食品与发酵工业 >

2025 , Vol. 51 >Issue 7: 259 - 267

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.039588

研究报告

马尾藻多糖超声波辅助提取、结构表征及抗氧化活性研究

  • 栾翠荣 ,
  • 温南华 ,
  • 李致瑜 ,
  • 覃亮卓 ,
  • 蒋璇靓 ,
  • 刘淑集 ,
  • 杨贞标 ,
  • 杨杰 ,
  • 吴一晶
展开
  • 1(福建农林大学 未来技术学院(海峡联合研究院),福建 福州,350002)
    2(闽江学院 地理与海洋学院,福建 福州,350108)
    3(福建省海洋藻类活性物质制备与功能开发重点实验室,泉州师范学院,福建 泉州,362000)
    4(近海资源生物技术福建省高校重点实验室,泉州师范学院,福建 泉州,362000)
    5(福建省海洋生物增养殖与高值化利用重点实验室,福建省水产研究所,福建 厦门,361000)
第一作者:硕士研究生(杨杰副教授和吴一晶副教授为共同通信作者,E-mail:jie.yang@mju.edu.cn;yijingwu@mju.edu.cn)

收稿日期: 2024-04-17

  修回日期: 2024-05-16

  网络出版日期: 2025-04-29

基金资助

福建省海洋藻类活性物质制备与功能开发重点实验室开放课题(2022-KF07);福建省种业创新与产业化工程项目(2021FJSCZY01);国家自然科学基金青年科学基金项目(31801606)

收起

Ultrasonic-assisted extraction, structural characterization, and antioxidant activity of Sargassum polysaccharide

  • LUAN Cuirong ,
  • WEN Nanhua ,
  • LI Zhiyu ,
  • QIN Liangzhuo ,
  • JIANG Xuanjing ,
  • LIU Shuji ,
  • YANG Zhenbiao ,
  • YANG Jie ,
  • WU Yijing
Expand
  • 1(Haixia Institute of Science and Technology, School of Future Technology, Fujian Agriculture and Forestry University, Fuzhou 350002, China)
    2(College of Geography and Oceanography, Minjiang University, Fuzhou 350108, China)
    3(Fujian Province Key Laboratory for the Development of Bioactive Material from Marine Algae, Quanzhou Normal University, Quanzhou 362000, China)
    4(Key Laboratory of Inshore Resources Biotechnology Fujian Province University, Quanzhou Normal University, Quanzhou 362000, China)
    5(Key Laboratory of Marine Biomass Breeding and High-Value Utilization in Fujian Province, Fujian Fisheries Research Institute, Xiamen 361000, China)

Received date: 2024-04-17

  Revised date: 2024-05-16

  Online published: 2025-04-29

Fold

摘要

马尾藻(Sargassum)是我国常见的褐藻,具有多种生物活性,在功能食品领域中具有广阔的应用前景。该研究通过单因素试验优化超声辅助法提取马尾藻多糖的条件,同时利用配备示差折光检测器的多角度激光散射仪(size-exclusion chromatographic analysis with multiangle laser light scattering detection and refractive index detector,SEC-MALLS-RI)、离子色谱仪、傅里叶红外光谱仪、核磁共振仪等对最优条件得到的马尾藻多糖进行结构表征,并对其体外抗氧化活性进行探究。提取的最优条件为超声频率28 kHz、料液比1∶60(g∶mL)、提取温度70 ℃、提取时间180 min,在该条件下马尾藻多糖的提取率为9.73%。马尾藻多糖主要由果糖、糖醛酸、岩藻糖、半乳糖和甘露糖组成,其摩尔比为56.6∶16.2∶8.4∶8.3∶6.8。SEC-MALLS-RI表明多糖样品重均分子质量为1.18×107 (±3.13%)g/mol,并在溶液中高度分散。1H核磁共振谱分析表明,马尾藻多糖中糖苷键以β-型糖苷键为主。X射线衍射结果表明马尾藻多糖是一种半晶聚合物。通过扫描电子显微镜和原子力显微镜观察,多糖链倾向于聚合成纳米级片层状的均相结构。此外,马尾藻多糖具有较好的铁离子还原能力和超氧阴离子自由基清除能力,在一定质量浓度范围内呈现良好的剂量效应关系。该项研究表明,超声波辅助提取法能显著提高马尾藻多糖的得率,对马尾藻的高值化应用提供理论依据。

