牦牛乳作为青藏高原特色乳资源,功能性产品研究与转化较少,不利于地方经济的发展。为探究甘南牦牛乳的抗氧化活性,该实验选取羟自由基、超氧阴离子自由基、DPPH自由基开展牦牛乳体外抗氧化效果评价;优选受精后3 dpf的斑马鱼,在不同添加量(12.5、25.0、50.0 μL/mL)的牦牛乳中暴露到24 h,通过荧光染色检测斑马鱼活性氧(reactive oxygen species, ROS)产生率,分析超氧化物歧化酶活力,进行牦牛乳体内抗氧化效果评价。结果表明,牦牛乳具有清除羟自由基、DPPH自由基以及超氧阴离子自由基的能力;12.5、25.0、50.0 μL/mL添加量的牦牛乳能降低斑马鱼体内ROS产生率,提高斑马鱼抗氧化酶活力,说明牦牛乳具有抗氧化活性,且牦牛乳体内抗氧化效果与其质量浓度呈正相关,当牦牛乳添加量为50.0 μL/mL,对斑马鱼的抗氧化效果达到43.25%。实验表明,甘南牦牛乳具有抗氧化活性,可作为抗氧化功能性食品的原料开发。
As a characteristic milk resource of the Qinghai-Tibet Plateau, yak milk was less researched and transformed into functional products, which was not conducive to the development of the local economy.To investigate the antioxidant activity of Gannan yak milk, hydroxyl radicals, superoxide anions, and DPPH radicals were selected to evaluate the antioxidant effect of yak milk in vitro, and zebrafish with 3 dpf after fertilization were exposed to yak milk with different additions (12.5, 25.0, 50.0 μL/mL) for 24 h.The production rate of reactive oxygen species (ROS) and the activity of superoxide dismutase in zebrafish were detected by fluorescent staining, and the antioxidant effect in yak milk was evaluated.Results showed that yak milk had the ability to scavenge hydroxyl free radicals, DPPH free radicals, and superoxide anions.12.5 μL/mL, 25.0 μL/mL, and 50.0 μL/mL addition of yak milk could reduce ROS production rate and increase antioxidant oxidase activity in zebrafish, which indicated that yak milk has antioxidant activity, and the antioxidant effect in yak milk was positively correlated with its mass concentration.When the addition of yak milk was 50.0 μL/mL, the antioxidant effect on zebrafish reached 43.25%.Results showed that Gannan yak milk had antioxidant activity and could be used as a raw material for antioxidant functional food.
[1] LIN K, ZHANG L W, HAN X, et al.Yak milk casein as potential precursor of angiotensin I-converting enzyme inhibitory peptides based on in silico proteolysis[J].Food Chemistry, 2018, 254:340-347.
[2] 宋礼, 罗丽, 李顺成, 等.甘南牦牛乳功能性营养成分含量研究[J].食品工业, 2022, 43(10):323-325.
SONG L, LUO L, LI S C, et al.Study on the contents of functional nutrients in Gannan yak milk[J].The Food Industry, 2022, 43(10):323-325.
[3] DRAGANIDIS D, KARAGOUNIS L G, ATHANAILIDIS I, et al.Inflammaging and skeletal muscle:Can protein intake make a difference[J].The Journal of Nutrition, 2016, 146(10):1940-1952.
[4] 崔光欣. 青藏高原高寒植物及牦牛奶抗氧化特性研究[D].兰州:兰州大学, 2016.
CUI G X.Antioxidant property of alpine plants and yak milk on Qinghai-Tibetan Plateau[D].Lanzhou:Lanzhou University, 2016.
[5] ZHAO L L, DU M, GAO J, et al.Label-free quantitative proteomic analysis of milk fat globule membrane proteins of yak and cow and identification of proteins associated with glucose and lipid metabolism[J].Food Chemistry, 2019, 275:59-68.
[6] 郭咏梅. 硒通过TrxRl、MAPK信号通路缓解奶牛乳腺上皮细胞氧化应激的机理研究[D].呼和浩特:内蒙古农业大学, 2020.
GUO Y M.Mechanism of Selenium alleviating oxidative stress in bovine mammary epithelial cells through modulation of TrxRl/ MAPK signaling pathways[D].Hohhot:Inner Mongolia Agricultural University, 2020.
[7] 高宇, 汪家琦, 戴智勇, 等.牦牛乳营养组分及功能特性研究进展[J].乳业科学与技术, 2021, 44(3):43-49.
GAO Y, WANG J Q, DAI Z Y, et al.Review of research on nutritional components and functional characteristics of yak milk[J].Journal of Dairy Science and Technology, 2021, 44(3):43-49.
[8] ZHANG J Z, LI D F.Effect of conjugated linoleic acid on inhibition of prolyl hydroxylase 1 in hearts of mice[J].Lipids in Health and Disease, 2012, 11:22.
[9] 代安娜, 杨具田, 丁波, 等.牦牛乳组分及功能特性研究进展[J].动物营养学报, 2022, 34(6):3443-3453.
