食品与发酵工业 >

2025 , Vol. 51 >Issue 8: 276 - 283

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.038910

研究报告

阿拉伯半乳聚糖处理对青脆李果实采后糖酸代谢和能量代谢的调控作用研究

  • 刘莹 ,
  • 袁田 ,
  • 刘玲 ,
  • 韩赛影 ,
  • 郑安祺 ,
  • 张婷婷 ,
  • 明建 ,
  • 邓丽莉
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  • 1(西南大学 食品科学学院,重庆,400715)
    2(食品科学与工程国家级实验教学示范中心(西南大学),重庆,400715)
第一作者:本科生(邓丽莉副教授为通信作者,E-mail:denglili_361@163.com)

收稿日期: 2024-02-20

  修回日期: 2024-05-17

  网络出版日期: 2025-05-28

基金资助

农产综函【2022】35号;西南大学大学生创新训练项目(X202310635141)

收起

Effect of arabinogalactan treatment on sugar acid metabolism and energy metabolism of postharvest Qingcui plum

  • LIU Ying ,
  • YUAN Tian ,
  • LIU Ling ,
  • HAN Saiying ,
  • ZHENG Anqi ,
  • ZHANG Tingting ,
  • MING Jian ,
  • DENG Lili
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  • 1(College of Food Science, Southwest University, Chongqing 400715, China)
    2(National Demonstration Center for Experimental Food Science and Technology Education (Southwest University), Chongqing 400715, China)

Received date: 2024-02-20

  Revised date: 2024-05-17

  Online published: 2025-05-28

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摘要

为研究阿拉伯半乳聚糖(arabinogalactan, AG)浸泡处理对青脆李果实成熟衰老进程的调控作用,该实验用5 g/L AG对采后青脆李果实进行浸泡处理,通过对贮藏果实可溶性糖、有机酸、能量物质(ATP、ADP、AMP以及能荷)和烟酰胺腺嘌呤二核苷酸(nicotinamide adenine dinucleotide, NAD)、NADPH、呼吸代谢相关酶的活性,以及相关基因表达量的分析,初步从糖酸代谢和能量代谢的角度阐明AG处理延缓采后青脆李果实成熟衰老的机制。结果表明,AG处理可延缓采后青脆李果实可溶性糖和有机酸的减少,并能促进贮藏后期果实ATP含量的上升,抑制果实能荷的下降;此外,AG处理可降低青脆李果实的NAD含量,同时减慢NADPH降低的速度。同时,AG处理能使与呼吸代谢有关的酶的基因表达量降低,进而显著降低果实丙酮酸脱羧酶、乙醇脱氢酶、磷酸果糖激酶的活性(P＜0.05)。综上,AG处理主要通过改变呼吸代谢途径,减少果实有机物的消耗,以此维持青脆李果实内部较高的糖酸含量和能量供应,从而延缓青脆李果实的成熟衰老进程,延长果实的贮藏期和货架期。

关键词： 青脆李; 阿拉伯半乳聚糖; 采后; 呼吸代谢; 能量

本文引用格式

刘莹 , 袁田 , 刘玲 , 韩赛影 , 郑安祺 , 张婷婷 , 明建 , 邓丽莉 . 阿拉伯半乳聚糖处理对青脆李果实采后糖酸代谢和能量代谢的调控作用研究[J]. 食品与发酵工业, 2025 , 51(8) : 276 -283 . DOI: 10.13995/j.cnki.11-1802/ts.038910

Abstract

To investigate the regulatory mechanism of arabinogalactan (AG) immersion treatment on the ripening and aging process of Qingcui plum, this experiment used 5 g/L AG to soak the harvested Qingcui plum, the contents of soluble sugars, organic acids, energy substances (ATP, ADP, AMP, and energy charge), NAD, NADPH, and activities of respiratory-related enzymes, combined with the expression levels of related genes were determined.Results showed that AG treatment could delay the decreased rate of soluble sugars and organic acids in Qingcui plum, promote the increase of ATP content, and inhibit the decrease of fruit energy charge value.In addition, AG treatment could reduce the NAD content of Qingcui plum and slow down the rate of NADPH reduction.Meanwhile, AG treatment could reduce the gene expression levels of enzymes related to respiratory metabolism, thereby significantly reducing the activities of fruit pyruvate decarboxylase, alcohol dehydrogenase, and 6-phosphofructokinase (P<0.05).Based on this, it was believed that AG treatment mainly changed the respiratory metabolic pathway, reduced the consumption of organic matter in the fruit, and maintained a high sugar and acid content and energy supply inside the Qingcui plum, thus delaying the ripening and aging process of the fruit and extending the storage and shelf life of the fruit.

Key words： Qingcui plum; arabinogalactan; postharvest; respiratory metabolism; energy

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