食品与发酵工业 >

2024 , Vol. 50 >Issue 22: 28 - 36

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.037951

研究报告

裸藻多糖超声碱提工艺优化、结构表征及抗氧化活性研究

  • 胡锋阳 ,
  • 李市场 ,
  • 宋根娣 ,
  • 丁慧 ,
  • 常英杰 ,
  • 龚明贵
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  • 1(河南科技大学 食品与生物工程学院,河南 洛阳,471023)
    2(河南省食品微生物工程技术研究中心,河南 洛阳,471023)
    3(洛阳理工学院 环境工程与化学学院,河南 洛阳,471023)
    4(洛阳欧科拜克生物技术股份有限公司,河南 洛阳,471023)
第一作者:硕士研究生(李市场副教授为通信作者,E-mail:lscgam@sina.com)

收稿日期: 2023-11-14

  修回日期: 2024-01-16

  网络出版日期: 2024-12-17

基金资助

国家自然科学基金项目(31900005);国家自然科学基金面上项目(31870093)

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Ultrasonic-alkaline extraction, characterization, and antioxidant activity of paramylon from Euglena gracilis

  • HU Fengyang ,
  • LI Shichang ,
  • SONG Gendi ,
  • DING Hui ,
  • CHANG Yingjie ,
  • GONG Minggui
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  • 1(College of Food and Bioengineering, Henan University of Science and Technology, Luoyang 471023, China)
    2(Henan Provincial Food Microbiology Engineering Technology Research Center, Luoyang 471023, China)
    3(Department of Environmental Engineering and Chemistry, Luoyang Institute of Science and Technology, Luoyang 471023, China)
    4(Luoyang Ouke Baike Biotechnology Co.Ltd., Luoyang 471023, China)

Received date: 2023-11-14

  Revised date: 2024-01-16

  Online published: 2024-12-17

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摘要

该文探究了裸藻多糖(Euglena gracilis polysaccharide, EGP)的提取率、结构表征及体外抗氧化活性。采用超声碱提工艺结合Plackett-Burman和响应面设计对EGP提取率进行优化,采用凝胶渗透色谱、离子色谱、傅里叶变换红外光谱、扫描电镜和自由基清除实验对EGP的分子质量、单糖组成、基团构成、微观结构和抗氧化活性进行分析。超声碱提法用于EGP提取的最佳工艺参数为料液比为1∶40(g∶mL),水浴温度为41 ℃,水浴时间为0.9 h,NaOH溶液浓度为1 mol/L,超声功率为120 W,超声时间为3 min,在此条件下,EGP的提取率为(62.84±0.49)%。结果表明,EGP由100%葡萄糖组成,通过β-糖苷键连接,分子质量为2.13×104Da。抗氧化实验结果表明,EGP对羟自由基、DPPH自由基、ABTS阳离子自由基、超氧阴离子自由基均表现出良好的抗氧化活性。EGP的结构研究为裸藻的继续开发利用提供了科学的理论依据。

关键词： 裸藻; 裸藻多糖; 响应面法; 结构表征; 抗氧化活性

本文引用格式

胡锋阳 , 李市场 , 宋根娣 , 丁慧 , 常英杰 , 龚明贵 . 裸藻多糖超声碱提工艺优化、结构表征及抗氧化活性研究[J]. 食品与发酵工业, 2024 , 50(22) : 28 -36 . DOI: 10.13995/j.cnki.11-1802/ts.037951

Abstract

Euglena gracilis polysaccharide (EGP), as a new natural functional food, has attracted more and more attention.This study investigated the extraction yield, structural characterization, and in vitro antioxidant activity of EGP.The extraction yield EGP was optimized with ultrasonic-alkali extraction by Plackett-Burman and response surface design.The molecular weight, monosaccharide composition, group composition, microstructure, and antioxidant activity of EGP were analyzed using gel permeation chromatography (GPC), ion chromatography (IC), Fourier transform infrared spectroscopy (FT-IR), scanning electron microscope (SEM), and free radical scavenging tests.The optimum process conditions were as follows:the liquid-to-material ratio of 1∶40 (g∶mL), water bath temperature of 41 ℃, water bath time of 0.9 h, NaOH solution concentration of 1 mol/L, ultrasonic power of 120 W, and ultrasonic time of 3 min.Under these conditions, the extraction yield was (62.84±0.49)%.Results indicated that EGP was composed of 100% glucose and connected by β-glycosidic bonds, and the molecular weight were 2.13×104 Da.Antioxidant experiments demonstrated that EGP showed good antioxidant activity against ·OH, DPPH free radicals, ABTS cationic radicals, and ·O-2.The structural study of EGP provides a scientific theoretical basis for the continued development and utilization of Euglena gracilis.

Key words： Euglena gracilis; Euglena gracilis polysaccharide; response surface method; structural characterization; antioxidant activity

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