食品与发酵工业 >

2024 , Vol. 50 >Issue 24: 182 - 190

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.038639

研究报告

新鞘氨醇杆菌胞外多糖的发酵优化及结构和性质表征

  • 姬淑洁 ,
  • 杨传佳 ,
  • 陈修来 ,
  • 陆健 ,
  • 李晓敏
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  • 1(江南大学 生物工程学院,江苏 无锡,214122)
    2(粮食发酵与食品生物制造国家工程研究中心(江南大学),江苏 无锡,214122)
第一作者:硕士研究生(李晓敏副教授为通信作者,E-mail:xiaomin@jiangnan.edu.cn)

收稿日期: 2024-01-19

  修回日期: 2024-02-27

  网络出版日期: 2024-12-30

基金资助

江苏省农业科技自主创新资金项目 (CX(23)2005)

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Fermentation optimization, structural and property characterization of exopolysaccharides from Nosphingobium sp.

  • JI Shujie ,
  • YANG Chuanjia ,
  • CHEN Xiulai ,
  • LU Jian ,
  • LI Xiaomin
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  • 1(School of Biotechnology, Jiangnan University, Wuxi 214122, China)
    2(National Engineering Research Center of Cereal Fermentation and Food Biomanufacturing, Jiangnan University, Wuxi 214122, China)

Received date: 2024-01-19

  Revised date: 2024-02-27

  Online published: 2024-12-30

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摘要

从福建药白曲中分离得到一株产胞外多糖的新鞘氨醇杆菌(Nosphingobium sp.)D6菌株,通过单因素试验和响应面法对其产胞外多糖发酵条件进行优化,在最佳发酵条件下,即蔗糖添加量为39.0 g/L、豆粕粉添加量为3.6 g/L、硫酸锰添加量为0.4 g/L、发酵温度30 ℃,转速250 r/min,装液量100 mL/500 mL,种龄24 h,接种比例5%(体积分数),胞外多糖产量为(22.38±0.43) g/L。相较于优化前,胞外多糖产量提高了5.87倍。该胞外多糖由甘露糖、半乳糖醛酸、葡萄糖和半乳糖4种单糖组成,且具有乙酰基修饰,该多糖水溶液具有假塑性流体特征,同时表现出较好的乳化性和热稳定性。

关键词： 新鞘氨醇杆菌; 胞外多糖; 发酵优化; 单糖组成; 乳化性

本文引用格式

姬淑洁 , 杨传佳 , 陈修来 , 陆健 , 李晓敏 . 新鞘氨醇杆菌胞外多糖的发酵优化及结构和性质表征[J]. 食品与发酵工业, 2024 , 50(24) : 182 -190 . DOI: 10.13995/j.cnki.11-1802/ts.038639

Abstract

A Nosphingobium sp.strain D6 that could produce extracellular polysaccharide (EPS) was isolated from Fujian white koji.The fermentation conditions were optimized through single-factor tests and the response surface method.Under the optimal fermentation conditions, which were sucrose at 39.0 g/L, soybean meal at 3.6 g/L, manganese sulfate at 0.4 g/L, fermentation temperature at 30 ℃, rotation speed at 250 r/min, liquid loading volume at 100 mL/500 mL, seed age at 24 h, inoculation ratio at 5% (v/v), the EPS yield reached (22.38±0.43) g/L, making an increase of 5.87 times.The EPS of D6 comprised of four types of monosaccharide, including mannose, galacturonic acid, glucose and galactose, and was modified with acetyl groups.Additionally, the EPS aqueous solution demonstrated pseudoplastic fluid characteristics, and exhibited good emulsifying properties and thermal stability.

Key words： Novosphingobium; exopolysaccharide; fermentation optimize; monosaccharide composition; emulsification

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