食品与发酵工业 >

2025 , Vol. 51 >Issue 4: 327 - 334

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.040067

分析与检测

二氧化锰包金纳米片介导免疫荧光传感器测定玉米面中呕吐毒素

  • 冯馨锐 ,
  • 王思佳 ,
  • 宋军澎 ,
  • 孙瑛楠 ,
  • 李晓娟 ,
  • 刘琰 ,
  • 彭帅
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  • 1(吉林医药学院 公共卫生学院,吉林 吉林,132013)
    2(吉林农业大学 食品科学与工程学院,吉林 长春,130118)
    3(青岛普瑞森医药科技有限公司,山东 青岛,266000)
第一作者:硕士研究生(刘琰讲师和彭帅讲师为共同通信作者,E-mail:jlu_liuyan@163.com;pengshuaiglx@163.com)

收稿日期: 2024-06-02

  修回日期: 2024-08-21

  网络出版日期: 2025-03-10

基金资助

吉林省重点研发项目(20210202109NC);吉林医药学院博士科研启动基金项目(2022JYBS011KJ)

收起

AuNP@MnO2based immunofluorescence sensor for determination of deoxynivalenol in corn flour

  • FENG Xinrui ,
  • WANG Sijia ,
  • SONG Junpeng ,
  • SUN Yingnan ,
  • LI Xiaojuan ,
  • LIU Yan ,
  • PENG Shuai
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  • 1(College of Public Health, Jilin Medical University, Jilin 132013, China)
    2(College of Food Science and Engineering, Jilin Agricultural University, Changchun 130118, China)
    3(Qingdao Preson Pharmaceutical Technology Co.Ltd., Qingdao 266000, China)

Received date: 2024-06-02

  Revised date: 2024-08-21

  Online published: 2025-03-10

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摘要

为了建立一种准确、快速、高效的呕吐毒素免疫荧光传感器(DON-icELISA),试验以呕吐毒素(deoxynivalenol,DON)为研究对象,利用二氧化锰包金纳米片(AuNP@MnO2)独特的光学特性作为荧光猝灭剂。结果表明,试验建立的DON-icELISA方法线性回归方程为Y=54.116X+110.53(R2=0.990 6)。检测范围(IC20~IC80)为0.021～0.273 ng/mL,检测限为0.013 ng/mL,且方法准确度较高。将所建立的DON-icELISA用于DON及其结构类似物3-脱氧雪腐镰刀菌烯醇(3-DON)、5-乙酰脱氧雪腐镰刀菌烯醇(5-DON)、黄曲霉毒素B1(aflatoxin B1,AFB1)、伏马菌素B1(fumonisin B1,FB1)、玉米赤霉烯酮(zearalenone,ZEN)、T-2毒素和赭曲霉毒素A(ochratoxin A,OTA)均无交叉反应。采用市售玉米面作为待检测食品,采用高(270 pg/mL)、中(150 pg/mL)、低(30 pg/mL)剂量进行加标回收测试该方法的回收率为94.74%～103.46%,平均变异系数为5.88%,通过3名不同专业试验人员进行加标回收试验,变异系数均小于5.00%,说明建立的DON-icELISA具有特异性好、敏感性高、操作简单且结果稳定的优势,可用于DON的快速筛检。

关键词： 呕吐毒素; 免疫荧光测定; 二氧化锰纳米片; 玉米面

本文引用格式

冯馨锐 , 王思佳 , 宋军澎 , 孙瑛楠 , 李晓娟 , 刘琰 , 彭帅 . 二氧化锰包金纳米片介导免疫荧光传感器测定玉米面中呕吐毒素[J]. 食品与发酵工业, 2025 , 51(4) : 327 -334 . DOI: 10.13995/j.cnki.11-1802/ts.040067

Abstract

This test focused on deoxynivalenol (DON) as the research object and utilized the unique optical properties of MnO2 nanosheets that wrapped around gold particles (AuNP@MnO2) as the fluorescence quencher.This study established an accurate, fast, and efficient DON immunofluorescence sensor (DON-icELISA).Results showed that the linear regression equation of the method established in this test was Y=54.116X+110.53 (R2=0.990 6).The detection range (IC20-IC80) was 0.021-0.273 ng/mL, and the detection limit was 0.013 ng/mL.The established DON-icELISA was applied to tests of DON and its structural analogues 3-DON, 5-DON, AFB1, FB1, ZEN, T-2, and OTA, without cross reactivity.Using commercially available corn flour as the food sample, the high (270 pg/mL), medium (150 pg/mL), and low (30 pg/mL) doses were used for the recovery test.The recovery rate of this method ranged from 94.74% to 103.46%, the average coefficient of variation was 5.88%.The coefficient of variation was less than 5.00% for the spiked recovery test conducted by three different professional testers.It indicated that DON-icELISA established in this test showed advantages of good specificity, high sensitivity, simple operation with stable results, and could be used for rapid screening of DON.

Key words： deoxynivalenol; immunofluorescence assay; MnO2 nanosheets; corn flour

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