研究报告

茶多酚-卵白蛋白可食膜对冷鲜猪肉品质的影响及微生物生长模型的构建

  • 杨乐 ,
  • 刘丽莉 ,
  • 丁玥 ,
  • 程伟伟 ,
  • 肖枫
展开
  • 1(河南科技大学 食品与生物工程学院,河南 洛阳,471023)
    2(食品加工与安全国家级教学示范中心,河南 洛阳,471023)
    3(河南省食品加工与质量安全控制河南省国际联合实验室,河南 洛阳,471023)
    4(食品微生物河南省工程技术研究中心,河南 洛阳,471023)
第一作者:硕士研究生(刘丽莉教授为通信作者,E-mail:yangliuyilang@126.com)

收稿日期: 2024-02-29

  修回日期: 2024-04-23

  网络出版日期: 2025-04-14

基金资助

河南省重大科技攻关项目(221100110500);国家重点研发科技项目(2022YFF1101600);河南省科技攻关项目(242102110092);洛阳市科技攻关项目-公益项目(2101021A);河南科技大学校特聘教授科研经费项目(13510004)

Effect of tea polyphenol-ovalbumin edible film on quality of chilled pork and construction of microbial growth model

  • YANG Le ,
  • LIU Lili ,
  • DING Yue ,
  • CHENG Weiwei ,
  • XIAO Feng
Expand
  • 1(College of Food & Bioengineering, Henan University of Science and Technology, Luoyang 471023, China)
    2(National Demonstration Center for Experimental Food Processing and Safety Education, Luoyang 471023, China)
    3(Henan International Joint Laboratory of Food Processing and Quality and Safety Control, Luoyang 471023, China)
    4(Henan Engineering Technology Research Center of Food Microbiology, Luoyang 471023, China)

Received date: 2024-02-29

  Revised date: 2024-04-23

  Online published: 2025-04-14

摘要

为加强茶多酚(tea polyphenol,TP)与卵白蛋白(ovalbumin,OVA)在食品保鲜中的应用,该文针对猪肉货架期较短的缺点,将茶多酚与卵白蛋白进行复合,加入壳聚糖制备茶多酚-卵白蛋白可食膜液(TP-OVA)应用于猪肉保鲜,通过探究猪肉品质的变化研究可食膜的保鲜性能,建立猪肉中微生物的生长模型。结果表明,贮藏12 d后,2%(质量分数)TP膜液处理的猪肉pH显著低于空白组(P<0.05);差示扫描量热仪测定发现2%TP膜液处理的猪肉蛋白质变性温度最高,分别为58.16、81.17 ℃,热稳定性最好;色差与核磁测定结果说明2%TP膜液处理使猪肉具有最佳的保水性;2%TP膜液处理使得猪肉的质构特性显著优于其他组(P<0.05),且微观结构最佳。膜液处理组的菌落总数相较于空白组显著降低(P<0.05);修正的Gompertz模型能够较好地模拟微生物的生长情况,2%TP处理组的微生物最大生长速率最小,说明抑菌效果最佳。综上所述,TP添加量为2%膜液的抗氧化抑菌性能最佳,以期为开发天然高效的新型肉品包装提供新思路。

本文引用格式

杨乐 , 刘丽莉 , 丁玥 , 程伟伟 , 肖枫 . 茶多酚-卵白蛋白可食膜对冷鲜猪肉品质的影响及微生物生长模型的构建[J]. 食品与发酵工业, 2025 , 51(6) : 103 -111 . DOI: 10.13995/j.cnki.11-1802/ts.039020

Abstract

To strengthen the application of tea polyphenol (TP) and ovalbumin (OVA) in food preservation, this study, for the disadvantage of the short shelf life of pork, composited tea polyphenol with ovalbumin and added chitosan to prepare tea polyphenol-ovalbumin edible film liquid (TP-OVA) applied to pork preservation.The freshness performance of the edible film was investigated by exploring the changes in the quality of pork.Finally, the growth of microorganisms in pork was established.Results showed that after 12 days of storage, the pH of pork treated with 2% TP film solution was significantly lower than that of the blank group (P<0.05).The differential scanning calorimetry revealed that pork treated with 2% TP film solution had the highest protein denaturation temperatures of 58.16 ℃ and 81.17 ℃, respectively, which had the best thermal stability.The results of color difference and low field nuclear magnetic resonance measurements indicated that pork had the best water retention with the treatment of 2% TP film solution.The textural characteristics of pork were improved with the treatment of a 2% TP film solution.The 2% TP film solution treatment made the textural properties of pork significantly better than the other groups (P<0.05), and the microstructure was the best.The total number of colonies in the membrane treatment group was significantly lower than that in the blank group (P<0.05).The modified Gompertz model could better simulate the growth of microorganisms, and the maximum growth rate of microorganisms in the 2% TP treatment group was the smallest, which indicated that the bacterial inhibition effect was the best.In summary, the antioxidant and bacterial inhibition performance of the film solution with 2% TP addition was the best, to provide a new idea for the development of natural and efficient new meat packaging.

