This study aimed to explore the effect of Saccharomyces cerevisiae fermentation on the structural characteristics and antioxidant activity of corn silk polysaccharides.Fermented corn silk polysaccharides (FCSP) were prepared by fermentation of Saccharomyces cerevisiae.The content of polysaccharide in FCSP and water extracted corn silk polysaccharide (WCSP) was detected by the phenol sulfuric acid method, and the protein content was analyzed by the Dumas combustion method.The relative molecular weight and monosaccharide composition were determined by HPLC.The antioxidant activity differences between FCSP and WCSP in vitro were compared and the oxidative stress model of zebrafish embryos was induced by 5 mmol/L H2O2 to further explore the antioxidant activity of FCSP in vivo.The optimal fermentation condition was as follows:liquid fermentation of 30 ℃, shaking 2 days (130 r/min), inoculation rate of 1%, initial pH 6, glucose addition amount of 1.0%.Under these conditions, the highest polysaccharides yield was (1.40±0.01)%, which was 18.64% higher than the water extraction method (1.18±0.01)%.However, there was no significant change in the relative molecular weight of FCSP and WCSP.The monosaccharide composition of FCSP was mainly composed of glucose, mannose, rhamnose, galactose, xylose, and arabinose, compared with WCSP, the molar ratio of mannose, rhamnose, and glucose increased, while the contents of galacturonic acid, galactose, and arabinose decreased significantly.The results of antioxidant activity in vitro showed that the IC50 values of FCSP for ·OH radicals, DPPH radicals, and ABTS cationical radicals were 4.42 mg/mL, 59.30 μg/mL, and 12.43 μg/mL, respectively, which were 10.86%, 11.61%, and 22.26% lower than those of WCSP, indicating that FCSP had stronger antioxidant capacity in vitro.The results of in vivo antioxidant activity in vivo showed that FCSP could significantly reduce the mortality and heart rate of zebrafish in the model group, alleviate the morphological and developmental abnormalities caused by oxidative stress, restore the vitality of zebrafish in the model group, and improve its response ability under light and dark stimulation, indicating that FCSP had a significant protective effect on zebrafish with oxidative stress induced by H2O2.
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