该研究考察黄大茶、薏苡仁和茯苓复配物对高脂饮食诱导肥胖改善的作用与机制。采用高脂饮食喂养C57/BL 6 N雄性小鼠12周,同时分别用黄大茶复配物(large-leaf yellow tea formula aqueous extracts,LFAE)、薏苡仁+茯苓水提物(Coix lachrymajobi L.combined with Poria cocos Wolf formula aqueous extracts, CPAE)或黄大茶水提物(large-leaf yellow tea aqueous extracts,LAE)干预处理,每周记录小鼠的体重及能量摄入。饮食干预完成后,检测各组小鼠血脂水平变化,HE染色法检测小鼠肝脏及附睾脂肪组织(epididymal adipose tissue,EAT)的形态变化,采用16S rRNA高通量测序分析各组小鼠肠道菌群的多样性与差异性,Western blotting检测EAT中腺苷酸活化蛋白激酶(AMP-activated protein kinase,AMPK)、p-AMPK、甘油三酯脂肪酶(adipose triglyceride lipase,ATGL)、激素敏感性脂肪酶(hormone-sensitive lipase,HSL)和p-HSL蛋白的表达。结果显示,与高脂组相比,CPAE和LAE组小鼠体重降低均不显著,而LFAE组小鼠的体重、TC、LDL-C、AST、ALT水平和脂质沉积显著降低,肠道菌群中厚壁菌门丰度显著降低,拟杆菌门丰度升高,属水平中阿德勒克罗伊茨菌(Adlercreutzia)、异杆菌属(Allobaculum)、经黏液真杆菌属(Blautia)、狄氏副拟杆菌(Parabacteroides)丰度显著提高,p-AMPK、ATGL和p-HSL蛋白表达均显著上调。此外,与LAE组相比,LFAE组小鼠的体重、TG水平和脂质沉积显著降低,厚壁菌门丰度降低,拟杆菌门丰度升高,Adlercreutzia、Allobaculum、Blautia、Parabacteroides丰度显著提高,ATGL和p-HSL蛋白的表达均显著上调。综上所述,黄大茶、薏苡仁和茯苓复配物能够调节小鼠肠道菌群,促进脂肪组织脂解,协同增效改善高脂饮食诱导的肥胖。
This study aimed to investigate the effects and mechanism of the combined formula (large-leaf yellow tea, Coix lachrymajobi L., Poria cocos Wolf) aqueous extracts.C57/BL 6 N male mice were fed on a high-fat diet (HFD), HFD with large-leaf yellow tea formula aqueous extracts (LFAE), or HFD with Coix lachrymajobi L.combined with Poria cocos Wolf formula aqueous extracts (CPAE), or HFD with large-leaf yellow tea aqueous extracts (LAE), and the body weight and food intake of mice were measured weekly.After a 12-week treatment, the serum levels of lipids were detected.The lipid deposition in the liver and epididymal adipose tissue (EAT) was visualized by hematoxylin-eosin (HE) staining.The abundance of gut microbiota was analyzed by high-throughput 16S rRNA gene amplification.The protein expressions of AMPK, p-AMPK, ATGL, HSL, and p-HSL in EAT were analyzed by Western blotting.Compared with the HFD-fed group, neither the CPAE nor LAE group showed a significant reduction in body weight.The body weight, lipid levels, and lipid accumulation were significantly reduced in the LFAE group.Moreover, LFAE treatment significantly decreased the abundance of Firmicutes and increased the abundance of Bacteroidetes, Adlercreutzia, Allobaculum, Blautia, and Parabacteroides.The protein expressions of p-AMPK, ATGL, and p-HSL were up-regulated in the LFAE group.In addition, compared with the LAE group, the body weight, TG levels, and lipid accumulation were significantly reduced in the LFAE group.The abundance of Firmicutes was decreased, and the abundance of Bacteroidetes, Adlercreutzia, Allobaculum, Blautia, and Parabacteroides was up-regulated.Meanwhile, the protein expressions of ATGL, and p-HSL were significantly up-regulated.In conclusion, these results indicated that LFAE synergistically ameliorated diet-induced obesity and related metabolic disorders, which may be through increasing the diversity of gut microbiota and the abundance of lipid metabolism-related bacteria and promoting lipolysis of epididymal adipose tissue in mice.
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