研究报告

霉菌发酵剂的制备及其对干腌肉块品质的影响

  • 臧立萍 ,
  • 魏建平 ,
  • 何厚德 ,
  • 向俊 ,
  • 陈言杰 ,
  • 赵晓霞 ,
  • 汪雪娇 ,
  • 曹建新
展开
  • 1(昆明理工大学 食品科学与工程学院,云南 昆明,650500)
    2(西北大学 食品科学与工程学院,陕西 西安,710069)
    3(昆明德和罐头食品有限责任公司,云南 昆明,650500)
第一作者:硕士研究生(汪雪娇讲师和曹建新教授为共同通信作者,E-mail:wangxuejiao173@hotmail.com;jxcao321@hotmail.com)

收稿日期: 2023-12-22

  修回日期: 2024-01-10

  网络出版日期: 2024-11-28

基金资助

云南省重大科技项目(202202AG050009);云南省“兴滇英才支持计划”青年人才项目(KKRD202246038);国家自然科学基金项目(32001673)

Preparation of mold starter cultures and its effect on the quality of dry-cured pork

  • ZANG Liping ,
  • WEI Jianping ,
  • HE Houde ,
  • XIANG Jun ,
  • CHEN Yanjie ,
  • ZHAO Xiaoxia ,
  • WANG Xuejiao ,
  • CAO Jianxin
Expand
  • 1(Faculty of Food Science and Engineering, Kunming University of Science and Technology, Kunming 650500, China)
    2(College of Food Science and Technology, Northwest University, Xi'an 710069, China)
    3(Teh-ho Canned-Food Company, Kunming 650500, China)

Received date: 2023-12-22

  Revised date: 2024-01-10

  Online published: 2024-11-28

摘要

以干腌肉块作为宣威火腿的模拟体系,探讨6组霉菌发酵辅助剂对其物理化学特性和风味品质的影响。在6组发酵辅助剂中,鲁本斯青霉W10-1+褐藻青霉V8展现出了最高的中性、酸性蛋白酶和脂肪酶酶活性。将发酵辅助剂接种至干腌肉块中,发现相较于自然发酵组,接种霉菌均能提高干腌肉块的水分活度、pH、蛋白降解指数,且能使其维持在干腌肉制品的最佳范围内;此外,能增加挥发性风味化合物的种类并降低醛类物质的含量,改善亮度和红度,降低剪切力和硫代巴比妥酸值。其中,W10-1+V8组表现最为优异。电子鼻分析表明,W10-1+V8组与自然发酵组的风味特性最为接近,还能缓解脂质过度氧化。电子舌分析表明,W10-1+V8组在鲜味、丰富度上显著高于自然发酵组,酸味显著低于自然发酵组。因此,鲁本斯青霉W10-1+褐藻青霉V8最适宜作为干腌肉制品的发酵剂,为提升宣威火腿的品质提供技术支撑。

本文引用格式

臧立萍 , 魏建平 , 何厚德 , 向俊 , 陈言杰 , 赵晓霞 , 汪雪娇 , 曹建新 . 霉菌发酵剂的制备及其对干腌肉块品质的影响[J]. 食品与发酵工业, 2024 , 50(21) : 144 -153 . DOI: 10.13995/j.cnki.11-1802/ts.038345

Abstract

The effects of six mold starter cultures on the physicochemical properties and flavor of dry-cured pork, as the simulation system of Xuanwei hams, were investigated.Among the six mold starter cultures, the Penicillium rubens W10-1+Penicillium fuscoglaucum V8 group exhibited the highest neutral/acid protease and lipase activities.The mold starter cultures were inoculated on the dry-cured pork, it was found that the inoculation of molds improved the water activity, pH, and protein degradation index of the dry-cured pork compared to the natural fermentation group.Notably, the mentioned physiochemical properties were still maintained within the optimal range for the dry-cured meat product.Furthermore, inoculating molds increased the variety of volatile flavor compounds, reduced the content of aldehydes, improved brightness and redness, and decreased shear force and thiobarbituric acid value.Among them, the P.rubens W10-1+P.fuscoglaucum V8 group performed the best in improving the quality of dry-cured pork.Electronic nose analysis showed that the flavor characteristics of the P.rubens W10-1+P.fuscoglaucum V8 group was most similar to the natural fermentation group, and also reduced lipid oxidation.Electronic tongue analysis indicated that the umami and richness of the P.rubens W10-1+P.fuscoglaucum V8 group were significantly higher than those in the natural fermentation group, while the sourness was significantly lower than that in the natural fermentation group.Therefore, the P.rubens W10-1+P.fuscoglaucum V8 group was the most suitable mold starter culture for dry-cured meat products and provided a technical support for enhancing the quality of Xuanwei hams.

