熊果苷是一种具有抗炎、抑菌、镇咳、美白等多种药理活性的糖苷类化合物,但其脂溶性差、生物利用度低,限制了其应用范围。全细胞催化其酰化反应,可获得具有生物活性好及生物利用度高的熊果苷酯衍生物。由于目前全细胞原始培养基配方所得的细胞生物量较低,故该研究以熊果苷辛酰化为反应模型,以米曲霉为菌种,探究了全细胞培养条件对细胞生物量及其催化效率的影响,以期获得较多生物量、较高催化活性的全细胞催化剂。结果表明,米曲霉在6.0 g/L大豆油、7.0 g/L胰蛋白胨、5.0 g/L (NH4)2SO4和0.2 g/L CaCl2的培养基中,于30 ℃、180 r/min培养48 h,米曲霉全细胞生物量达到6.28 g/L,较初始培养基提高了4.39倍,熊果苷转化率达99.55%。该研究设计的可催化熊果苷酰化反应的新型微生物全细胞培养体系,具有成本低、高效、高选择性等优点,为熊果苷酯衍生物的工业化制备提供了有效途径。
Arbutin is a glycoside compound with various pharmacological activities such as anti-inflammatory, antibacterial, antitussive, and whitening, but its poor lipid solubility and low bioavailability limit its application range.The arbutin derivatives obtained by whole-cell catalysis have better bioactivity and bioavailability.However, the cell biomass from the original medium formula was low.In this paper, the effects of whole-cell culture conditions on cell biomass and catalytic efficiency were investigated by using arbutin caproylation as the reaction model and Aspergillus oryzae as the strain to obtain whole-cell catalysts with more biomass and higher catalytic activity.The biomass reached 6.28 g/L in the medium containing 6.0 g/L soybean oil, 7.0 g/L tryptone, 5.0 g/L (NH4)2SO4, and 0.2 g/L CaCl2 at 180 r/min and 30 ℃ for 48 h, which was 4.39-folds higher than that in the initial conditions, and the arbutin conversion reached 99.55%.The new microbial whole-cell culture system that can catalyze arbutin acylation reaction has advantages including low cost, high efficiency, and high selectivity.This study provides an effective strategy for the industrial preparation of arbutin ester derivatives.
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