参考文献

[1] LI H, GUO H, LUO Q, et al.Current extraction, purification, and identification techniques of tea polyphenols:An updated review[J].Critical Reviews in Food Science and Nutrition, 2023, 63(19):3912-3930.
[2] 洪玉珠, 张佳乐, 张龙鑫, 等.玉米淀粉-卵白蛋白复合物替代小麦粉对纸杯蛋糕品质和消化特性的影响[J].食品工业科技, 2024, 45(10):77-83.
HONG Y Z, ZHANG J L, ZHANG L X, et al.Effects of corn starch-ovalbumin complex as substitution for wheat flour on the texture and digestive characteristics of cupcakes[J].Science and Technology of Food Industry, 2024, 45(10):77-83.
[3] YANG J, ZHAO Y J, SHAN B S, et al.Study on the interaction and functional properties of Dolichos lablab L.protein-tea polyphenols complexes[J].International Journal of Biological Macromolecules, 2023, 250:126006.
[4] CHEN Q, LIU Y H, LI Y, et al.Interaction and binding mechanism of ovalbumin with cereal phenolic acids:Improved structure, antioxidant activity, emulsifying and digestion properties for potential targeted delivery systems[J].Food Research International, 2024, 175:113726.
[5] 刘丽莉, 于影, 苏克楠, 等.植物多酚-牛血清白蛋白相互作用及对蛋白质结构的影响[J].农业工程学报, 2023, 39(13):290-298.
LIU L L, YU Y, SU K N, et al.Polyphenol-bovine serum albumin interaction and its influence on protein structure[J].Transactions of the Chinese Society of Agricultural Engineering, 2023, 39(13):290-298.
[6] RAHMA R A, PUTRI W D R, MEGATRI A A, et al.Optimisation of fish gelatin and red pitaya peel powder edible film production[J].International Journal of Food Science & Technology, 2023, 58(10):5578-5590.
[7] MOHAMMADI R, MOHAMMADIFAR M A, ROUHI M, et al.Physico-mechanical and structural properties of eggshell membrane gelatin- chitosan blend edible films[J].International Journal of Biological Macromolecules, 2018, 107:406-412.
[8] 董轶群, 罗鑫, 牛素敏, 等.复合保鲜剂对团头鲂尸僵过程中品质变化的影响[J].食品与发酵工业, 2022, 48(14):48-54.
DONG Y Q, LUO X, NIU S M, et al.Effect of compound preservative on quality of Megalobrama amblycephalala during rigor mortis[J].Food and Fermentation Industries, 2022, 48(14):48-54.
[9] SUTHARSAN J, BOYER C A, ZHAO J.Biological properties of chitosan edible films incorporated with different classes of flavonoids and their role in preserving the quality of chilled beef[J].Food Hydrocolloids, 2023, 139:108508.
[10] CHATURVEDI K, BASU S, SINGHA S, et al.Predictive microbial growth modelling for an effective shelf-life extension strategy of Chhana (Indian cottage cheese)[J].Food Control, 2023, 149:109697.
[11] 张金凤, 王艳文, 范恒军, 等.茶多酚-玉米胚芽粕蛋白膜制备及性能研究[J].粮食与饲料工业, 2023(3):46-51.
ZHANG J F, WANG Y W, FAN H J, et al.Preraration and property study of tea polyphenols and corn germ meal protein-membrane[J].Cereal & Feed Industry, 2023(3):46-51.
[12] 席小辉,胡瑜文,张升阳,等.肉豆蔻精油/羟丙基-β-环糊精包合物对冷鲜猪肉的保鲜效果[J].食品与发酵工业, 2024, 50(10):187-196.
XI X H, HU Y W, ZHANG S Y, et al.Preservation effect of nutmeg essential oil/HP-β-CD inclusion complex on chilled pork[J].Food and Fermentation Industries, 2024, 50(10):187-196.
[13] KONUK TAKMA D, KOREL F.Active packaging films as a carrier of black cumin essential oil:Development and effect on quality and shelf-life of chicken breast meat[J].Food Packaging and Shelf Life, 2019, 19:210-217.
[14] 陈婷婷, 崔泽恒, 包海蓉, 等.两种鱿鱼胴体肉热加工特性的研究[J].食品与发酵工业, 2023, 49(8):51-59.
CHEN T T, CUI Z H, BAO H R, et al.Study on thermal processing characteristics of two kinds of squid carcass meat[J].Food and Fermentation Industries, 2023, 49(8):51-59.
[15] KIM A N, LEE K Y, PARK C E, et al.Effect of heating under different vacuum levels on physicochemical and oxidative properties of beef sirloin[J].Foods, 2023, 12(7):1393.
