分析,并建立葡萄籽提取物中有效成分的含量测定方法。首先利用超高效液相色谱-四级杆-飞行时间高分辨质谱技术采集葡萄籽提取物的质谱数据,根据UNIFI软件自建数据库匹配的结果,结合对照品保留时间、特征碎片离子及相关文献确认,从葡萄籽提取物中共鉴别出20个化学成分,包括7个多酚类、1个三萜类,1个黄酮醇类、1个不饱和脂肪酸类、1个多羟基茋类、3个酚酸类、6个氨基酸类。另外,采用超高效液相色谱三重四极杆质谱法同时定量测定了葡萄籽提取物中4种有效成分(儿茶素、表儿茶素、原花青素B2、原花青素C1),其在各自范围内线性关系良好(r≥0.998),平均加样回收率为96.7%～102.6%,RSD为1.6%～3.1%。该研究结果为葡萄籽提取物中化学成分的定性及定量分析提供了一种快速、简便、准确的方法,为其质量评价及药效物质基础研究提供了重要的理论参考。

关键词： 葡萄籽提取物; 化学成分分析; 含量测定; 超高效液相色谱-串联质谱

本文引用格式

李进发 , 刁娟娟 , 陈章浩 , 高霜 , 常军民 . 基于超高效液相色谱-串联质谱技术对葡萄籽提取物化学成分的分析及其多成分含量测定[J]. 食品与发酵工业, 2024 , 50(20) : 303 -312 . DOI: 10.13995/j.cnki.11-1802/ts.039887

Abstract

In this study, liquid chromatography-mass spectrometry (LC-MS) combined with the UNIFI screening platform was used to quickly and qualitatively analyze the chemical components in grape seed extract, and establish a method for the determination of effective components in grape seed extract.Firstly, the mass spectrum data of grape seed extract were collected by ultra-performance liquid chromatography-quadrupole-time-of-flight high-resolution mass spectrometry (UPLC-Q-TOF-MS/MS).According to the matching results of the database built by UNIFI software, combined with the retention time of control substance, characteristic fragment ions and related literature, 20 chemical constituents were identified from grape seed extract, including 7 polyphenols, 1 triterpene, 1 flavonol, 1 unsaturated fatty acid, 1 polyhydroxylamine, 3 phenolic acids, and 6 amino acids.In addition, the four active components (catechin, epicatechin, proanthocyanidin B2 and proanthocyanidin C1) in grape seed extract were determined simultaneously by ultra performance liquid chromatography-triple quadrupole mass spectrometry (UPLC-QqQ-MS/MS).The linear relationship was good in their respective ranges (r≥0.998), and the average sample recovery was 96.7%-102.6%.This study provides a rapid, simple, and accurate method for qualitative and quantitative analysis of chemical components in grape seed extract, providing an important theoretical reference for its quality evaluation and basic research of pharmacodynamic substances.

Key words： grape seed extract; chemical composition analysis; content determination; ultra performance liquid chromatography tandem mass spectrometry

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