蛋白质是一种相对稳定存在于蜂蜜中的大分子物质,研究蜂蜜蛋白对探究蜂蜜生物活性,筛选溯源标志成分具有重要意义。该文针对中国特色单花种蜂蜜——枣花蜜,基于凝胶和非凝胶2种蛋白质组学技术鉴定分析采集枣花蜜样品中的蛋白质组成,共发现216个蜂源蛋白和595个植物源蛋白,其中24种蜂源蛋白首次在枣花蜜中鉴定到,包括MAGUK p55 亚族成员6 X1亚型、几丁质结合2型结构域蛋白、肽酶S1结构域蛋白等;利用BLAST工具筛选了11个潜在的枣花蜜特征蛋白标志物,包括金属内切蛋白酶2-MMP样、FAS1结构域蛋白、解毒蛋白质等。GO功能注释分析发现枣花蜜中鉴定到的蜂源和植物源蛋白主要参与碳水化合物代谢、蛋白质水解等生物学过程,涉及ATP结合、三磷酸鸟苷结合等分子功能。该文基于2种蛋白组学分析方法探究了枣花蜜蛋白质组成,为研究枣花蜜蛋白生物活性成分及筛选特征蛋白标志物提供理论参考。
Protein is a relatively stable macromolecular substance existing in honey, and the research on honey protein is of great significance in exploring honey biological activity and screen marker ingredients.Jujube (Ziziphus jujuba Mill.) honey, a characteristic monofloral honey in China, was selected to investigate its protein components by gel-based and gel-free proteomics.Results showed that 216 bee-derived and 595 plant-derived proteins were identified in the collected samples.Among these proteins, 24 bee-derived proteins were first reported in jujube honey, including MAGUK p55 subfamily member 6 isoform X1, chitin-binding type-2 domain-containing protein, peptidase S1 domain-containing protein, and so on.Notably, by BLAST analysis, 11 proteins, including metalloendoproteinase 2-MMP-like, FAS1 domain-containing protein, peptidase S1 domain-containing protein, etc.could be considered as potential markers for jujube honey.GO analysis revealed these bee-derived and plant-derived proteins mainly were involved in the processes of carbohydrate metabolic and proteolysis, and were associated with the functions of ATP binding and guanosine triphosphate binding.The research comprehensively investigated the jujube protein components, and provided a theoretical reference for investigating the protein bioactive components and screening unique protein makers in jujube honey.
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