关键词： 马尾藻; 多糖; 结构表征; 抗氧化活性

本文引用格式

栾翠荣 , 温南华 , 李致瑜 , 覃亮卓 , 蒋璇靓 , 刘淑集 , 杨贞标 , 杨杰 , 吴一晶 . 马尾藻多糖超声波辅助提取、结构表征及抗氧化活性研究[J]. 食品与发酵工业, 2025 , 51(7) : 259 -267 . DOI: 10.13995/j.cnki.11-1802/ts.039588

Abstract

Sargassum is a common brown alga in China, which has many biological activities and broad application prospects in the fields of functional food.Ultrasonic-assisted extraction of Sargassum polysaccharide was optimized through single-factor experiments.Subsequently, the obtained polysaccharide was characterized by size-exclusion chromatographic analysis (SEC) coupled with multiangle laser light scattering (MALLS) detection and refractive index (RI) detector (SEC-MALLS-RI), ion chromatography, fourier transform infrared spectrometer, and nuclear magnetic resonance. The optimal ultrasound-assisted extraction conditions for Sargassum polysaccharide were as follows:ultrasonic frequency of 28 kHz, mass-liquid ratio of 1∶60 (g∶mL), extraction temperature of 70 ℃, and extraction time of 180 min.Under the optimal condition, the extraction rate was 9.73%, which was mainly composed of fructose, uronic acid, fucose, galactose, and mannose, with molar ratio of 56.6∶16.2∶8.4∶8.3∶6.8, respectively.SEC-MALLS-RI displayed that Sargassum polysaccharide was a highly dispersed macromolecule polymer with a molar weight of 1.18×107 (±3.13%) g/mol.On the other hand, according to 1H NMR spectra, the type of glycosidic linkage was mainly the β-glycosidic bond.Meanwhile, X-ray diffraction demonstrated Sargassum polysaccharide was a semi-crystalline polymer, while scanning electron microscope and atomic force microscope observation suggested polysaccharide chains were prone to be associated with weak and small aggregates with homogeneous morphology.Moreover, Sargassum polysaccharide showed desirable ferric ion-reducing capacity and free radical scavenging ability with a good dose-response relationship.This study demonstrated that the yield of Sargassum polysaccharide was effectively improved by the ultrasonic-assisted extraction method, providing a theoretical reference for the development and high-value utilization of Sargassum resources.

Key words： Sargassum; polysaccharide; structural characterization; antioxidant activities

