研究报告

羟丙基甲基纤维素对冷冻麻薯生胚品质的改良效果及其机理研究

  • 张家萍 ,
  • 李文钊 ,
  • 余平莲 ,
  • 颜凤 ,
  • 杨晓飞 ,
  • 崔亚雪
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  • 1(天津科技大学 食品科学与工程学院,天津,300457)
    2(昭通学院 化学化工学院,云南省高校高原特色功能食品研究重点实验室,云南 昭通,657000)
第一作者:硕士研究生(李文钊教授和余平莲讲师为共同通信作者,E-mail:liwenzhao@tust.edu.cn;plyu@ztu.edu.cn)

收稿日期: 2024-08-04

  修回日期: 2024-09-06

  网络出版日期: 2025-07-11

基金资助

云南省教育厅科学研究基金项目(2024J1054)

Study on improvement effect and mechanism of hydroxypropyl methylcellulose on quality of frozen mochi dough

  • ZHANG Jiaping ,
  • LI Wenzhao ,
  • YU Pinglian ,
  • YAN Feng ,
  • YANG Xiaofei ,
  • CUI Yaxue
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  • 1(College of Food Science and Engineering, Tianjin University of Science & Technology, Tianjin 300457, China)
    2(Key Laboratory of Yunnan University for Plateau Characteristic Functional Food, School of Chemistry and Chemical Engineering, Zhaotong University, Zhaotong 657000, China)

Received date: 2024-08-04

  Revised date: 2024-09-06

  Online published: 2025-07-11

摘要

为进一步改善冷冻麻薯生胚焙烤产品的品质劣变问题,该文选取羟丙基甲基纤维素为改良剂,利用低场核磁共振仪、差式扫描量热仪、傅里叶变换红外光谱和扫描电子显微镜等技术检测冻融循环过程中冷冻麻薯生胚水分状态、蛋白质二级结构以及微观结构的变化,并通过食品体积测定仪、质构仪等仪器考察其烘焙品质的改良效果。结果表明,羟丙基甲基纤维素添加量可以改善麻薯比容,降低麻薯的硬度;4次冻融循环处理后,添加0.5%(质量分数)羟丙基甲基纤维素的麻薯比容高达3.71 cm3/g。同时,羟丙基甲基纤维素有效抑制了水分的迁移与流失,抑制冷冻麻薯生胚内部强结合水向弱结合和自由水的转换,提高了麻薯生胚的持水性;冷冻麻薯生胚的可冻结水含量随着羟丙基甲基纤维素的增加呈显著下降趋势。此外,冻融循环4次时,1.5%(质量分数)羟丙基甲基纤维素添加量下,冷冻麻薯生胚的游离巯基含量最低,α-螺旋含量最高;且蛋白质二级结构最为稳定,淀粉分子镶嵌到面筋蛋白网络结构中。因此,羟丙基甲基纤维素的添加改善了冷冻麻薯生胚的面团特性和烘焙品质,且0.5%(质量分数)羟丙基甲基纤维素添加量下烘焙品质改善效果最为明显。

本文引用格式

张家萍 , 李文钊 , 余平莲 , 颜凤 , 杨晓飞 , 崔亚雪 . 羟丙基甲基纤维素对冷冻麻薯生胚品质的改良效果及其机理研究[J]. 食品与发酵工业, 2025 , 51(12) : 226 -235 . DOI: 10.13995/j.cnki.11-1802/ts.040654

Abstract

To further improve the quality deterioration problems of the product after baking from frozen mochi dough, this article selected hydroxypropyl methylcellulose as the modifier, and used low-field nuclear magnetic resonance, differential scanning calorimetry, Fourier transform infrared spectroscopy, and scanning electron microscopy to detect the changes in moisture state, protein secondary structure, and microstructure of frozen mochi during freeze-thaw cycles, through food volume analyzer, texture analyzer, and other methods examined the improvement effects of baking quality.Results showed that hydroxypropyl methylcellulose improved the specific volume of mochi and reduced the hardness.The specific volume of mochi with 0.5% hydroxypropyl methylcellulose added was as high as 3.71 cm3/g after 4 freeze-thaw cycles.Meanwhile, hydroxypropyl methylcellulose effectively inhibited the migration and loss of water, prevented the conversion of strongly bound water to weakly bound and free water inside mochi dough, and increased the water-holding capacity of frozen mochi dough.The freezable water content of frozen mochi dough showed a significant decrease with the increase of hydroxypropyl methylcellulose.In addition, when the freeze-thaw cycle was 4 times, the free sulfhydryl content of frozen mochi dough was the lowest, and the a-helix content was the highest at 1.5%.The protein secondary structure was the most stable, and starch molecules were embedded in the gluten protein network structure.Therefore, the addition of hydroxypropyl methylcellulose improved the dough characteristics and baking quality of frozen mochi, and the baking quality improvement was most obvious at 0.5% addition.

