食品与发酵工业 >

2024 , Vol. 50 >Issue 21: 197 - 205

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.037911

研究报告

植物基饮品中γ-氨基丁酸的富集工艺、消化吸收特性及其抗焦虑作用研究

  • 王雪 ,
  • 刘金洋 ,
  • 丁振江 ,
  • 马洪江 ,
  • 夏凯 ,
  • 刘士伟 ,
  • 曹秋阁 ,
  • 段盛林
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  • 1(黑龙江北纬四十七植物蛋白有限公司,北京,100015)
    2(中国食品发酵工业研究院有限公司,功能主食创制与慢病营养干预北京市重点实验室,北京,100015)
第一作者:博士,高级工程师(段盛林教授级高级工程师为通信作者,E-mail:dslbeijing@163.com)

收稿日期: 2023-11-09

  修回日期: 2023-11-22

  网络出版日期: 2024-11-28

基金资助

中轻集团科技创新基金项目(ZQ2022YY02)

收起

Enrichment of γ-aminobutyric acid in plant-based drinks, digestion and absorption characteristics, and its anxiolytic effects

  • WANG Xue ,
  • LIU Jinyang ,
  • DING Zhenjiang ,
  • MA Hongjiang ,
  • XIA Kai ,
  • LIU Shiwei ,
  • CAO Qiuge ,
  • DUAN Shenglin
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  • 1(Heilongjiang North Latitude 47 Plant Protein Co.Ltd., Beijing 100015, China)
    2(Beijing Key Laboratory of the Innovative Development of Functional Staple and Nutritional Intervention for Chronic Diseases, China National Research Institute of Food and Fermentation Industries Co.Ltd., Beijing 100015, China)

Received date: 2023-11-09

  Revised date: 2023-11-22

  Online published: 2024-11-28

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摘要

通过大豆胁迫发芽富集γ-氨基丁酸(γ-aminobutyric acid, GABA)是豆类产品功能化的研究热点,但富含GABA的原料与产品在消化吸收特性和抗焦虑评价方面缺乏系统研究。该研究旨在通过大豆胁迫发芽工艺富集GABA,制备富含天然GABA的植物基饮品,构建体外模拟消化和人结直肠腺癌单层细胞(Caco-2)模型,研究其消化吸收特性,构建大鼠肾上腺髓质嗜铬瘤细胞(PC12)损伤和斑马鱼焦虑模型,评估其抗焦虑作用。结果表明,热胁迫、谷氨酸钠胁迫和盐胁迫均可显著富集GABA,较对照组分别提高了243%、123%和136%;通过浸泡工艺和发芽工艺优化,确定最佳工艺为35 ℃浸泡4 h,25 ℃循环水喷淋、避光发芽24 h;体外模拟消化和Caco-2细胞吸收模型显示植物基饮品消化前后GABA含量最高,其消化产物对GABA的转运吸收能力均高于其他市售产品;此外,植物基饮品的消化产物可缓解皮质酮诱导的PC12神经细胞的损伤,促进GABA发挥PC12神经细胞保护功能,且植物基饮品具有缓解1-(3-氯苯基)哌嗪盐酸盐诱导的斑马鱼焦虑功效。

关键词： γ-氨基丁酸; 富集; 植物基饮品; 消化吸收特性; 抗焦虑

本文引用格式

王雪 , 刘金洋 , 丁振江 , 马洪江 , 夏凯 , 刘士伟 , 曹秋阁 , 段盛林 . 植物基饮品中γ-氨基丁酸的富集工艺、消化吸收特性及其抗焦虑作用研究[J]. 食品与发酵工业, 2024 , 50(21) : 197 -205 . DOI: 10.13995/j.cnki.11-1802/ts.037911

