食品与发酵工业 >

2025 , Vol. 51 >Issue 4: 255 - 263

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.038985

研究报告

微拟球藻分级醇沉多糖的理化特性与抗氧化活性

  • 林丽芹 ,
  • 王宝贝 ,
  • 肖金艳 ,
  • 向瑞琦 ,
  • 路亚辉 ,
  • 加晶
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  • 1(泉州师范学院 化工与材料学院,福建 泉州,362000)
    2(泉州师范学院 海洋与食品学院福建省海洋藻类活性物质制备与功能开发重点实验室,福建 泉州,362000)
    3(近海资源生物技术福建省高校重点实验室,福建 泉州,362000)
    4(福州大学 先进制造学院,福建 泉州,362251)
    5(微藻生物能源与资源北京市重点实验室,北京,100142)
    6(国家开发投资集团有限公司国投生物科技投资有限公司国投微藻生物科技中心,北京,100034)
第一作者:博士,讲师(王宝贝教授为通信作者,E-mail:baobeiw@qztc.edu.cn)

收稿日期: 2024-02-27

  修回日期: 2024-04-12

  网络出版日期: 2025-03-10

基金资助

泉州市科技计划项目(2020C027R);福建省科技厅自然科学基金(2020J01786);福建省重点实验室建设项目(2022KF08)

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Physicochemical characterization and antioxidant activities of polysaccharides from Nannochloropsis by gradient ethanol precipitation

  • LIN Liqin ,
  • WANG Baobei ,
  • XIAO Jinyan ,
  • XIANG Ruiqi ,
  • LU Yahui ,
  • JIA Jing
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  • 1(College of Chemical Engineering and Materials Science, Quanzhou Normal University, Quanzhou 362000, China)
    2(Fujian Province Key Laboratory for the Development of Bioactive Material from Marine Algae, College of Oceanology and Food Science, Quanzhou Normal University, Quanzhou 362000, China)
    3(Key Laboratory of Inshore Resources and Biotechnology Fujian Province University, Quanzhou 362000, China)
    4(School of Advanced Manufacturing, Fuzhou University, Quanzhou 362251, China)
    5(Beijing Key Laboratory of Microalgae Bioenergy and Bioresource, Beijing 100142, China)
    6(SDIC Microalgae Biotechnology Center, SDIC Biotechnology Investment Co.Ltd., State Development and Investment Corporation, Beijing 100034, China)

Received date: 2024-02-27

  Revised date: 2024-04-12

  Online published: 2025-03-10

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摘要

为促进微拟球藻多糖的高值化利用,该研究采用40%、60%和80%(体积分数)的乙醇分级沉淀微拟球藻多糖得到NSP40、NSP60和NSP80 3个组分,通过高效液相色谱、凝胶色谱-示差-多角度激光光散射系统(high pressure gel chromatography-muitiple angle light scattering detector-differential refractive index detector, HPSEC-MALS-RI)、傅里叶变换红外光谱(Fourier transform infrared spectrometer, FT-IR)和流变仪等方法对多糖的单糖组成、分子质量、结构、流变特性等理化性质进行分析,并评价其抗氧化活性。结果表明,多糖得率、总糖含量与乙醇体积分数呈正相关。当乙醇体积分数为80%时,多糖得率和总糖含量最高,分别为(3.48±0.04)%和(74.43±0.76)%。NSP的糖醛酸含量、单糖组成、分子质量分布等理化性质受乙醇体积分数影响。其中,NSP60的平均分子质量和黏度是所有NSP中最高的。3个NSP组分均为Glc、Man、Gal、Rib、Rha、Fuc、Xyl、Ara和Glc-UA 9种单糖为单元构建的杂多糖,各组分间的单糖摩尔比差异较大。抗氧化分析结果表明,NSP40的ABTS阳离子自由基、羟自由基的清除能力最强,而NSP60的总还原力最强。研究结果表明,分级醇沉法可以用于微拟球藻多糖的初步分离,获得不同理化性质和生物活性的多糖组分。

关键词： 微拟球藻; 多糖; 分级醇沉; 理化特性; 抗氧化活性

本文引用格式

林丽芹 , 王宝贝 , 肖金艳 , 向瑞琦 , 路亚辉 , 加晶 . 微拟球藻分级醇沉多糖的理化特性与抗氧化活性[J]. 食品与发酵工业, 2025 , 51(4) : 255 -263 . DOI: 10.13995/j.cnki.11-1802/ts.038985

Abstract

To promote the high-value utilization of Nannochloropsis polysaccharides, gradient ethanol precipitation was applied to separate Nannochloropsis soluble polysaccharide (NSP).Three fractions (NSP40, NSP60, and NSP80) were obtained by 40%, 60%, and 80% ethanol precipitation sequentially.The physicochemical characteristics including monosaccharide composition, molecular weight, structure, and rheological properties of polysaccharides were analyzed by HPLC, high pressure gel chromatography-muitiple angle light scattering detector-differential refractive index detector, Fourier transform infrared spectrometer, and rheometer.The antioxidant activities of each fraction were also evaluated.Results showed that the yield and total sugar content of polysaccharide precipitated were positively correlated with ethanol concentration.When the ethanol concentration was 80%, the yield and total sugar content was (3.48±0.04) % and (74.43±0.76)%, respectively.The content of uronic acid, monosaccharide composition, molecular weight distribution, and viscosity of NSP were affected by ethanol concentration.In which, NSP60 possessed the highest average molecular weight and viscosity.All of the three NSP fractions isolated were heteropolysaccharides and composed of 9 monosaccharides including Man, Gal, Rib, Rha, Fuc, Xyl, Ara, and GLC-UA.The monosaccharide molar ratio of each fraction was significantly different.Antioxidant analysis showed that NSP40 had the strongest ability to scavenge ABTS cationic radicals and hydroxyl radicals, while NSP60 had the strongest total reducing power.Therefore, gradient alcohol precipitation can apply to preliminary separate and obtain polysaccharide fractions with different physicochemical characteristics and biological activities.

Key words： Nannochloropsis; polysaccharides; gradient ethanol precipitation; physicochemical features; antioxidant activity

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