DAI A N, YANG J T, DING B, et al.Research progress on components and functional characteristics of yak milk[J].Chinese Journal of Animal Nutrition, 2022, 34(6):3443-3453.
[10] WANG D D, ZHOU Y X, ZHENG X P, et al.Yak milk:Nutritional value, functional activity, and current applications[J].Foods, 2023, 12(11):2090.
[11] MOON J, LEE S, JEONG S, et al.Free radical-scavenging activities and cytoprotective effect of polyphenol-rich ethyl acetate fraction of guava (Psidium cattleianum) leaves on H2O2-treated HepG2 cell[J].Journal of the Korean Society for Applied Biological Chemistry, 2013, 56:687-694.
[12] YANG Y, ZHENG K D, MEI W J, et al.Anti-inflammatory and proresolution activities of bergapten isolated from the roots of Ficus hirta in an in vivo zebrafish model[J].Biochemical & Biophysical Research Communications, 2018, 496(2):763-769.
[13] KIM E A, LEE S H, KO C I, et al.Protective effect of fucoidan against AAPH-induced oxidative stress in zebrafish model[J].Carbohydrate Polymers, 2014, 102:185-191.
[14] 李爽, 刘小芳, 冷凯良, 等.大西洋鳕鱼骨胶原蛋白肽的抗氧化活性及稳定性研究[J].食品与发酵工业, 2024,50(16):50-56.
LI S, LIU X F, LENG K L, et al.Antioxidant activity and stability of collagen peptides from Atlantic cod (Gadus morhua) bone[J].Food and Fermentation Industries, 2024,50(16):50-56.
[15] 卢野. 混合驴乳发酵配方优化及体外抗氧化研究[D].阿拉尔:塔里木大学, 2021.
LU Y.Optimization of fermentation formula and in vitro antioxidation of mixed donkey milk[D].Ala’er:Tarim University, 2021.
[16] 刘海波, 支媛, 汪会玲, 等.基于斑马鱼幼鱼的抗氧化物筛选模型建立及其应用[J].中国食品卫生杂志, 2022, 34(5):871-877.
LIU H B, ZHI Y, WANG H L, et al.Establishment and application of antioxidant screening model based on zebrafish larva[J].Chinese Journal of Food Hygiene, 2022, 34(5):871-877.
[17] HE Z, GUO Y J, CHEN J Z, et al.Unsaturated phospholipid modified FeOCl nanosheets for enhancing tumor ferroptosis[J].Journal of Materials Chemistry. B, 2023, 11(9):1891-1903.
[18] 郑鑫鑫, 黄青.天然虾青素清除超氧阴离子的抗氧化能力[J].食品研究与开发, 2023, 44(14):8-12;162.
ZHENG X X, HUANG Q.Antioxidant activity of natural astaxanthin for scavenging superoxide anion[J].Food Research and Development, 2023, 44(14):8-12;162.
[19] 王银玲, 薛玉洋, 孙浩彬, 等.桑叶提取物体外抗氧化活性及其对黄羽肉鸡抗氧化能力的影响[J].动物营养学报, 2023, 35(8):5059-5068.
WANG Y L, XUE Y Y, SUN H B, et al.In vitro antioxidant activity of mulberry leaf extract and its effects on antioxidant capacity of yellow feather broilers[J].Chinese Journal of Animal Nutrition, 2023, 35(8):5059-5068.
[20] BARNHAM K J, MASTERS C L, BUSH A I.Neurodegenerative diseases and oxidative stress[J].Nature Reviews. Drug Discovery, 2004,3(3):205-214.
[21] 徐懿乔, 陈星辉, 黄燕烽, 等.一种利用斑马鱼模型评价抗氧化和淡斑亮肤功效的方法:中国, CN112107697A[P].2020-12-22.
XU Y Q, CHEN X H, HUANG Y F, et al.A method for evaluating antioxidant and lightening-spot brightening-skin efficacy using zebrafish model:China, CN112107697A[P].2020-12-22.
[22] BLADEN C L, KOZLOWSKI D J, DYNAN W S.Effects of low-dose ionizing radiation and menadione, an inducer of oxidative stress, alone and in combination in a vertebrate embryo model[J].Radiation Research, 2012, 178(5):499-503.
[23] ZHANG W, CAO J L, WU S Z, et al.Anti-fatigue effect of yak milk powder in mouse model[J].Dairy Science & Technology, 2015, 95(2):245-255.
[24] 朱文卿, 朱姗姗, 何秋霞, 等.牛蒡多糖与绿原酸对斑马鱼氧化损伤的协同抗氧化作用[J].中国食品学报, 2022, 22(4):95-103.
ZHU W Q, ZHU S S, HE Q X, et al.Synergistic antioxidant effect of burdock polysaccharide and chlorogenic acid on oxidative damage in zebrafish[J].Journal of Chinese Institute of Food Science and Technology, 2022, 22(4):95-103.