参考文献

[1] ZENG F S, YAO Y F, WANG L F, et al.Polysaccharides as antioxidants and prooxidants in managing the double-edged sword of reactive oxygen species[J].Biomedicine & Pharmacotherapy, 2023, 159:114221.
[2] GUEHAZ K, BOUAL Z, ABDOU I, et al.Microalgae’s polysaccharides, are they potent antioxidants? Critical review[J].Archives of Microbiology, 2023, 206(1):14.
[3] ISMAIL G A, GHEDA S F, ABO-SHADY A M, et al.In vitro potential activity of some seaweeds as antioxidants and inhibitors of diabetic enzymes[J].Food Science and Technology, 2020, 40(3):681-691.
[4] LI C Y, WANG C S, WANG S F, et al.Optimization of ultrasonic-assisted extraction technology of Sargassum fusiforme polysaccharides and evaluation of their antioxidant activity[J].Food Science and Technology Research, 2013, 19(2):157-162.
[5] FAN X Q, XIAO X J, YU W, et al.Yucca schidigera purpurea-sourced arabinogalactan polysaccharides augments antioxidant capacity facilitating intestinal antioxidant functions[J].Carbohydrate Polymers, 2024, 326:121613.
[6] FANG R E, WEI Y J, FANG S Y, et al.Effects of Sargassum-derived oligosaccharides, polysaccharides and residues on ameliorating enteritis and dysbiosis in a murine model of food allergy[J].Journal of Functional Foods, 2023, 110:105844.
[7] FAN S R, YU G Q, NIE W J, et al.Antitumor activity and underlying mechanism of Sargassum fusiforme polysaccharides in CNE-bearing mice[J].International Journal of Biological Macromolecules, 2018, 112:516-522.
[8] 袁慧情, 胡佳敏, 刘思溢, 等.马尾藻多糖拮抗LPS诱导的巨噬细胞极化及铁死亡[J].中国免疫学杂志, 2023, 39(10):2034-2040.
YUAN H Q, HU J M, LIU S Y, et al.Sargassum polysaccharides antagonize LPS-induced macrophage polarization and ferroptosis[J].Chinese Journal of Immunology, 2023, 39(10):2034-2040.
[9] JIANG H, YANG S Q, CHAKKA V P, et al.Purification and biological activities of enzymatically degraded Sargassum fusiforme polysaccharides[J].Chemistry & Biodiversity, 2021, 18(3):e2000930.
[10] WEN C T, ZHANG J X, ZHANG H H, et al.Plant protein-derived antioxidant peptides:Isolation, identification, mechanism of action and application in food systems:A review[J].Trends in Food Science & Technology, 2020, 105:308-322.
[11] ABDELHEDI O, NASRI R, SOUISSI N, et al.Sulfated polysaccharides from common smooth hound:Extraction and assessment of anti-ACE, antioxidant and antibacterial activities[J].Carbohydrate Polymers, 2016, 152:605-614.
[12] WEI Q, ZHANG Y H.Ultrasound-assisted polysaccharide extraction from Cercis chinensis and properites, antioxidant activity of polysaccharide[J].Ultrasonics Sonochemistry, 2023, 96:106422.
[13] OTERO P, CARPENA M, GARCIA-OLIVEIRA P, et al.Seaweed polysaccharides:Emerging extraction technologies, chemical modifications and bioactive properties[J].Critical Reviews in Food Science and Nutrition, 2023, 63(13):1901-1929.
[14] PEÑALVER R, LORENZO J M, NIETO G.Bioaccessibility, digestibility and nutritional properties of algae and Cyanophyceae as basis of their potential as functional food ingredients[J].Applied Food Research, 2024, 4(1):100404.
[15] YANG T H, YAO H T, CHIANG M T.Red algae (Gelidium amansii) reduces adiposity via activation of lipolysis in rats with diabetes induced by streptozotocin-nicotinamide[J].Journal of Food and Drug Analysis, 2015, 23(4):758-765.
[16] CHEN L, CHEN J S, WU K G, et al.Improved low pH emulsification properties of glycated peanut protein isolate by ultrasound Maillard reaction[J].Journal of Agricultural and Food Chemistry, 2016, 64(27):5531-5538.
