研究报告

本土非酿酒酵母对混合发酵威代尔冰酒风味及品质影响的比较分析

  • 易子程 ,
  • 常腾文 ,
  • 司慧宁 ,
  • 陈翔 ,
  • 闫玥 ,
  • 开建荣 ,
  • 彭帮柱 ,
  • 葛谦
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  • 1(宁夏农产品质量标准与检测技术研究所,宁夏 银川,750002)
    2(华中农业大学 食品科学技术学院,湖北 武汉,430000)
    3(银川市林业(园林)有害生物检疫检验站,宁夏 银川,750021)
第一作者:硕士研究生(葛谦副研究员为通信作者,E-mail:278842005@qq.com)

收稿日期: 2024-05-22

  修回日期: 2024-08-05

  网络出版日期: 2025-07-11

基金资助

宁夏回族自治区重点研发计划项目(2023BCF01025);自治区农业高质量发展和生态保护科技创新示范课题(NGSB-2021-5-04);宁夏农林科学院先导资金项目(NKYJ-23-02)

Comparative analysis of impact of local non-Saccharomyces yeasts on flavor and quality of mixed-fermented Vidal ice wine

  • YI Zicheng ,
  • CHANG Tengwen ,
  • SI Huining ,
  • CHEN Xiang ,
  • YAN Yue ,
  • KAI Jianrong ,
  • PENG Bangzhu ,
  • GE Qian
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  • 1(Institute of Agricultural Product Quality Standards and Testing Technology, Yinchuan 750002, China)
    2(College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430000, China)
    3(Yinchuan City Forestry (Landscape) Harmful Organism Quarantine and Inspection Station, Yinchuan 750021, China)

Received date: 2024-05-22

  Revised date: 2024-08-05

  Online published: 2025-07-11

摘要

为研究本土不同非酿酒酵母与酿酒酵母混合发酵对贺兰山东麓威代尔冰葡萄酒风味品质的影响,该试验选用仙人掌有孢汉逊酵母(HO52)、葡萄汁有孢汉逊酵母(HU181)和费比恩塞伯林德纳氏酵母(CF20)等9株自筛的本土非酿酒酵母,分别与酿酒酵母按照1∶1接种比例混合发酵,并以酿酒酵母单一发酵为对照。结果显示,与对照组相比,大部分混合发酵处理均能有效控制冰酒中有机酸浓度,提升多酚含量,增强香气复杂性。其中,相较于其他非酿酒酵母,仙人掌有孢汉逊酵母(HO52)能较好地提升冰酒中酚类化合物的含量,如3-羟基肉桂酸、槲皮素及龙胆酸的浓度均有显著增加。进一步分析发现,该酵母混菌发酵的酒样在香气成分方面具有独特性,其中高级醇、酯类及萜烯类物质的含量较其他非酿酒酵母发酵酒样分别增加了68.4%、56.1%和34.2%。感官品评结果显示,仙人掌有孢汉逊酵母(HO52)混菌所酿冰酒中热带水果、花香和甜味尤为丰富,这一特性与己酸乙酯、月桂酸乙酯、异戊醇和β-大马士酮等香气物质的含量增加密切相关。总体而言,仙人掌有孢汉逊酵母(HO52)不仅能赋予冰酒浓郁而纯正的香气特征,较好地保留威代尔冰葡萄酒的特征风味,也增强了香气的复杂性和层次感,有效提升了冰酒的感官品质。

本文引用格式

易子程 , 常腾文 , 司慧宁 , 陈翔 , 闫玥 , 开建荣 , 彭帮柱 , 葛谦 . 本土非酿酒酵母对混合发酵威代尔冰酒风味及品质影响的比较分析[J]. 食品与发酵工业, 2025 , 51(12) : 215 -225 . DOI: 10.13995/j.cnki.11-1802/ts.039966

