2-苯乙醇是一种具有玫瑰香味的芳香族醇,被广泛应用于食品、日化和医药等领域中。该研究利用从发酵酱醪中筛选出的一株野生型生香酵母-贝酵母,通过生物转化方法探索了该生香酵母合成2-苯乙醇的能力。通过优化反应体系中葡萄糖、底物L-苯丙氨酸、无氨基酵母氮源及其他无机盐等成分构建生物转化体系,建立了适用于该生香酵母生产2-苯乙醇的转化方法。实验结果显示,该生香酵母转化48 h后2-苯乙醇的产量最高,为2.89 g/L。在此基础上,进一步优化了转化体系中碳源、氨基受体 α-酮戊二酸和离子混合营养液的浓度,2-苯乙醇的浓度提高了67%。最后,通过优化转化体系的pH、温度和溶氧等条件使得2-苯乙醇的质量浓度达到了6.05 g/L,较初始转化条件提高了109%。该方法所获2-苯乙醇不仅具有天然产物的属性和安全性,且其产量和转化率高,工艺也具有周期短和成本低的特点,具有很好的应用前景。
As a rose-scented aromatic alcohol, 2-phenylethanol has been widely used in the fields of food, daily chemicals, and medicine.In this study, a wild-type aroma-producing yeast (Saccharomyces bayanus) selected from fermented sauce mash was utilized to explore its ability to produce 2-phenylethanol through biotransformation methods.A biotransformation method suitable for producing 2-phenylethanol by S.bayanus was established by optimizing the ingredients of glucose, substrate L-phenylalanine, yeast nitrogen base, and other inorganic salts in the reaction system.It was shown that the highest titer of 2-phenylethanol was obtained after 48 h of transformation.On this basis, by further optimizing the concentrations of carbon source, amino acceptor of α-ketoglutaric acid as well as the mixed ionic nutrient solution, the titer of 2-phenylethanol was reached to 4.83 g/L, an increase of 67.13% compared to that of the initial conversion conditions. Finally, by optimizing the pH, temperature, and dissolved oxygen of the conversion system, the concentration of 2-phenylethanol reached 6.05 g/L, an increase of 109.34% compared to that of the initial conversion conditions.The 2-phenylethanol obtained by this method not only has good properties and the safety of natural products, but also has a high yield and conversion rate.In addition, the process also has the characteristics of short cycle and low cost.Therefore, this biological transformation method has good application prospects.
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