食品与发酵工业 >

2025 , Vol. 51 >Issue 12: 84 - 91

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.040201

研究报告

可代谢半乳糖嗜热链球菌IMAU20153Y的诱变选育及其特性分析

  • 卢纹慧 ,
  • 邰佳慧 ,
  • 吴娜 ,
  • 刘金惠 ,
  • 丹彤
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  • (内蒙古农业大学,乳品生物技术与工程教育部重点实验室,农业农村部奶制品加工重点实验室内蒙古自治区乳品生物技术与工程重点实验室,内蒙古 呼和浩特,010018)
第一作者:硕士研究生(丹彤副教授为通信作者,E-mail:dantong813218@aliyun.com)

收稿日期: 2024-06-17

  修回日期: 2024-09-06

  网络出版日期: 2025-07-11

基金资助

国家自然科学基金项目(32072235);内蒙古自然科学基金面上项目(2022MS03013)

收起

Mutagenic selection of metabolizable galactose Streptococcus thermophilus IMAU20153Y and its characterization

  • LU Wenhui ,
  • TAI Jiahui ,
  • WU Na ,
  • LIU Jinhui ,
  • DAN Tong
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  • (Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Key Laboratory of Dairy Products Processing, Ministry of Agriculture and Rural Affairs, Inner Mongolia Key Laboratory of Dairy Biotechnology and Engineering, Inner Mongolia Agricultural University, Hohhot 010018, China)

Received date: 2024-06-17

  Revised date: 2024-09-06

  Online published: 2025-07-11

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摘要

嗜热链球菌(Streptococcus thermophilus)是一种常用的发酵剂菌株,目前在发酵乳制品中得到了广泛的应用。大多数S.thermophilus不具备半乳糖代谢能力,导致发酵乳中半乳糖含量较高。因此,可采用化学诱变技术处理S.thermophilus,使其能够代谢半乳糖,开发一种低半乳糖含量的发酵乳。该研究以一株发酵特性优良的S.thermophilus IMAU20153为研究对象,采用亚硝基胍(N-methyl-N′-nitro-N-nitrosoguanidine,NTG)对其诱变。通过分析和半乳糖代谢相关酶的活力、检测发酵时间以及进行基因序列比对,筛选出可代谢半乳糖的诱变S.thermophilus IMAU20153Y。通过HPLC进行分析,结果表明,S.thermophilus IMAU20153Y在发酵和贮藏过程中能够代谢更多的乳糖和半乳糖,产生乳酸。该研究的顺利进行,为低半乳糖发酵乳制品的研发及应用提供了新思路。

关键词： 嗜热链球菌; 亚硝基胍; 诱变; 半乳糖

本文引用格式

卢纹慧 , 邰佳慧 , 吴娜 , 刘金惠 , 丹彤 . 可代谢半乳糖嗜热链球菌IMAU20153Y的诱变选育及其特性分析[J]. 食品与发酵工业, 2025 , 51(12) : 84 -91 . DOI: 10.13995/j.cnki.11-1802/ts.040201

Abstract

Streptococcus thermophilus is an important starter culture widely used in dairy fermentation.Most S.thermophilus strains originating from milk can not use the galactose moiety of lactose, resulting in high galactose content in fermented milk.In this study, S.thermophilus IMAU20153 which was known to have good fermentation characteristics, was treated by using the chemical mutagen agent N-methyl-N′-nitro-N-nitrosoguanidine (NTG), which can be metabolize galactose, and used for the industrial production of fermented milk with low levels of galactose.S.thermophilus IMAU20153Y, which can metabolize galactose, was screened by comparing the enzyme activity assays, fermentation time and sequencing.HPLC analysis demonstrated that S.thermophilus IMAU20153Y enhanced ability to utilize lactose and galactose, leading to increased lactic acid.These findings offer new insights on the development and application of fermented milk with low levels of galactose.

Key words： Streptococcus thermophilus; N-methyl-N′-nitro-N-nitrosoguanidine; mutant; galactose

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