Abstract

Enrichment through soybean stress germination γ-aminobutyric acid (GABA), which enhances its anti anxiety effect, has become a research hotspot in the functionalization of soybean products, but there is a lack of systematic research.The aim of this study was to enrich GABA through soybean germination technology, prepare the plant-based drink rich in natural GABA, construct in vitro simulated digestion and Caco-2 monolayer cell models to study its digestion and absorption characteristics, construct PC12 cell damage and zebrafish anxiety model to evaluate its anti-anxiety effects.The results showed that heat stress, sodium glutamate stress, and salt stress could promote the accumulation of GABA in soybean during germination, with GABA content increased by 243%, 123%, and 136%, respectively;The optimal process for soybean germination and enrichment of GABA was to soak at 35 ℃ for 4 h, spray with circulating water at 25 ℃, and sprout in dark for 24 h;In vitro simulated digestion and Caco-2 cell absorption models showed that the plant-based drink had the highest GABA content before and after digestion, and its digestion product had higher transport and absorption capacity for GABA than other commercially available products;In addition, the digestive product of the plant-based drink could alleviate the damage of PC12 nerve cells induced by corticosterone, promote GABA to play the protective function of PC12 nerve cells, and the plant-based drink had the effect of alleviating anxiety induced by 1-(3-chlorophenyl) piperazine hydrochloride in zebrafish.

Key words： γ-aminobutyric acid; enrichment; plant-based drink; digestion and absorption; anti-anxiety

