Camellia sinensis var.assamica is a significant variety in China, and its composition impacts the regulation of intestinal microecology, sugar, lipid metabolism, and other functions.This study comparatively analyzed the influence of white tea, Pu'er raw tea, Pu'er ripe tea, and sour tea on the intestinal microecology of varied populations, prepared through different processes.An in vitro model for intestinal microbiota digestion was constructed by using fecal samples from healthy individuals as well as those suffering from constipation.Following co-culturing with tea soup for 24 hours, the fermentation broth was analyzed to determine both the microbial composition and short-chain fatty acid content.Additionally, metabolic pathway prediction with PICRUSt was performed.In both models, Camellia sinensis var.assamica decreased the ratio of Firmicutes/Bacteroidetes and promoted the abundance of Ruminococcus, Lachnospira, Bacteroidetes, Butyricicoccus, Akkermansia muciniphila, and other butyric acid-producing bacteria.It also inhibited pathogenic bacteria such as Streptococcus and Staphylococcus while increasing the content of short-chain fatty acids.Technical term abbreviations have been explained when first used.In the standard model, notable interventions were noted in metabolic pathways such as digestion, energy and lipid metabolism, biodegradation of exogenous compounds, and intestinal inflammation protection.In contrast, in the constipation model, Pu'er ripe tea significantly impacted only two metabolic pathways linked to cardiovascular health and the circulatory system.Compared to other Camellia sinensis var.assamica, Pu'er ripe tea considerably enhanced the diversity of intestinal flora.Additionally, sour tea notably increased the content of short-chain fatty acids in the constipation model.
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