随着人口的快速增长,全球范围内对优质蛋白质的需求日益增强,通过食用菌育种技术培育高产、高蛋白的优质菌株,将菌丝体蛋白用作新的蛋白质来源,可以缓解蛋白质短缺的问题。该研究以桃红侧耳GXGD-13-1为原始菌株,通过原生质体细胞融合技术,提高桃红侧耳菌丝体的蛋白质产量。菌丝体的细胞壁在组合酶M2中酶解3 h,释放的原生质体利用250 g/L的聚乙二醇为融合剂进行聚集融合,最终融合率达到53%,有效克服了桃红侧耳四极性的交配系统亲和率低的育种壁垒。融合再生后的菌株经过选育高产菌丝蛋白菌株的初筛和复筛,得到融合菌株R-1,经凯氏定氮仪测定,计算其菌丝体蛋白质产量比原始菌株GXGD-13-1提高约23%。该文的实验工作,证明了原生质体细胞融合育种技术对选育高产菌丝体蛋白质菌株的有效性,为满足不断增长的蛋白质需求提供新的有效途径。
With the rapid growth of population, the global demand for high-quality protein is increasing day by day.To alleviate the problem of protein shortage, mycelium can be used as a new source of protein through the cultivation of high-quality strains of edible fungi breeding.In this study, Pleurotus djamor GXGD-13-1 was used as the original strain, protoplasmic cell fusion technique was used to increase its protein yield.First mycelium cell walls were enzymolysised in M2 composite for 3 hours, then released protoplast was fused with polyethylene glycol (PEG) in which 250 g/L of PEG was used as the fusion agent.A high fusion rate of 53% was achieved after the process, removing the breeding barrier of low compatibility of the tetrpolarity mating system.Primary screening and re-screening of fusion strain R-1 were carried out to select a high mycelial protein yield, 23% higher than that of the original strain GXGD-13-1.The work in this paper proved the efficiency of protoplasmic somatic cell fusion breeding technology in the selection of high-yield mycelium protein strains and provided an alternative way to meet the increasing demand for protein.
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