为了研究pH响应多酚递送系统的抗炎活性,通过玉米醇溶蛋白(zein)和碳量子点(carbon quantum dots, CQDs)复合纳米颗粒负载褐藻多酚(phlorotannin, PTN)构建zein-PTN-CQDs纳米颗粒,并在此基础上添加单宁酸和Fe3+制备zein-PTN-CQDs-FeⅢ纳米颗粒。通过多酚体外释放、细胞毒性评估、细胞凋亡成像观察、抗炎和活性氧检测实验,探究纳米颗粒的pH响应释放特性和抗炎活性。结果表明,加入CQDs可以有效降低纳米粒子的粒径并提高包埋率。单宁酸和Fe3+能够在zein-PTN-CQDs纳米颗粒表面形成具有pH响应性能的多酚-金属包覆网络。zein-PTN-CQD-FeⅢ纳米颗粒在pH值为6.2、5、4的酸性条件下释放率分别为78%、89%和96%,显著高于中性条件下的释放率(28%),呈现出持续释放和pH响应的特性。对于脂多糖刺激RAW 264.7细胞,zein-PTN-CQDs-FeⅢ纳米颗粒能显著降低活性氧的产生,并且对促炎因子(IL-1β、IL-6和TNF-α)的抑制能力和抗炎因子(IL-10和前列腺素E2)的促进能力优于PTN组和zein-CQDs-FeⅢ组。
To investigate the anti-inflammatory activity of pH-responsive polyphenol delivery systems, zein-phlorotannin (PTN)-carbon quantum dots (CQDs) nanoparticles were constructed by loading PTN into zein-CQDs nanoparticles.Zein-PTN-CQDs-FeⅢ nanoparticles were further prepared by adding tannic acid and Fe3+ based on zein-PTN-CQDs nanoparticles.The pH-responsive release characteristics and anti-inflammatory activity of the nanoparticles were explored through experiments on polyphenol release in vitro, cell toxicity, cell apoptosis imaging, anti-inflammatory properties, and reactive oxygen species (ROS) detection.Results showed that the addition of CQDs effectively reduced the particle size and increased the encapsulation efficiency.pH-responsive polyphenol-metal coating networks were formed on the surface of zein-PTN-CQDs nanoparticles through the combination of tannic acid and Fe3+.The release rates of PTN-CQDs-FeIII nanoparticles under acidic conditions of pH 6.2, 5, and 4 were 78%, 89%, and 96%, respectively, significantly higher than those under neutral conditions (28%), exhibiting sustained release and pH-responsive characteristics.For lipopolysaccharide-stimulated RAW 264.7 cells, zein-PTN-CQDs-FeⅢ nanoparticles significantly reduced ROS production and exhibited superior inhibition of pro-inflammatory factors (IL-1β, IL-6, and TNF-α) and promotion of anti-inflammatory factors (IL-10 and prostaglandin E2) compared to the PTN group and zein-CQDs-FeⅢ group.
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