[17] GUO L N, KONG N, ZHANG X Y, et al.Multimode ultrasonic extraction of polysaccharides from maca (Lepidium meyenii):Optimization, purification, and in vitro immunoregulatory activity[J].Ultrasonics Sonochemistry, 2022, 88:106062.
[18] WELCH E C, CHALTAS K, TRIPATHI A.Ultrasound frequency sonication facilitates high-throughput and uniform dissociation of cellular aggregates and tissues[J].SLAS Technology, 2023, 28(2):70-81.
[19] 梁杰, 邓波, 刘涛, 等.响应面法优化坛紫菜抗氧化肽酶法制备工艺[J].食品研究与开发, 2020, 41(5):1-6.
LIANG J, DENG B, LIU T, et al.Preparation of antioxidant peptides from Porphyra haitanensis via response surface optimization[J].Food Research and Development, 2020, 41(5):1-6.
[20] CHEN P L, YOU Q X, LI X, et al.Polysaccharide fractions from Fortunella margarita affect proliferation of Bifidobacterium adolescentis ATCC 15703 and undergo structural changes following fermentation[J].International Journal of Biological Macromolecules, 2019, 123:1070-1078.
[21] LIU X G, BIAN J, LI D Q, et al.Structural features, antioxidant and acetylcholinesterase inhibitory activities of polysaccharides from stem of Physalis alkekengi L[J].Industrial Crops and Products, 2019, 129:654-661.
[22] SHI H M, LI J C, YU J, et al.Extraction, purification and antioxidant activity of polysaccharides from different parts of Hibiscus manihot L[J].Journal of Molecular Structure, 2024, 1295:136598.
[23] YANG M, ZHOU D Q, XIAO H, et al.Marine-derived uronic acid-containing polysaccharides:Structures, sources, production, and nutritional functions[J].Trends in Food Science & Technology, 2022, 122:1-12.
[24] 何坤明, 王国锭, 白新鹏, 等.山茱萸籽多糖分离纯化、结构表征及抗氧化活性[J].食品科学, 2021, 42(19):81-88.
HE K M, WANG G D, BAI X P, et al.Isolation, purification, structure characterization and antioxidant activity of Cornus officinalis seed polysaccharides[J].Food Science, 2021, 42(19):81-88.
[25] CAO W, WU J P, ZHAO X Y, et al.Structural elucidation of an active polysaccharide from Radix Puerariae lobatae and its protection against acute alcoholic liver disease[J].Carbohydrate Polymers, 2024, 325:121565.
[26] 王青芬, 敖新宇, 宫树森, 等.海巴戟果实多糖的结构特征及体外抗氧化活性研究[J].林产化学与工业, 2023, 43(6):81-88.
WANG Q F, AO X Y, GONG S S, et al.Structural characteristics and antioxidant activity of polysaccharide from Morinda citrifolia fruit in vitro[J].Chemistry and Industry of Forest Products, 2023, 43(6):81-88.
[27] JRIDI M, MEZHOUDI M, ABDELHEDI O, et al.Bioactive potential and structural characterization of sulfated polysaccharides from Bullet tuna (Auxis Rochei) by-products[J].Carbohydrate Polymers, 2018, 194:319-327.
[28] 蒋茂婷, 冉艳红, 刘娜, 等.蒜皮水溶性多糖的制备及结构表征[J].食品科学, 2022, 43(6):57-65.
JIANG M T, RAN Y H, LIU N, et al.Preparation and structural characterization of water-soluble polysaccharide from garlic skin[J].Food Science, 2022, 43(6):57-65.
[29] NEP E I, CARNACHAN S M, NGWULUKA N C, et al.Structural characterisation and rheological properties of a polysaccharide from sesame leaves (Sesamum radiatum Schumach.& Thonn.)[J].Carbohydrate Polymers, 2016, 152:541-547.
[30] 刘鹏, 李静静, 乔彦良, 等.黄芩多糖的提取及结构表征[J].中国畜牧兽医, 2023, 50(6):2518-2530.
LIU P, LI J J, QIAO Y L, et al.Extraction and structure characterization of Scutellaria baicalensis polysaccharide[J].China Animal Husbandry & Veterinary Medicine, 2023, 50(6):2518-2530.
[31] MEDELEANU M A, HĂDĂRUGĂ D I, MUNTEAN C V, et al.Structure-property relationships on recrystallized β-cyclodextrin solvates:A focus on X-ray diffractometry, FTIR and thermal analyses[J].Carbohydrate Polymers, 2021, 265:118079.
[32] BUTT H S, ULRIKSEN E S, RISE F, et al.Structural elucidation of novel pro-inflammatory polysaccharides from Daphne mezereum L[J].Carbohydrate Polymers, 2024, 324:121554.
