食品与发酵工业 >

2025 , Vol. 51 >Issue 3: 64 - 71

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.038698

研究报告

戊糖乳杆菌Z097提取物对单增李斯特菌群体感应信号分子和生物被膜的抑制作用

  • 叶国良 ,
  • 张杏果 ,
  • 黄铭新 ,
  • 李婷 ,
  • 钟青萍
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  • (华南农业大学 食品学院, 广东省食品质量安全重点实验室, 广东 广州, 510642)
第一作者:硕士研究生(钟青萍教授为通信作者,E-mail:zhongqp@scau.edu.cn)

收稿日期: 2024-01-24

  修回日期: 2024-03-14

  网络出版日期: 2025-02-21

基金资助

广东省基础与应用基础研究基金项目(2021A1515011083);国家自然科学基金面上项目(31972046);广东省科技计划项目(2020B1212060059)

收起

Inhibition effects of Lactiplantibacillus pentosus Z097 extract against Listeria monocytogenes quorum sensing signaling molecule and biofilm

  • YE Guoliang ,
  • ZHANG Xingguo ,
  • HUANG Mingxin ,
  • LI Ting ,
  • ZHONG Qingping
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  • (Guangdong Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou 510642, China)

Received date: 2024-01-24

  Revised date: 2024-03-14

  Online published: 2025-02-21

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摘要

单增李斯特菌是常见的食源性致病菌,采用乳酸菌的活性物质淬灭其群体感应(quorum sensing,QS)系统和抑制生物被膜形成以控制单增李斯特菌具有重要的研究价值。该研究测定了戊糖乳杆菌Z097的乙酸乙酯提取物(Z097-E)对单增李斯特菌的最小抑菌浓度(minimum inhibitory concentration,MIC),探究Z097-E在亚抑菌浓度下对其生长、自聚性、疏水性、运动性、生物被膜形成、胞外多糖、胞外蛋白和QS信号分子AI-2等的影响,并测定了其不同浓度对成熟生物被膜的清除效果。结果表明,Z097-E的MIC为6.4 mg/mL;亚抑菌浓度3.2 mg/mL的Z097-E处理使单增李斯特菌的自聚性和疏水性分别下降了73.58%和89.62%;同时对单增李斯特菌的胞外多糖、胞外蛋白、AI-2活性的抑制率分别为80.03%、34.23%和84.43%;此浓度处理24 h和48 h后,生物被膜量分别降低了52.76%和53.77%。以12.8 mg/mL的Z097-E处理成熟生物被膜24、48 h后,对其清除率分别为30.08%和62.07%。结果表明,Z097-E对单增李斯特菌的生物被膜和群体感应具有显著的抑制作用,为在食品加工、运输和保藏过程中单增李斯特菌生物被膜的控制提供了参考依据。

关键词： 戊糖乳杆菌; 单增李斯特菌; 群体感应; 生物被膜; 抑制作用

本文引用格式

叶国良 , 张杏果 , 黄铭新 , 李婷 , 钟青萍 . 戊糖乳杆菌Z097提取物对单增李斯特菌群体感应信号分子和生物被膜的抑制作用[J]. 食品与发酵工业, 2025 , 51(3) : 64 -71 . DOI: 10.13995/j.cnki.11-1802/ts.038698

Abstract

Listeria monocytogenes is a common foodborne pathogen.Active substances of lactic acid bacteria have potential abilities to control L.monocytogenes through quenching quorum sensing (QS) systems and inhibiting biofilm formation.In this study, the minimum inhibitory concentration (MIC) of ethyl acetate extract of Lactiplantibacillus pentosus Z097 (Z097-E) against L.monocytogenes was determined, and the effects of sub-MIC of Z097-E on the growth, auto-aggregation, hydrophobicity, motility, biofilm formation, exopolysaccharide, extracellular protein, and QS signal AI-2 of L.monocytogenes were explored.The elimination effects of different concentrations of Z097-E on mature biofilm were also measured.Results showed that the MIC of Z097-E was 6.4 mg/mL.At 3.2 mg/mL of Z097-E, the auto-aggregation rate and hydrophobicity of L.monocytogenes were decreased by 73.58% and 89.62%, respectively.Meanwhile, the inhibition rates on exopolysaccharide, extracellular proteins, and AI-2 of L.monocytogenes were 80.03%, 34.23%, and 84.43%, respectively.When treated with 3.2 mg/mL of Z097-E for 24 and 48 hours, the biofilm biomass was decreased by 52.76% and 53.77%, respectively.The elimination rates of 12.8 mg/mL of Z097-E on mature biofilm for 24 h and 48 h were 30.08% and 62.07%, respectively.Results showed that the Z097-E could inhibit the biofilm and quorum sensing of L.monocytogenes, providing a reference for the control of L.monocytogenes biofilm in food processing, transportation, and preservation processes.

Key words： Lactiplantibacillus pentosus; Listeria monocytogenes; quorum sensing; biofilm; inhibitory effects

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