Abstract

To investigate the impact of mixed fermentation with different local non-Saccharomyces yeasts and Saccharomyces yeasts on the flavor quality of Vidal ice wine from the eastern foothills of Helan Mountain, this study selected nine strains of local non-Saccharomyces yeasts, including Hanseniaspora opuntiae (HO52), Hanseniaspora uvarum (HU181), and Cyberlindnera fabianii (CF20).These were mixed with Saccharomyces yeasts in a 1∶1 inoculation ratio for fermentation, with Saccharomyces yeasts-only fermentation serving as the control.The results indicated that compared to the control group, most mixed fermentation treatments effectively controlled the concentration of organic acids in the ice wine, enhanced the polyphenol content, and increased aromatic complexity.Among the non-Saccharomyces yeasts, Hanseniaspora opuntiae (HO52) notably improved the content of phenolic compounds in the ice wine, such as significantly higher concentrations of 3-hydroxycinnamic acid, quercetin, and 2,5-dihydroxybenzoic acid.Further analysis revealed that the wine samples fermented with this yeast mixture were unique in their aromatic components, with increases of 68.4% in higher alcohols, 56.1% in esters, and 34.2% in terpenes compared to other non-Saccharomyces yeasts fermentations.Sensory evaluation showed that the ice wine brewed with Hanseniaspora opuntiae (HO52) was rich in tropical fruits, floral notes, and sweetness.This characteristic correlates closely with increased concentrations of ethyl caproate, ethyl laurate, 3-methyl-1-butanol, and β-damascenone.Overall, Hanseniaspora opuntiae (HO52) not only imparts a rich and authentic aroma to the ice wine but also retains the characteristic flavor of Vidal ice wine, enhancing the complexity and layering of aromas and effectively improving the sensory quality of the ice wine.

