传统抑菌剂的泛用使细菌表现出日趋严重的耐药性,以群体感应系统为靶点的新型抑菌剂具有不使细菌产生耐药性的优势。该研究旨在为开发天然产物作为新型抑菌剂提供理论支持。以沙门氏菌为受试菌株,测定铁皮、叠鞘石斛提取物对沙门氏菌的最小抑菌浓度(minimum inhibitory concentration,MIC),通过生长曲线实验、泳动实验和生物膜实验研究提取物对沙门氏菌生长状况、运动能力和生物膜生成能力的影响,通过HPLC分析提取物中的主要成分,通过分子对接模拟探究提取物对沙门氏菌群体感应系统的作用机制。结果显示,铁皮石斛提取物的MIC为8 mg/mL,叠鞘石斛提取物的MIC为4 mg/mL,在亚抑菌浓度下,沙门氏菌的生长不受影响,但运动能力和产生物膜能力均受到抑制(P＜0.05),抑制效果呈现剂量依赖性,2种提取物均含有异鼠李素、金丝桃苷、槲皮素这3种单体成分,活性单体均能与LuxS蛋白相结合,提示提取物通过活性单体与受体蛋白结合来抑制致病因子表达实现抑菌作用。
蒋璐遥
,
周俊含
,
严阳
,
陈杰
,
邓晓东
,
舒馨
,
唐崇邵
,
罗莹欣
,
赵志伟
,
魏玉颖
,
甘菊丽
,
杜彦锋
,
燕文柏
,
张薇薇
. 两种石斛提取物对沙门氏菌群体感应的影响及机制研究[J]. 食品与发酵工业, 2025
, 51(11)
: 75
-80
.
DOI: 10.13995/j.cnki.11-1802/ts.039425
Traditional antibacterials are still widely used, but this has caused bacteria to develop considerable antibiotic resistance.One benefit of the new antibacterials that target the quorum sensing mechanism is that they don’t cause drug resistance in bacteria.This work offers theoretical justification for the creation of novel antibacterials from natural materials.Salmonella is the test strain used in this study.The MIC of two Dendrobium extracts against Salmonella was investigated.Using in swimming, growth curve, and biofilm experiments to investigate the impact of the extract on Salmonella motility, growth, and biofilm formation.Utilizing HPLC to examine the extract’s primary constituents.We employ the molecular docking simulation to investigate the mechanism of the extract on the Salmonella quorum sensing system.The MIC of Dendrobium officinale Kimura extracts on Salmonella is 8 mg/mL and the MIC of Dendrobium denneanum Kerr extracts on Salmonella is 4 mg/mL.The extracts exhibit concentration dependence and significantly restrict Salmonella’s ability to migrate, form biofilms, and swarm at subinhibitory doses.The HPLC analysis reveals the presence of isorhamnetin, hyperin, and quercetin in both Dendrobium extracts.Both LuxS proteins are able to bind to these active monomers.These findings suggest that via binding monomeric components to matching proteins, the extract inhibited the production of pathogenic factors, hence having an antibacterial impact.
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