为了获得红酸汤发酵专用植物乳杆菌NCU001929(Lactobacillus plantarum NCU001929)的高密度培养方法,该文考察了培养基成分、静态培养条件和发酵罐工艺条件对菌体活菌数的影响。结果表明,优化后最佳的培养基成分为麦芽糖34.35 g/L、牛肉浸粉17.40 g/L,酵母提取粉18.62 g/L,柠檬酸三铵2.80 g/L,细菌学蛋白胨10.00 g/L,MnSO4·H2O 0.06 g/L,无水乙酸钠3.00 g/L,维生素C 0.02 g/L,吐温-80 1.00 mL/L,K2HPO4 2.00 g/L;静态培养最佳条件为接种量2%(体积分数),pH值为7,温度30 ℃,此时活菌数为6.40×109 CFU/mL,是MRS培养基的4.81倍;发酵罐最佳工艺条件为转速150 r/min,通入无菌空气,维持发酵罐pH值为7.0,以25%(质量分数)的NaOH作为中和剂,活菌数达1.32×1010 CFU/mL,是MRS培养基的9.92倍。综上所述,该研究建立了一种红酸汤直投式剂的制备方法,为贵州红酸汤工业化生产提供了技术支持。
In order to achieve the high density culture conditions of L.plantarum NCU001929, the optimum culture conditions were determined through the experiments of medium composition, static culture and fermenter technology.The optimal medium for liquid fermentation of L.plantarum NCU001929 contained maltose (34.35 g/L), beef extract (17.40 g/L), yeast extract (18.62 g/L), triammonium citrate (2.80 g/L), bacteriological peptone (10.00 g/L), MnSO4·H2O (0.06 g/L), anhydrous sodium acetate (3.00 g/L), vitamin C (0.02 g/L), Tween-80 (1 mL/L), and dipotassium hydrogen phosphate (2.0 g/L).Suitable culture conditions were 2% inoculation amount, pH 7 and the culture temperature 30 ℃, with the viable bacteria number of 6.40×109 CFU/mL, which is 4.81 times that of initial MRS medium.Additionally the optimal process parameters for fermentation tanks were stir speed 150 r/min,incubate with sterile air, constant pH of 7 and neuralizing agent 25% NaOH, with the viable bacteria number of 1.32×1010 CFU/mL, which is 9.92 times that of initial MRS medium.In conclusion, a preparation method of red sour soup direct vet set was established, which provides a technical support for the industrial production of Guizhou red sour soup.
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