研究报告

植物乳植杆菌KULG13对发酵肉块产生宣威火腿风味的影响

  • 吴昕颖 ,
  • 臧立萍 ,
  • 徐鸿挺 ,
  • 曹建新 ,
  • 张基亮
展开
  • 1(昆明理工大学 食品科学与工程学院,云南 昆明,650504)
    2(宣威市永进食品有限公司,云南 宣威,655400)
第一作者:硕士研究生(张基亮讲师和曹建新教授为共同通信作者,E-mail:zhangjl2348@163.com;jxcao321@hotmail.com)

收稿日期: 2024-06-22

  修回日期: 2024-08-13

  网络出版日期: 2025-07-11

基金资助

云南省重大科技项目(202202AG050009)

Effect of Lactobacillus plantarum KULG13 on flavor of Xuanwei ham produced by fermented meat

  • WU Xinying ,
  • ZANG Liping ,
  • XU Hongting ,
  • CAO Jianxin ,
  • ZHANG Jiliang
Expand
  • 1(Faculty of Food Science and Engineering, Kunming University of Science and Technology, Kunming 650504, China)
    2(Xuanwei Yongjin Food Co.Ltd., Xuanwei 655400, China)

Received date: 2024-06-22

  Revised date: 2024-08-13

  Online published: 2025-07-11

摘要

为了打破火腿自然发酵带来的批次间风味、质地和色泽等的差异,该研究从宣威优质火腿中筛选乳酸菌,评价其对发酵肉块的风味和感官的影响。植物乳植杆菌KULG13,可以显著地提高肉块的a*值、同时降低肉块的水分活度、提高肉块硬度、减缓负面风味化合物的形成以及提高肉类风味化合物的浓度(P<0.05)。对干腌肉块整体风味起显著贡献作用的挥发性化合物有13种,包括7种醛类、2种醇类、1种酯类、2种酮类和1种呋喃类。KULG13发酵肉块与优质宣威火腿相比,其风味物质含量和物质种类相似度为79.60%和46.55%,结合感官评价的结果,KULG13最有可能作为产生宣威火腿风味的潜在发酵剂。

本文引用格式

吴昕颖 , 臧立萍 , 徐鸿挺 , 曹建新 , 张基亮 . 植物乳植杆菌KULG13对发酵肉块产生宣威火腿风味的影响[J]. 食品与发酵工业, 2025 , 51(12) : 199 -206 . DOI: 10.13995/j.cnki.11-1802/ts.040260

Abstract

To break the difference in flavor, texture, and color between batches caused by naturally fermented hams, lactic acid bacteria were screened from Xuanwei high-quality ham and evaluated their effect on the flavor and sensory quality of fermented meat.Lactobacillus plantarum KULG13 could significantly increase the a* value of meat, decrease the water activity, increase the hardness of meat, reduce the formation of negative flavor compounds, and increase the concentration of meat flavor compounds (P<0.05) at the same time.There were 13 volatile compounds, including 7 aldehydes, 1 ester, 2 ketones, and 1 furan, which contributed significantly to the overall flavor of fermented meat.Compared with high-quality Xuanwei ham, KULG13 fermented meat had 79.60% and 46.55% similarity in the flavor substance content and species.Combined with the results of sensory evaluation, KULG13 was the most likely a potential starter culture for Xuanwei ham.