参考文献

[1] 王姗姗, 刘小娇, 胡赟, 等.植物中γ-氨基丁酸的代谢及富集机制[J].安徽农业科学, 2020, 48(24):9-12.
WANG S S, LIU X J, HU B, et al.Metabolism and enrichment mechanism of γ-aminobutyric acid in plants[J].Journal of Anhui Agricultural Sciences, 2020, 48(24):9-12.
[2] 雷玲钰, 王耀, 王莉平, 等.浅析药用植物中的γ-氨基丁酸[J].化工设计通讯, 2021, 7(2):1-2.
LEI L Y, WANG Y, WANG L P, et al.Analysis of γ-aminobutyric acid in medicinal plants[J].Chemical Engineering Design Communications, 2021, 7(2):1-2.
[3] 何梦秀, 陈芳艳, 钟杨生, 等.γ-氨基丁酸富集方法的研究进展[J].安徽农业科学, 2015, 43(15):15-17;77.
HE M X, CHEN F Y, ZHONG Y S, et al.Research progress of γ-aminobutyric acid enrichment methods[J].Journal of Anhui Agricultural Sciences, 2015, 43(15):15-17;77.
[4] 毛健, 马海乐.大豆发芽富集γ-氨基丁酸的工艺优化[J].食品科学, 2009, 30(24):227-231.
MAO J, MA H Y.Optimization of technological conditions on γ-aminobutyric accumulation in germinated soybean[J].Food Science, 2009, 30(24):227-231.
[5] YANG R Q, FENG L, WANG S F, et al.Accumulation of γ-aminobutyric acid in soybean by hypoxia germination and freeze-thawing incubation[J].Journal of the Science of Food and Agriculture, 2016, 96(6):2090-2096.
[6] 江迪, 张紫晋, 杨婷, 等.青稞麦麸γ-氨基丁酸的制备条件优化[J].中国食品学报, 2023, 23(7):259-266.
JIANG D, ZHANG Z J, YANG T, et al.Optimization of γ-aminobutyric acid preparing conditions using highland barley bran[J].Journal of Chinese Institute of Food Science and Technology, 2023, 23(7):259-266.
[7] INOUE K, SHIRAI T, OCHIAI H, et al.Blood-pressure-lowering effect of a novel fermented milk containing γ-aminobutyric acid (GABA) in mild hypertensives[J].European Journal of Clinical Nutrition, 2003, 57(3):490-495.
[8] MARQUES T M, PATTERSON E, WALL R, et al.Influence of GABA and GABA-producing Lactobacillus brevis DPC 6108 on the development of diabetes in a streptozotocin rat model[J].Beneficial Microbes, 2016, 7(3):409-420.
[9] SUWANMANON K, HSIEH P C.Effect of γ-aminobutyric acid and nattokinase-enriched fermented beans on the blood pressure of spontaneously hypertensive and normotensive Wistar-Kyoto rats[J].Journal of Food and Drug Analysis, 2014, 22(4):485-491.
[10] YAMATSU A, YAMASHITA Y, PANDHARIPANDE T, et al.Effect of oral γ-aminobutyric acid (GABA) administration on sleep and its absorption in humans[J].Food Science and Biotechnology, 2016, 25(2):547-551.
[11] BRODKORB A, EGGER L, ALMINGER M, et al.INFOGEST static in vitro simulation of gastrointestinal food digestion[J].Nature Protocols, 2019, 14(4):991-1014.
[12] MARTELLI CHAIB SALIBA A S, GIOVANINI DE OLIVEIRA SARTORI A, SOUZA BATISTA P, et al.Simulated gastrointestinal digestion/Caco-2 cell transport:Effects on biological activities and toxicity of a Brazilian propolis[J].Food Chemistry, 2023, 403:134330.
[13] 陈锐, 丁国芳, 杨最素, 等.鳕鱼皮胶原蛋白肽在Caco-2细胞单层模型中的吸收机制[J].食品科学, 2018, 39(19):154-161.
CHEN R, DING G F, YANG Z S, et al.Absorption mechanism of cod skin collagen peptide in Caco-2 cell monolayer model[J].Food Science, 2018, 39(19):154-161.
[14] ZHOU Y Z, LI X, GONG W X, et al.Protective effect of isoliquiritin against corticosterone-induced neurotoxicity in PC12 cells[J].Food & Function, 2017, 8(3):1235-1244.
[15] PRIYA M, SHARMA L, KAUR R, et al.GABA (γ-aminobutyric acid), as a thermo-protectant, to improve the reproductive function of heat-stressed mungbean plants[J].Scientific Reports, 2019, 9(1):7788.
[16] AL-QURAAN N A, SARTAWE F A B, QARYOUTI M M.Characterization of γ-aminobutyric acid metabolism and oxidative damage in wheat (Triticum aestivum L.) seedlings under salt and osmotic stress[J].Journal of Plant Physiology, 2013, 170(11):1003-1009.
[17] YANG R Q, GUO Y X, WANG S F, et al.Ca2+ and aminoguanidine on γ-aminobutyric acid accumulation in germinating soybean under hypoxia-NaCl stress[J].Journal of Food and Drug Analysis, 2015, 23(2):287-293.
[18] WANG F Z, WANG H F, WANG D H, et al.Isoflavone, γ-aminobutyric acid contents and antioxidant activities are significantly increased during germination of three Chinese soybean cultivars[J].Journal of Functional Foods, 2015, 14:596-604.
[19] 吴朝花, 何丽, 肖婷, 等.艳山姜挥发油自乳化释药系统的毒性及对Caco-2细胞单层细胞旁转运与P-糖蛋白的影响[J].中草药, 2023, 54(11):3568-3577.
WU C H, HE L, XIAO T, et al.Toxicity and effects of essential oil of Fructus Alpinia zerumbet-self-emulsifying drug delivery system on paracellular transport in Caco-2 cell monolayer and P-glycoprotein[J].Chinese Traditional and Herbal Drugs, 2023, 54(11):3568-3577.
[20] INOTSUKA R, UCHIMURA K, YAMATSU A, et al.γ-Aminobutyric acid (GABA) activates neuronal cells by inducing the secretion of exosomes from intestinal cells[J].Food & Function, 2020, 11(10):9285-9290.
[21] 马家乐, 符昭君, 王鑫玉, 等.沉香提取物对皮质酮诱导PC12细胞损伤的保护作用及机制研究[J].中草药, 2022, 53(4):1093-1099.
MA J L, FU Z J, WANG X Y, et al.Protective effect and mechanism of aquilariae lignum resinatum extract on corticosterone induced PC12 cells injury[J].Chinese Traditional and Herbal Drugs, 2022, 53(4):1093-1099.
[22] HOU C W.Pu-Erh tea and GABA attenuates oxidative stress in kainic acid-induced status epilepticus[J].Journal of Biomedical Science, 2011, 18(1):75.
[23] 班龄尹, 王笑园, 庞思成, 等.朝鲜蓟茎叶中脂溶性提取物对神经细胞的保护作用[J].中国食品学报, 2021, 21(5):31-40.
BAN L Y, WANG X Y, PANG S C, et al.Protective effect of lipophilic extracts from the stems and leaves of global artichoke on nerve cells[J].Journal of Chinese Institute of Food Science and Technology, 2021, 21(5):31-40.
[24] DING Z J, DA H H, OSAMA A, et al.Emodin ameliorates antioxidant capacity and exerts neuroprotective effect via PKM2-mediated Nrf2 transactivation[J].Food and Chemical Toxicology, 2022, 160:112790.
[25] KIRLA K T, GROH K J, POETZSCH M, et al.Importance of toxicokinetics to assess the utility of zebrafish larvae as model for psychoactive drug screening using meta-chlorophenylpiperazine (mCPP) as example[J].Frontiers in Pharmacology, 2018, 9:414.
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