参考文献

[1] CHIDI B S, MAFATA M, NOTSHOKOVU N Z, et al.Impact of grape temperature at pressing on organic acids and oenological characteristics of methode cap classique wines[J].South African Journal of Enology and Viticulture, 2018, 39(1):106-115.
[2] 马玥, 唐柯, 徐岩, 等.固相萃取结合GC-O/MS分析威代尔冰葡萄酒中的香气活性化合物[J].食品与发酵工业, 2015,41(4):153-159.
MA Y, TANG K, XU Y, et al.Solid-phase extraction-GC-O/MS for the analysis of odor-active volatile compounds in Vidal icewine[J].Food and Fermentation Industries, 2015, 41(4):153-159.
[3] HUANG L, MA Y, TIAN X, et al.Chemosensory characteristics of regional Vidal icewines from China and Canada[J].Food Chemistry, 2018, 261:66-74.
[4] 卜光明, 周化斌, 周茂洪, 等.酿造酒中非酿酒酵母的研究进展[J].食品工业科技, 2019, 40(14):346-352.
BU G M, ZHOU H B, ZHOU M H, et al.Research progress on the non-Saccharomyces in the brewing wine fermentation[J].Science and Technology of Food Industry, 2019, 40(14):346-352.
[5] TUFARIELLO M, FRAGASSO M, PICO J, et al.Influence of non-Saccharomyces on wine chemistry:A focus on aroma-related compounds[J].Molecules, 2021, 26(3):644.
[6] ZHANG B Q, SHEN J Y, DUAN C Q, et al.Use of indigenous Hanseniaspora vineae and Metschnikowia pulcherrima co-fermentation with Saccharomyces cerevisiae to improve the aroma diversity of Vidal Blanc icewine[J].Frontiers in Microbiology, 2018, 9:2303.
[7] JIANG X H, LU Y Y, LIU S Q.Effects of different yeasts on physicochemical and oenological properties of red dragon fruit wine fermented with Saccharomyces cerevisiae, Torulaspora delbrueckii and Lachancea thermotolerans[J].Microorganisms, 2020, 8(3):153.
[8] SUN Y, LI E H, QI X T, et al.Changes of diversity and population of yeasts during the fermentations by pure and mixed inoculation of Saccharomyces cerevisiae strains[J].Annals of Microbiology, 2015, 65(2):911-919.
[9] LIN M M H, BOSS P K, WALKER M E, et al.Evaluation of indigenous non-Saccharomyces yeasts isolated from a South Australian vineyard for their potential as wine starter cultures[J].International Journal of Food Microbiology, 2020, 312:108373.
[10] HU K, JIN G J, MEI W C, et al.Increase of medium-chain fatty acid ethyl ester content in mixed H.uvarum/S.cerevisiae fermentation leads to wine fruity aroma enhancement[J].Food Chemistry, 2018, 239:495-501.
[11] 郑海宏, 罗华, 侯红萍.葡萄醋发酵工艺研究及其有机酸组分分析[J].中国酿造, 2019, 38(2):139-143.
ZHENG H H, LUO H, HOU H P.Fermentation technology of grape vinegar and analysis of its organic acid components[J].China Brewing, 2019, 38(2):139-143.
[12] WEI J P, ZHANG Y X, QIU Y, et al.Chemical composition, sensorial properties, and aroma-active compounds of ciders fermented with Hanseniaspora osmophila and Torulaspora quercuum in co- and sequential fermentations[J].Food Chemistry, 2020, 306:125623.
[13] PRIOR K J, BAUER F F, DIVOL B.The utilisation of nitrogenous compounds by commercial non-Saccharomyces yeasts associated with wine[J].Food Microbiology, 2019, 79:75-84.
[14] 田秀, 许引虎, 李惠琳, 等.产硫化氢酿酒酵母菌株对黑比诺干红葡萄酒品质的影响[J].食品与发酵工业, 2018, 44(5):115-124.
TIAN X, XU Y H, LI H L, et al.Effects of Saccharomyces cerevisiae strains producing hydrogen sulfide on the quality of Pinot Noir dry red wine[J].Food and Fermentation Industries, 2018, 44(5):115-124.
[15] CONTRERAS A, HIDALGO C, SCHMIDT S, et al.The application of non-Saccharomyces yeast in fermentations with limited aeration as a strategy for the production of wine with reduced alcohol content[J].International Journal of Food Microbiology, 2015, 205:7-15.
[16] BALMASEDA A, ROZÈS N, BORDONS A, et al.Torulaspora delbrueckii promotes malolactic fermentation in high polyphenolic red wines[J].LWT, 2021,148:111777.
[17] 唐柯, 王蓓, 马玥, 等.不同酵母与温度发酵的威代尔冰葡萄酒有机酸分析[J].食品与发酵工业, 2015,41(8):153-158.
TANG K, WANG B, MA Y, et al.Analysis of organic acids in Vidal ice wine fermented with different species of yeast strains at different temperatures[J].Food and Fermentation Industries, 2015, 41(8):153-158.
[18] 曾竟蓝, 马胤鹏, 秦丹, 等.果酒中有机酸的作用及检测方法研究[J].中国酿造, 2018, 37(6):183-187.
ZENG J L, MA Y P, QIN D, et al.Research on effect and detection method of organic acids in fruit wine[J].China Brewing, 2018, 37(6):183-187.
[19] 刘晓燕, 张小月, 阿卜杜喀依尔·阿卜杜艾尼, 等.混菌发酵、酒泥陈酿结合β-葡聚糖酶对赤霞珠干红葡萄酒有机酸含量的影响[J].食品与发酵工业, 2022, 48(15):55-61.
LIU X Y, ZHANG X Y, ABUDUOAINI A, et al.Effects of mixed fermentation,wine lees aging combined with β-glucanase on organic acid content of Cabernet Sauvignon dry red wine[J].Food and Fermentation Industries, 2022, 48(15):55-61.
