食品与发酵工业 >

2025 , Vol. 51 >Issue 2: 176 - 184

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.038596

研究报告

连翘生物保鲜剂对冬枣采后链格孢菌的抑菌机理

  • 冯云芳 ,
  • 冯志宏 ,
  • 张新宪 ,
  • 高振峰 ,
  • 贾艳兰
展开
  • (山西农业大学 食品科学与工程学院,山西 太原,030000)
第一作者:硕士研究生(冯志宏副研究员为通信作者,E-mail:fengzh100@163.com)

收稿日期: 2024-01-17

  修回日期: 2024-03-11

  网络出版日期: 2025-02-14

基金资助

山西省重点研发计划项目(201903D211007-2);山西省重点研发计划(农业)项目(2015-TN-4-6)

收起

Inhibitory mechanism of Forsythia suspensa biological preservative on Alternaria alternata after harvest of winter jujube

  • FENG Yunfang ,
  • FENG Zhihong ,
  • ZHANG Xinxian ,
  • GAO Zhenfeng ,
  • JIA Yanlan
Expand
  • (College of Food Science and Engineering, Shanxi Agricultural University, Taiyuan 030000, China)

Received date: 2024-01-17

  Revised date: 2024-03-11

  Online published: 2025-02-14

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摘要

为了探究连翘生物保鲜剂对冬枣采后链格孢菌的抗菌活性及其机制,通过体外抑菌实验测定最小抑菌浓度(minimum inhibitory concentration,MIC)和最小杀菌浓度(minimum bactericidal concentration,MBC),同时结合连翘生物保鲜剂对链格孢菌细胞成分的释放、细胞膜通透性、胞内酶活力及菌丝形态等的影响,进一步阐明连翘生物保鲜剂对链格孢菌的抑菌机理。结果表明,连翘生物保鲜剂对链格孢菌的MIC是25 mg/L,MBC是100 mg/L;经100 mg/L连翘生物保鲜剂处理后,增强了链格孢菌细胞膜通透性,导致核酸、可溶性蛋白、丙二醛(malondialdehyde,MDA)的含量和相对电导率显著上升;不仅抑制了链格孢菌Na+/K+-ATPase及谷胱甘肽还原酶(glutathione reductase,GR)活力,使其活力分别降低了0.58倍、1.8倍;而且显著降低了麦角甾醇的含量和呼吸抑制率。综上所述,连翘生物保鲜剂对链格孢菌具有良好的抑菌效果,通过破坏链格孢菌细胞膜结构、改变膜通透性、干扰能量代谢等途径发挥抑菌作用。

关键词： 连翘生物保鲜剂; 冬枣; 链格孢菌; 抑菌

本文引用格式

冯云芳 , 冯志宏 , 张新宪 , 高振峰 , 贾艳兰 . 连翘生物保鲜剂对冬枣采后链格孢菌的抑菌机理[J]. 食品与发酵工业, 2025 , 51(2) : 176 -184 . DOI: 10.13995/j.cnki.11-1802/ts.038596

Abstract

To explore the antibacterial activity and mechanism of Forsythia suspensa biological preservative against Alternaria ziziphi, the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined by in vitro antibacterial test.At the same time, combined with the effects of Forsythia biological preservative on the release of cell components, cell membrane permeability, intracellular enzyme activity, and mycelium morphology of Alternaria, the bacteriostatic mechanism of Forsythia biological preservative on Alternaria was further clarified.Results showed that the MIC and MBC of Forsythia suspensa biological preservative against Alternaria alternata were 25 mg/L and 100 mg/L respectively.After being treated with 100 mg/L Forsythia biological preservative, the cell membrane permeability of Alternaria alternata was enhanced, resulting in a significant increase in the contents of nucleic acid, soluble protein and malondialdehyde (MDA), and the relative conductivity.It not only inhibited the activities of Na+/K+-ATPase and glutathione reductase (GR) of Alternaria alternata, reduced their activities by 0.58 times and 1.8 times respectively, but also significantly reduced ergosterol content and respiratory inhibition rate.To sum up, Forsythia suspensa biological preservative has a good antibacterial effect on Alternaria alternata, which can be achieved by destroying the cell membrane structure of Alternaria alternata, changing the membrane permeability and interfering with energy metabolism.

Key words： Forsythia suspense biological preservative; winter jujube; Alternaria alternata; bacteriostat

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