食品与发酵工业 >

2025 , Vol. 51 >Issue 9: 84 - 90

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.039779

研究报告

不同盐浓度下单增李斯特菌和副溶血性弧菌混合生物被膜的形成

  • 李婷 ,
  • 陈清莹 ,
  • 黄铭新 ,
  • 张睿 ,
  • 陈梦璐 ,
  • 钟青萍
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  • (广东省食品质量安全重点实验室,华南农业大学 食品学院,广东 广州,510642)
第一作者:硕士研究生(钟青萍教授为通信作者,E-mail:zhongqp@scau.edu.cn)

收稿日期: 2024-05-06

  修回日期: 2024-05-20

  网络出版日期: 2025-05-28

基金资助

广东省基础与应用基础研究基金项目(2024A1515012695,2021A1515011083);国家级大学生创新创业训练计划项目(202410564031);国家自然科学基金面上项目(31972046);广东省科技计划项目(2020B1212060059)

收起

The mixed biofilm formation of Listeria monocytogenes and Vibrio parahaemolyticus at different salt concentrations

  • LI Ting ,
  • CHEN Qingying ,
  • HUANG Mingxin ,
  • ZHANG Rui ,
  • CHEN Menglu ,
  • ZHONG Qingping
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  • (Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou 510642, China)

Received date: 2024-05-06

  Revised date: 2024-05-20

  Online published: 2025-05-28

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摘要

该研究旨在探究不同NaCl浓度对单增李斯特菌和副溶血性弧菌混合生物被膜形成的影响。测定了不同NaCl浓度下单种和混合生物被膜的生物量、代谢活性,以及生物被膜和浮游菌的细胞组成,分析了两菌的相互作用。结果表明,与单种生物被膜相比,混合生物被膜的形成能力和代谢活性较强;在0.5 g/100 mL NaCl浓度的胰酪大豆胨液体培养基中,优势菌株为单增李斯特菌;而在1~5 g/100 mL NaCl浓度的混合培养体系中,副溶血性弧菌是优势菌株,单增李斯特菌对副溶血性弧菌生物被膜形成具有促进作用,而副溶血性弧菌对单增李斯特菌具有抑制作用,且在1.5 g/100 mL NaCl浓度下两者相互作用最强。综上,副溶血性弧菌是优势菌株,在混合生物被膜中占主导地位。研究结果为控制单增李斯特菌和副溶血性弧菌混合生物被膜污染提供理论依据。

关键词： 单增李斯特菌; 副溶血性弧菌; 混合培养; 氯化钠; 生物被膜

本文引用格式

李婷 , 陈清莹 , 黄铭新 , 张睿 , 陈梦璐 , 钟青萍 . 不同盐浓度下单增李斯特菌和副溶血性弧菌混合生物被膜的形成[J]. 食品与发酵工业, 2025 , 51(9) : 84 -90 . DOI: 10.13995/j.cnki.11-1802/ts.039779

Abstract

The purpose of this study was to investigate the effects of different concentrations of sodium chloride on the mixed biofilm formation of Listeria monocytogenes and Vibrio parahaemolyticus.The biomasses and metabolic activities of single species and mixed biofilm, the cell composition of biofilm and plankton at different NaCl concentrations were determined, and the interaction between the two bacteria was analyzed.The results showed that the mixed biofilm exhibited stronger biofilm formation and metabolic activity compared to the single biofilm.The dominant strain in the mixed culture system was L.monocytogenes in the TSB medium with 0.5 g/100 mL NaCl, while V.parahaemolyticus dominated in the mixed culture system at 1-5 g/100mL NaCl.L.monocytogenes showed promotion effects on the formation of V.parahaemolyticus biofilm, and V.parahaemolyticus inhibited that of L.monocytogenes.The interaction between the two bacteria was strongest at 1.5 g/100 mL NaCl concentration.In general, V.parahaemolyticus was the dominant strain in the mixed biofilm.This study provides scientific data for the control of mixed biofilm contamination of L.monocytogenes and V.parahaemolyticus.

Key words： Listeria monocytogenes; Vibrio parahaemolyticus; mixed culture; sodium chloride; biofilm

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