参考文献

[1] WANG Z L, WANG Z X, JI L L, et al.A review:Microbial diversity and function of fermented meat products in China[J].Frontiers in Microbiology, 2021, 12:645435.
[2] LIU S Y, WANG G Y, XIAO Z C, et al.1H-NMR-based water-soluble low molecular weight compound characterization and free fatty acid composition of five kinds of Yunnan dry-cured hams[J].LWT, 2019, 108:174-182.
[3] 龙正玉, 邹金浩, 杨怀谷, 等.肉制品发酵技术对肉品品质的调控及应用研究进展[J].食品工业科技, 2024, 45(3):354-362.
LONG Z Y, ZOU J H, YANG H G, et al.Research progress on the regulation of meat quality and application of fermentation technology for meat products[J].Science and Technology of Food Industry, 2024, 45(3):354-362.
[4] GUO L P, LI Y, DING S C, et al.Effect of fermentation with two molds on characteristics of chicken meat[J].Journal of Food Quality, 2021, 2021(1):8845552.
[5] 孙萍, 王梦曼, 王淑玲, 等.娄地青霉和纳地青霉对发酵鸭肉制品理化特性和微观结构的影响[J].轻工学报, 2022, 37(4):18-25.
SUN P, WANG M M, WANG S L, et al.Effect of fermentation with enicilium roqueforti and penicilium nalgiovense on physicochemical properties and microstructure of duck meat products[J].Journal of Light Industry, 2022, 37(4):18-25.
[6] 陈杰, 孟岳成.金华火腿成熟过程中霉菌生长与其品质关系[J].食品与发酵工业, 2009, 35(8):138-142.
CHEN J, MENG Y C.Studies on the relationship between moulds and quality of Jinhua ham during ripening[J].Food and Fermentation Industries, 2009, 35(8):138-142.
[7] 李开雄, 蒋彩虹, 唐明翔, 等.霉菌在发酵香肠中的应用[J].肉类工业, 2004(12):33-36.
LI K X, JIANG C H, TANG M X, et al.Application of mold in fermented sausage[J].Meat Industry, 2004(12):33-36.
[8] DOMÍNGUEZ R, GÓMEZ M, FONSECA S, et al.Effect of different cooking methods on lipid oxidation and formation of volatile compounds in foal meat[J].Meat Science, 2014, 97(2):223-230.
[9] ZHOU C Y, WANG C, TANG C B, et al.Label-free proteomics reveals the mechanism of bitterness and adhesiveness in Jinhua ham[J].Food Chemistry, 2019, 297:125012.
[10] LYU J, YANG Z X, XU W H, et al.Relationships between bacterial community and metabolites of sour meat at different temperature during the fermentation[J].International Journal of Food Microbiology, 2019, 307:108286.
[11] WANG X W, FENG T T, WANG X J, et al.Microwave heating and conduction heating pork belly:Non-volatile compounds and their correlation with taste characteristics, heat transfer modes, and matrix microstructure[J].Meat Science, 2022, 192:108899.
[12] 万文结, 薛芷筠, 张泽文, 等.一株产碱性蛋白酶菌株的筛选鉴定及酶学特性研究[J].微生物学报, 2017, 57(5):737-747.
WAN W J, XUE Z J, ZHANG Z W, et al.Isolation and identification of an alkaline protease producing strain and study on enzymatic properties[J].Acta Microbiologica Sinica, 2017, 57(5):737-747.
[13] 徐伟良, 特格希巴雅尔, 李春冬, 等.蒙古族发霉奶豆腐中霉菌的分离鉴定与产脂肪酶活性研究[J].中国酿造, 2023, 42(7):109-113.
XU W L, TE G, LI C D, et al.Isolation and identification and lipase activity of mold in Mongolian Hurood[J].China Brewing, 2023, 42(7):109-113.
[14] 乔发东. 宣威火腿标准化生产与品质改良技术研究[D].北京:中国农业大学, 2004.
QIAO F D.Studies on the standardized production and technology of quality improving of Xuanwei ham[D].Beijing:China Agricultural University, 2004.
[15] 黎明. 复配发酵剂发酵对冕宁火腿品质的影响研究[D].成都:成都大学, 2023.
LI M.Effect of mixed starter fermentation on quality of Mianning ham[D].Chengdu:Chengdu University, 2023.