[33] WANG S N, ZHAO H K, QU D N, et al.Destruction of hydrogen bonding and electrostatic interaction in soy hull polysaccharide:Effect on emulsion stability[J].Food Hydrocolloids, 2022, 124:107304.
[34] WANG L B, LI L Y, GAO J Y, et al.Characterization, antioxidant and immunomodulatory effects of selenized polysaccharides from dandelion roots[J].Carbohydrate Polymers, 2021, 260:117796.
[35] HUANG H L, TSAI I L, LIN C, et al.Intelligent films of marine polysaccharides and purple cauliflower extract for food packaging and spoilage monitoring[J].Carbohydrate Polymers, 2023, 299:120133.
[36] WANG Q, HAO L, ZHANG A Q, et al.Extraction and characterization of polysaccharides from Schisandra sphenanthera fruit by Lactobacillus plantarum CICC 23121-assisted fermentation[J].International Journal of Biological Macromolecules, 2024, 259:129135.
[37] HOLUBOVÁ M, LOBAZ V, LOUKOTOVÁ L, et al.Does polysaccharide glycogen behave as a promoter of amyloid fibril formation at physiologically relevant concentrations?[J].Soft Matter, 2021, 17(6):1628-1641.
[38] SANTO K P, FABIJANIC K I, CHENG C Y, et al.Modeling of the effects of metal complexation on the morphology and rheology of xanthan gum polysaccharide solutions[J].Macromolecules, 2021, 54(18):8675-8692.
[39] DONG R H, HUANG Z Y, MA W J, et al.Fabrication of nano complexes for anthocyanins delivery by ovalbumin and differently dense sulphate half-ester polysaccharides nanocarriers:Enhanced stability, bio-accessibility, and antioxidant properties[J].Food Chemistry, 2024, 432:137263.
[40] CADO G, KERDJOUDJ H, CHASSEPOT A, et al.Polysaccharide films built by simultaneous or alternate spray:A rapid way to engineer biomaterial surfaces[J].Langmuir, 2012, 28(22):8470-8478.
[41] LIU R, YAN Z, CHU F X, et al.Comparative study on chain conformations, physicochemical and rheological properties of three acidic polysaccharides from Opuntia dillenii Haw.fruits[J].International Journal of Biological Macromolecules, 2024, 262:130027.
[42] WU Y J, XU X, JIANG X J, et al.Valorisation of harmful algae bloom (Enteromorpha prolifera) for polysaccharide and crude bio-oil production[J].Fuel, 2022, 324:124482.
[43] ZHAO L, WANG L, GUO Z M, et al.Polysaccharides from Pogostemon cablin (blanco) Benth.:Characterization and antioxidant activities[J].Frontiers in Pharmacology, 2022, 13:933669.
[44] 李学秀, 刘海棠, 安永贞, 等.桉木预水解液中半纤维素多糖分离纯化及抗氧化活性研究[J].中国造纸, 2022, 41(4):30-37.
LI X X, LIU H T, AN Y Z, et al.Study on separation, purification and antioxidant activities of hemicellulose polysaccharides from Eucalyptus pre-hydrolysate[J].China Pulp & Paper, 2022, 41(4):30-37.
[45] ZHOU S Y, HUANG G L, HUANG H L.Extraction, derivatization and antioxidant activities of onion polysaccharide[J].Food Chemistry, 2022, 388:133000.
[46] JAYASREE SUBHASH A, BABATUNDE BAMIGBADE G, AL-RAMADI B, et al.Characterizing date seed polysaccharides:A comprehensive study on extraction, biological activities, prebiotic potential, gut microbiota modulation, and rheology using microwave-assisted deep eutectic solvent[J].Food Chemistry, 2024, 444:138618.
[47] ZHANG L Y, WANG S R, ZHANG W M, et al.Prospects of yam (Dioscorea) polysaccharides:Structural features, bioactivities and applications[J].Food Chemistry, 2024, 446:138897.
[48] YANG J J, ZHANG X, DAI J F, et al.Effect of fermentation modification on the physicochemical characteristics and anti-aging related activities of Polygonatum kingianum polysaccharides[J].International Journal of Biological Macromolecules, 2023, 235:123661.
Options
文章导航

/

〈 〉

技术支持：北京玛格泰克科技发展有限公司