参考文献

[1] DE L AGÜERO N, FRIZZO L S, OUWEHAND A C, et al.Technological characterisation of probiotic lactic acid bacteria as starter cultures for dry fermented sausages[J].Foods, 2020, 9(5):596.
[2] CANDOGAN K, KARTIKA S, WARDLAW F B, et al.Type of bacterial starter culture, aging and fermentation effects on some characteristics of inoculated beef sausages[J].European Food Research and Technology, 2008, 227(6):1651-1661.
[3] 刘柳, 刘学文, 张鑫燚.发酵肉传统制作工艺参数优化研究[J].食品工业科技, 2008, 29(1):202-204.
LIU L, LIU X W, ZHANG X Y.Study on optimization of traditional processing parameters of fermented meat[J].Science and Technology of Food Industry, 2008, 29(1):202-204.
[4] PETROVA I, AASEN I M, RUSTAD T, et al.Manufacture of dry-cured ham:A review.Part 1.Biochemical changes during the technological process[J].European Food Research and Technology, 2015, 241(5):587-599.
[5] 孔维严. 宣威火腿工艺及当代发展概况调查报告[D].重庆:重庆师范大学, 2016.
KONG W Y.Investigation report on Xuanwei ham technology and its contemporary development[D].Chongqing:Chongqing Normal University, 2016.
[6] LI C L, MORA L, GALLEGO M, et al.Evaluation of main post-translational modifications occurring in naturally generated peptides during the ripening of Spanish dry-cured ham[J].Food Chemistry, 2020, 332:127388.
[7] SUN Q X, CHEN Q, LI F F, et al.Biogenic amine inhibition and quality protection of Harbin dry sausages by inoculation with Staphylococcus xylosus and Lactobacillus plantarum[J].Food Control, 2016, 68:358-366.
[8] HUGHES M C, KERRY J P, ARENDT E K, et al.Characterization of proteolysis during the ripening of semi-dry fermented sausages[J].Meat Science, 2002, 62(2):205-216.
[9] CHEN Q, KONG B H, SUN Q X, et al.Antioxidant potential of a unique LAB culture isolated from Harbin dry sausage:in vitro and in a sausage model[J].Meat Science, 2015, 110:180-188.
[10] 米瑞芳, 陈曦, 戚彪, 等.乳杆菌发酵剂对酸肉挥发性风味成分的影响[J].肉类研究, 2018, 32(4):48-55.
MI R F, CHEN X, QI B, et al.Effect of Lactobacillus starter cultures on the volatile flavor components of sour meat[J].Meat Research, 2018, 32(4):48-55.
[11] 吴双慧, 杨梓垚, 牛茵, 等.复配发酵剂对发酵香肠的品质及挥发性风味的影响[J].食品工业科技, 2024, 45(5):126-133.
WU S H, YANG Z Y, NIU Y, et al.Effects of mixed starter culture on quality and volatile flavor of fermented sausages[J].Science and Technology of Food Industry, 2024, 45(5):126-133.
[12] LIU R, MA Y, CHEN L, et al.Effects of the addition of leucine on flavor and quality of sausage fermented by Lactobacillus fermentum YZU-06 and Staphylococcus saprophyticus CGMCC 3475[J].Frontiers in Microbiology, 2022, 13:1118907.
[13] 孙庆申, 王钰涵, 韩德权, 等.酸菜中具有抑菌活性乳酸菌的分离鉴定及其产细菌素特性[J].食品科学技术学报, 2022, 40(4):64-73.
SUN Q S, WANG Y H, HAN D Q, et al.Isolation and identification of lactic acid bacteria with antibacterial activity in pickles and their bacteriocin production characteristics[J].Journal of Food Science and Technology, 2022, 40(4):64-73.
[14] 蒋云升, 潘明, 汪志君, 等.如皋火腿用霉菌性表面涂膜发酵剂的应用研究[J].食品与发酵工业, 2007, 33(7):81-84.
JIANG Y S, PAN M, WANG Z J, et al.The study of application of fermentation on the surface of Rugao ham[J].Food and Fermentation Industries, 2007, 33(7):81-84.
[15] 李佳林, 梁超, 黄青.均匀交变磁场对鲫鱼肉冷冻保鲜效果的影响[J].安徽大学学报(自然科学版), 2023, 47(6):37-41.
LI J L, LIANG C, HUANG Q.Effect of uniform alternating magnetic field on freezing and freshness-keeping of crucian carp meat[J].Journal of Anhui University (Natural Science Edition), 2023, 47(6):37-41.
[16] WĘSIERSKA E, PALKA K, BOGDAÉSKA J, et al.Sensory quality of selected raw ripened meat products[J].Acta Scientiarum Polonorum.Technologia Alimentaria, 2013, 12(1):41-50.
[17] TOLEDANO A, JORDANO R, LÓPEZ C, et al.Proteolytic activity of lactic acid bacteria strains and fungal biota for potential use as starter cultures in dry-cured ham[J].Journal of Food Protection, 2011, 74(5):826-829.
[18] LI C, ZOU Y L, LIAO G Z, et al.Identification of characteristic flavor compounds and small molecule metabolites during the ripening process of Nuodeng ham by GC-IMS, GC–MS combined with metabolomics[J].Food Chemistry, 2024, 440:138188.
[19] VERPLANKEN K, WAUTERS J, VERCRUYSSE V, et al.Sensory evaluation of boar-taint-containing minced meat, dry-cured ham and dry fermented sausage by a trained expert panel and consumers[J].Food Chemistry, 2017, 233:247-255.
[20] 包媛媛, 林奇, 黄艾祥.传统宣威火腿的加工工艺[J].肉类工业, 2007(7):16-18.
BAO Y Y, LIN Q, HUANG A X.Processing technology of traditional Xuan-Wei ham[J].Meat Industry, 2007(7):16-18.
[21] HU Y Y, LI Y J, LI X G, et al.Application of lactic acid bacteria for improving the quality of reduced-salt dry fermented sausage:Texture, color, and flavor profiles[J].LWT, 2022, 154:112723.
[22] KATO T, MATSUDA T, TAHARA T, et al.Effects of meat-conditioning and lactic fermentation on pork muscle protein degradation[J].Bioscience, Biotechnology, and Biochemistry, 1994, 58(2):408-410.
[23] WANG D X, CHENG F, WANG Y, et al.The changes occurring in proteins during processing and storage of fermented meat products and their regulation by lactic acid bacteria[J].Foods, 2022, 11(16):2427.
[24] DU H Z, CHEN Q, LIU Q, et al.Evaluation of flavor characteristics of bacon smoked with different woodchips by HS-SPME-GC-MS combined with an electronic tongue and electronic nose[J].Meat Science, 2021, 182:108626.
[25] WANG J, AZIZ T, BAI R X, et al.Dynamic change of bacterial diversity, metabolic pathways, and flavor during ripening of the Chinese fermented sausage[J].Frontiers in Microbiology, 2022, 13:990606.
[26] LIU D Y, BAI L, FENG X, et al.Characterization of Jinhua ham aroma profiles in specific to aging time by gas chromatography-ion mobility spectrometry (GC-IMS)[J].Meat Science, 2020, 168:108178.
文章导航

/