[16] OBUZ E, AKKAYA L, GÖK V, et al.Effects of blade tenderization, aging method and aging time on meat quality characteristics of Longissimus lumborum steaks from cull Holstein cows[J].Meat Science, 2014, 96(3):1227-1232.
[17] LEE C W, LEE J R, KIM M K, et al.Quality improvement of pork loin by dry aging[J].Korean Journal for Food Science of Animal Resources, 2016, 36(3):369-376.
[18] LARANJO M, ELIAS M, FRAQUEZA M J.The use of starter cultures in traditional meat products[J].Journal of Food Quality, 2017, 2017(1):9546026.
[19] 刘功明, 孙京新, 李鹏, 等.纳地青霉发酵对鸡肉质构、游离氨基酸及挥发性物质变化的影响[J].现代食品科技, 2015, 31(9):289-295.
LIU G M, SUN J X, LI P, et al.Texture, free amino acid content, and volatile compounds of chicken meat fermented by Penicillium nalgiovense[J].Modern Food Science and Technology, 2015, 31(9):289-295
[20] LÓPEZ-PEDROUSO M, PÉREZ-SANTAESCOLÁSTICA C, FRANCO D, et al.Comparative proteomic profiling of myofibrillar proteins in dry-cured ham with different proteolysis indices and adhesiveness[J].Food Chemistry, 2018, 244:238-245.
[21] ZHOU G H, ZHAO G M.Biochemical changes during processing of traditional Jinhua ham[J].Meat Science, 2007, 77(1):114-120.
[22] 周慧敏, 张顺亮, 赵冰, 等.木糖葡萄球菌和肉葡萄球菌混合发酵剂对腊肉品质的影响[J].食品科学, 2018, 39(22):32-38.
ZHOU H M, ZHANG S L, ZHAO B, et al.Effect of starter culture mixture of Staphylococcus xylosus and S.carnosus on the quality of dry-cured meat[J].Food Science, 2018, 39(22):32-38.
[23] CARRAPISO A I, GARCÍA C.Iberian ham headspace:Odorants of intermuscular fat and differences with lean[J].Journal of the Science of Food and Agriculture, 2004, 84(15):2047-2051.
[24] LI F Y, FENG X, ZHANG D N, et al.Physical properties, compositions and volatile profiles of Chinese dry-cured hams from different regions[J].Journal of Food Measurement and Characterization, 2020, 14(1):492-504.
[25] 李素, 周慧敏, 张顺亮, 等.不同加水量腌制酱牛肉中挥发性风味物质变化[J].食品科学, 2019, 40(10):199-205.
LI S, ZHOU H M, ZHANG S L, et al.Changes of volatile flavor compounds in spiced beef marinated with different water contents[J].Food Science, 2019, 40(10):199-205.
[26] ZHOU C Y, XIA Q, DU L H, et al.Recent developments in off-odor formation mechanism and the potential regulation by starter cultures in dry-cured ham[J].Critical Reviews in Food Science and Nutrition, 2023, 63(27):8781-8795.
[27] 吴鸣, 吴浚滢, 范琦琦, 等.食品风味物质的合成机理及检测方法的研究进展[J/OL].食品科学, 2024.https://link.cnki.net/urlid/11.2206.TS.20231010.0933.044.
WU M, WU J Y, FAN Q Q, et al.Research progress on synthesis mechanism and detection methods of food flavor substances[J/OL].Food Science, 2024.https://link.cnki.net/urlid/11.2206.TS.20231010.0933.044.
[28] BASSAM S M, NOLETO-DIAS C, FARAG M A.Dissecting grilled red and white meat flavor:Its characteristics, production mechanisms, influencing factors and chemical hazards[J].Food Chemistry, 2022, 371:131139.
[29] CHEN Q, HU Y Y, WEN R X, et al.Characterisation of the flavour profile of dry fermented sausages with different NaCl substitutes using HS-SPME-GC-MS combined with electronic nose and electronic tongue[J].Meat Science, 2021, 172:108338.
[30] 刘春丽. 新型腌制对发酵里脊火腿品质影响及蛋白质组学的研究[D].贵阳:贵州大学, 2020.
LIU C L.Effect of new curing on quality and proteomics of fermented loin ham[D].Guiyang:Guizhou University, 2020.
文章导航

/