[16] 罗程, 王缓, 陈银基, 等.魔芋胶对猪肉肌原纤维蛋白凝胶特性和保水特性的调控机制:基于相分离行为和水相稳定[J].中国农业科学, 2023, 56(9):1775-1786.
LUO C, WANG H, CHEN Y J, et al.The mechanism of effects of konjac gum on the gel property and water holding property of pork myofibrillar protein based on phase separation behavior and moisture stabilization[J].Scientia Agricultura Sinica, 2023, 56(9):1775-1786.
[17] 盖圣美, 游佳伟, 张雪娇, 等.利用低场核磁共振及成像技术鉴别注水肉糜[J].食品科学, 2020, 41(22):289-294.
GAI S M, YOU J W, ZHANG X J, et al.Discrimination of water-injected ground meat using low-field nuclear magnetic resonance and magnetic resonance imaging[J].Food Science, 2020, 41(22):289-294.
[18] 赵立, 邱慧敏, 廉嘉欣, 等.精氨酸低钠盐对猪肉品质的影响[J].中国食品学报, 2023, 23(11):137-146.
ZHAO L, QIU H M, LIAN J X, et al.Effects of arginine low-sodium salt on pork quality[J].Journal of Chinese Institute of Food Science and Technology, 2023, 23(11):137-146.
[19] 赵谋, 杨欢欢, 杨莉, 等.燕麦β-葡聚糖对低盐羊肉肉糜凝胶特性的影响[J].食品科学, 2023, 44(16):159-168.
ZHAO M, YANG H H, YANG L, et al.Effect of oat β-glucan on gel properties of low-salt mutton mince[J].Food Science, 2023, 44(16):159-168.
[20] WALID Y, MALGORZATA N, KATARZYNA R, et al.Effect of rosemary essential oil and ethanol extract on physicochemical and antibacterial properties of optimized gelatin-chitosan film using mixture design[J].Journal of Food Processing and Preservation, 2022, 46(1):e16059.
[21] KAYA G K.The effects of different packaging methods and sous vide cooking on chemical, sensory, and microbiological changes of marinated crayfish (Astacus leptodactylus Esch., 1823)[J].Journal of Food Processing and Preservation, 2022, 46(10):e16919.
[22] 伍梦婷, 郭娟娟, 许雄, 等.木薯变性淀粉对猪肉肌原纤维蛋白凝胶质构、流变及热力学特性的影响[J].食品工业科技, 2019, 40(8):49-53;60.
WU M T, GUO J J, XU X, et al.Effect of cassava modified starch on the gel textural, rheological and thermodynamic properties of pork myofibrillar protein[J].Science and Technology of Food Industry, 2019, 40(8):49-53;60.
[23] 张慧娟, 潘见.含CO的气调包装协同超高压对冷却猪肉杀菌护色效果的研究[J].食品研究与开发, 2021, 42(12):84-89.
ZHANG H J, PAN J.The synergistic effect of modified atmosphere packaging with CO and ultrahigh pressure on the sterilization and color protection of chilled pork[J].Food Research and Development, 2021, 42(12):84-89.
[24] 黄卉, 孙申宇, 魏涯, 等.红色肉贮藏期间肌红蛋白与肉色变化研究进展[J].南方水产科学, 2020, 16(3):119-124.
HUANG H, SUN S Y, WEI Y, et al.Research progress on color transformation of red meat during storage[J].South China Fisheries Science, 2020, 16(3):119-124.
[25] 周垚卿, 董静雯, 李学理, 等.茶多酚对不同肥瘦比猪肉的护色作用及差异机制研究[J].食品科技, 2020, 45(12):98-103.
ZHOU Y Q, DONG J W, LI X L, et al.Effect and mechanism of tea polyphenols on color of minced pork with different fat to muscle ratios[J].Food Science and Technology, 2020, 45(12):98-103.
[26] 李玲, 季慧, 康大成, 等.氧化条件下茶多酚对猪肉肌原纤维蛋白理化和凝胶特性的影响[J].食品科学, 2019, 40(2):12-17.
LI L, JI H, KANG D C, et al.Effect of tea polyphenols on physicochemical and gel properties of pork myofibrillar protein under oxidative conditions[J].Food Science, 2019, 40(2):12-17.
[27] 闫瑞, 唐敏, 陈东方, 等.白萝卜粉作为亚硝酸盐来源制备猪肉发酵干香肠[J].食品科学, 2023, 44(8):101-108.
YAN R, TANG M, CHEN D F, et al.Preparation of dry fermented pork sausage using radish powder as a source of nitrite[J].Food Science, 2023, 44(8):101-108.
[28] 周彬静, 刘小花, 彭菁, 等.荧光假单胞菌和热杀索丝菌对低温贮藏期间猪肉品质变化的影响[J].食品科学, 2022, 43(19):208-216.
ZHOU B J, LIU X H, PENG J, et al.Effects of Pseudomonas fluorescens and Brochothrix thermosphacta on quality changes of pork during low-temperature storage[J].Food Science, 2022, 43(19):208-216.
[29] 孙慧琳. 贮藏方式对牛肉的品质变化的影响[D].锦州:锦州医科大学, 2021:30-31.
SUN H L.Effect of storage methods on quality change of beef[D].Shenyang:Jinzhou Medical University, 2021:30-31.
文章导航

/