食品与发酵工业 >

2025 , Vol. 51 >Issue 12: 132 - 142

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.040470

研究报告

姜黄素与酪蛋白-海藻酸钠相互作用及复合物性质研究

  • 秦娟娟 ,
  • 杨敏 ,
  • 陈欣 ,
  • 柯得倩
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  • 1(甘肃农业大学 动物医学院,甘肃 兰州,730070)
    2(甘肃农业大学 理学院,甘肃 兰州,730070)
第一作者:博士研究生(杨敏教授为通信作者,E-mail:yummy12@163.com)

收稿日期: 2024-07-12

  修回日期: 2024-09-05

  网络出版日期: 2025-07-11

基金资助

研究生“创新之星”项目(2023CXZX-633);甘肃省重点研发计划(23YFNA0017);2024年创新驱动助力工程项目(GXH20240328-15);兰州市人才创新创业项目(2023-RC-22)

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Interaction between curcumin and casein-sodium alginate and properties of their complexes

  • QIN Juanjuan ,
  • YANG Min ,
  • CHEN Xin ,
  • KE Deqian
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  • 1(College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China)
    2(College of Science, Gansu Agricultural University, Lanzhou 730070, China)

Received date: 2024-07-12

  Revised date: 2024-09-05

  Online published: 2025-07-11

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摘要

该文以酪蛋白(casein,CN)和海藻酸钠(sodium alginate,SA)为基质,采用荧光分析法解析了CN、CN-SA与姜黄素(curcumin,Cur)的相互作用机理;通过喷雾干燥制备了酪蛋白-姜黄素(CN-Cur)、酪蛋白-海藻酸钠-姜黄素(CN-SA-Cur)复合物微胶囊,分析了复合物的性质,并探究了复合物的抗氧化性。结果表明,相比于单一CN,CN-SA对Cur具有更高的结合亲和性,且CN与SA体积比为5∶5时,结合常数均可达104 L/mol,与Cur主要以范德华力、氢键结合。SA的添加降低了CN-Cur复合物微胶囊表面的褶皱程度;当CN与SA体积比为5∶5~4∶6时形成的复合物为粒度均匀的微米球形颗粒。CN-SA-Cur复合物相较于CN-Cur具有更高的热稳定性。此外,SA的添加改善了CN-Cur的溶解性和乳化性。经CN-SA负载后,Cur的ABTS阳离子自由基清除能力显著提高。研究结果可为CN-SA复合体系在Cur递送中的应用提供参考依据。

关键词： 姜黄素; 酪蛋白; 海藻酸钠; 结合常数; 抗氧化性

本文引用格式

秦娟娟 , 杨敏 , 陈欣 , 柯得倩 . 姜黄素与酪蛋白-海藻酸钠相互作用及复合物性质研究[J]. 食品与发酵工业, 2025 , 51(12) : 132 -142 . DOI: 10.13995/j.cnki.11-1802/ts.040470

Abstract

In this paper, casein (CN) and sodium alginate (SA) were chosen as matrix and the interaction between CN or CN-SA and curcumin (Cur) was analyzed by fluorescence analysis.The microcapsules of casein-curcumin (CN-Cur) and casein-sodium alginate-curcumin (CN-SA-Cur) complexes were prepared by spray drying.The properties of the complexes were analyzed, and the antioxidant properties of the complexes were investigated.Results showed that compared with the single CN, CN-SA had a higher binding affinity for Cur, and when the volume ratio of CN to SA was 5∶5, the binding constant could reach 104 L/mol, and CN-SA was bound to Cur by van der Waals force and hydrogen bond mainly.The addition of SA reduced the degree of wrinkles on the surface of the CN-Cur microcapsules.When the volume ratio of CN to SA was 5∶5-4∶6, the complexes existed uniform micron spherical particles.The CN-SA-Cur composite had higher thermal stability than CN-Cur.In addition, the addition of SA improved the solubility and emulsification of CN-Cur.The ABTS cationic radical scavenging activity of Cur was significantly enhanced by CN-SA loading.The results of this study could provide references for the application of CN-SA composite system in Cur delivery.

Key words： curcumin; casein; sodium alginate; binding constant; oxidation resistance

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