正畸治疗是改善牙齿排列和咬合关系的有效手段,但治疗过程中常常伴随着正畸患者牙槽骨的过度骨吸收,而中间普雷沃氏菌(Prevotella intermedia)是影响牙槽骨骨吸收的关键微生物。因此,通过抑制P.intermedia在正畸患者口腔中的定植,可以预防正畸患者牙槽骨骨吸收的发生。该研究首先在体外验证了植物乳植杆菌CCFM8724对P.intermedia生物膜的抑制效果。随后使用植物乳植杆菌CCFM8724干预正畸模型大鼠,通过检测大鼠骨代谢指标、牙槽骨病理组织、大鼠口腔P.intermedia的定植量和牙龈组织炎症因子的表达水平来评估植物乳植杆菌CCFM8724的干预效果。结果表明,植物乳植杆菌CCFM8724显著提高了正畸模型大鼠第一磨牙根尖处BV/TV值(P<0.01),抑制了牙周组织肿瘤坏死因子α和白介素-6的表达,同时促进了血清骨保护素(osteoprotegerin,OPG)的上调(P<0.01)。相较于模型组,植物乳植杆菌CCFM8724还显著降低了P.intermedia的定植量(P<0.05)。因此,植物乳植杆菌CCFM8724可通过抑制P.intermedia在大鼠口腔中的定植,下调正畸及致病菌诱导的牙周炎症反应,提高OPG/RANKL/RANK通路中OPG和RANKL的比例水平,缓解大鼠牙槽骨正畸诱导性骨吸收,该研究结果可为正畸诱导性骨吸收的临床治疗提供一种新的策略。
Orthodontic treatment represents an effective modality for the amelioration of dental alignment and occlusal relationships.However, this process is frequently accompanied by excessive resorption of the alveolar bone in orthodontic patients.Prevotella intermedia has been identified as a pivotal microorganism influencing alveolar bone resorption.Consequently, the prevention of P.intermedia colonization in the oral cavity of orthodontic patients may serve to forestall the onset of alveolar bone resorption.This study initially validated the inhibitory effects of Lactiplantibacillus plantarum CCFM8724 on P.intermedia biofilms in vitro.Subsequently, the modulatory effects of L.plantarum CCFM8724 were investigated in orthodontic model rats.The interventions were assessed by monitoring bone metabolism indicators, histopathology of the alveolar bone, colonization levels of P.intermedia in the rat oral cavity, and the expression levels of gingival tissue inflammatory factors.The results showed that L.plantarum CCFM8724 significantly increased the bone volume/tissue volume (BV/TV) ratio at root apex of the first molar in rats (P<0.01), suppressed the expression of TNF-α and IL-6 in periodontal tissue, and upregulated osteoprotegerin (OPG) expression in serum (P<0.01).Furthermore, compared to the model group, P.intermedia colonization was significantly reduced following administration of L.plantarum CCFM8724 (P<0.05).These findings suggested that L.plantarum CCFM8724 can mitigate orthodontic-induced alveolar bone resorption in rats by inhibiting P.intermedia colonization in the oral cavity, downregulating orthodontic and pathogenic microbe-induced periodontal inflammation, and increasing the ratio of OPG to receptor activator of nuclear factor-kappa B ligand (RANKL) in the OPG/RANKL/RANK pathway.The study provides novel insights into the clinical management of orthodontically induced alveolar bone resorption.
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