[20] 邓海霞, 郭晨晨, 李二虎.酿酒酵母乙酸代谢调控机制及低产菌株选育的研究进展[J].食品科学, 2023,44(13):183-192.
DENG H X, GUO C C, LI E H.Research progress on the regulation mechanism of acetic acid metabolism in Saccharomyces cerevisiae and breeding of low acetic acid-producing strains[J].Food Science, 2023, 44(13):183-192.
[21] 张欣珂, 赵旭, 成池芳, 等.葡萄酒中的酚类物质Ⅰ:种类、结构及其检测方法研究进展[J].食品科学, 2019, 40(15):255-268.
ZHANG X K, ZHAO X, CHENG C F, et al.Phenolics in wines Ⅰ:A review of categories, structures and detection methods[J].Food Science, 2019, 40(15):255-268.
[22] 万宁静, 尹鼎泽, 王平, 等.不同生态区摩尔多瓦葡萄酒的风味物质及感官特征解析[J].食品与发酵工业, 2025,51(5):299-309.
WAN N J, YIN D Z, WANG P, et al.Analysis of flavor compounds and sensory characteristics of Moldovan wines from different ecological zones[J].Food and Fermentation Industries, 2025,51(5):299-309.
[23] SADOUDI M, TOURDOT-MARÉCHAL R, ROUSSEAUX S, et al.Yeast-yeast interactions revealed by aromatic profile analysis of Sauvignon Blanc wine fermented by single or co-culture of non-Saccharomyces and Saccharomyces yeasts[J].Food Microbiology, 2012, 32(2):243-253.
[24] VICENTE J, RUIZ J, TOMASI S, et al.Impact of rare yeasts in Saccharomyces cerevisiae wine fermentation performance:Population prevalence and growth phenotype of Cyberlindnera fabianii, Kazachstania unispora, and Naganishia globosa[J].Food Microbiology, 2023, 110:104189.
[25] 张文静, 杨诗妮, 杜爽, 等.本土毕赤克鲁维酵母与酿酒酵母混合发酵葡萄酒的增香潜力分析[J].食品科学, 2020, 41(12):84-90.
ZHANG W J, YANG S N, DU S, et al.Potential application of mixed starter cultures of indigenous Pichia kluyveri and Saccharomyces cerevisiae to wine aroma enhancement[J].Food Science, 2020, 41(12):84-90.
[26] LIU P T, LU L, DUAN C Q, et al.The contribution of indigenous non-Saccharomyces wine yeast to improved aromatic quality of Cabernet Sauvignon wines by spontaneous fermentation[J].LWT, 2016, 71:356-363.
[27] 申静云, 刘沛通, 段长青, 等.不同有孢汉逊酵母与酿酒酵母混合发酵对威代尔冰葡萄酒香气的影响[J].食品与发酵工业, 2017, 43(10):16-23.
SHEN J Y, LIU P T, DUAN C Q, et al.Effects of mixed fermentation by different Hanseniaspora genus yeasts and Saccharomyces cerevisiae on the aroma compounds in Vidal ice wine[J].Food and Fermentation Industries, 2017, 43(10):16-23.
[28] 李俊娥, 毛亚玲, 祝霞, 等.苹果酸-乳酸发酵接种方式对赤霞珠干红葡萄酒香气品质的影响[J].食品与发酵工业, 2021, 47(19):126-136.
LI J E, MAO Y L, ZHU X, et al.Effects of different inoculation on aroma quality of Cabernet Sauvignon dry red wine[J].Food and Fermentation Industries, 2021, 47(19):126-136.
[29] MOREIRA N, MENDES F, GUEDES DE PINHO P, et al.Heavy sulphur compounds, higher alcohols and esters production profile of Hanseniaspora uvarum and Hanseniaspora guilliermondii grown as pure and mixed cultures in grape must[J].International Journal of Food Microbiology, 2008, 124(3):231-238.
[30] VIANA F, GIL J V, VALLÉS S, et al.Increasing the levels of 2-phenylethyl acetate in wine through the use of a mixed culture of Hanseniaspora osmophila and Saccharomyces cerevisiae[J].International Journal of Food Microbiology, 2009, 135(1):68-74.
[31] 梁丽红, 高娉娉, 张馨文, 等.优选本土非酿酒酵母混合发酵在干红葡萄酒中的应用潜力分析[J].食品与发酵工业, 2022, 48(14):118-124.
LIANG L H, GAO P P, ZHANG X W, et al.Potential application of mixed fermentation of superior indigenous non-Saccharomyces cerevisiae on dry red wine[J].Food and Fermentation Industries, 2022, 48(14):118-124.
[32] 赵美, 田秀, 李敏, 等.粟酒裂殖酵母与酿酒酵母共同接种发酵对‘黑比诺’干红葡萄酒品质的影响[J].食品科学, 2021, 42(24):108-116.
ZHAO M, TIAN X, LI M, et al.Effect of mixed culture fermentation with Schizosaccharomyces pombe and Saccharomyces cerevisiae on the quality of ‘Pinot Noir’ dry red wine[J].Food Science, 2021, 42(24):108-116.
[33] 谭凤玲, 王宝石, 胡培霞, 等.非酿酒酵母在葡萄酒混菌发酵中的应用及其挑战[J].食品与发酵工业, 2020, 46(22):282-286.
TAN F L, WANG B S, HU P X, et al.Application and challenge of non-Saccharomyces cerevisiae yeast in the mixed fermentation of fruit wineA[J].Food and Fermentation Industries, 2020, 46(22):282-286.
[34] 胡瑞祺, 杨薇熹, 凌梦琪, 等.贺兰山东麓产区地块间‘赤霞珠’和‘马瑟兰’葡萄酒香气差异分析[J].中国酿造, 2024, 43(6):49-58.
HU R Q, YANG W X, LING M Q, et al.Analysis of aroma difference between ‘Cabernet Sauvignon’ and ‘Marselan’ wines among parcels in the Eastern Foothills of Helan Mountain[J].China Brewing, 2024, 43(6):49-58.
[35] 唐柯, 马玥, 徐岩, 等.冰葡萄酒重要风味化合物嗅觉阈值的研究[J].食品与发酵工业, 2016, 42(1):148-151.
TANG K, MA Y, XU Y, et al.Study on olfactory thresholds for several flavor components in ice wine[J].Food and Fermentation Industries, 2016, 42(1):148-151.
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