参考文献

[1] 宋臻善, 周雪松, 曾建新.变性淀粉对麻糬品质的影响[J].现代食品科技, 2009, 25(10):1210-1211;1223.
SONG Z S, ZHOU X S, ZENG J X.Effect of modified starch on the properties of mochi[J].Modern Food Science and Technology, 2009, 25(10):1210-1211;1223.
[2] YU W J, XU D, ZHANG H, et al.Effect of pigskin gelatin on baking, structural and thermal properties of frozen dough:Comprehensive studies on alteration of gluten network[J].Food Hydrocolloids, 2020, 102:105591.
[3] LUO W H, SUN D W, ZHU Z W, et al.Improving freeze tolerance of yeast and dough properties for enhancing frozen dough quality—A review of effective methods[J].Trends in Food Science & Technology, 2018, 72:25-33.
[4] SHENG X L, MA Z, LI X P, et al.Effect of water migration on the thermal-vacuum packaged steamed buns under room temperature storage[J].Journal of Cereal Science, 2016, 72:117-123.
[5] LAGUNA L, PRIMO-MARTÍN C, VARELA P, et al.HPMC and inulin as fat replacers in biscuits:Sensory and instrumental evaluation[J].LWT - Food Science and Technology, 2014, 56(2):494-501.
[6] HAGER A S, ARENDT E K.Influence of hydroxypropylmethylcellulose (HPMC), xanthan gum and their combination on loaf specific volume, crumb hardness and crumb grain characteristics of gluten-free breads based on rice, maize, teff and buckwheat[J].Food Hydrocolloids, 2013, 32(1):195-203.
[7] BÁRCENAS M E, ROSELL C M.Effect of HPMC addition on the microstructure, quality and aging of wheat bread[J].Food Hydrocolloids, 2005, 19(6):1037-1043.
[8] XUAN Y F, ZHANG Y, ZHAO Y Y, et al.Effect of hydroxypropylmethylcellulose on transition of water status and physicochemical properties of wheat gluten upon frozen storage[J].Food Hydrocolloids, 2017, 63:35-42.
[9] 王静, 温纪平, 展小彬.羟丙基甲基纤维素对面团水分状态与油条品质的影响[J].粮食与油脂, 2022, 35(10):126-132.
WANG J, WEN J P, ZHAN X B.Effects of hydroxypropyl methylcellulose on moisture state of dough and quality of fried dough sticks[J].Cereals & Oils, 2022, 35(10):126-132.
[10] 黄齐林, 温纪平, 王静.羟丙基甲基纤维素对面团特性及油条品质的影响[J].食品科技, 2024, 49(5):132-139.
HUANG Q L, WEN J P, WANG J.Effect of hydroxypropyl methylcellulose on dough characteristics and fried dough sticks quality[J].Food Science and Technology, 2024, 49(5):132-139.
[11] 田霄翔, 李文钊, 冯亚倩, 等.两种乳化剂对冷冻麻薯坯及其烘烤品质的影响[J].食品研究与开发, 2023, 44(10):100-106.
TIAN X X, LI W Z, FENG Y Q, et al.Effect of two emulsifiers on frozen mochi dough and its baking quality[J].Food Research and Development, 2023, 44(10):100-106.
[12] 白妮. 冷冻生胚油条的品质劣变机理及改良研究[D].无锡:江南大学, 2022.
BAI N.Study on quality deterioration mechanism and improvement of frozen raw fried dough sticks[D].Wuxi:Jiangnan University, 2022.
[13] CHEN N, YANG Q, ZHANG C C, et al.Impact of basil seed gum on the textural, rheological properties, water state, gluten depolymerization and microstructure of frozen dough[J].Journal of Cereal Science, 2023, 112:103728.
[14] XIN C, NIE L J, CHEN H L, et al.Effect of degree of substitution of carboxymethyl cellulose sodium on the state of water, rheological and baking performance of frozen bread dough[J].Food Hydrocolloids, 2018, 80:8-14.
[15] XIAO F, ZHANG X, NIU M, et al.Gluten development and water distribution in bread dough influenced by bran components and glucose oxidase[J].LWT, 2021, 137:110427.
[16] 马珂莹. 面团冻藏过程中Biangbiang面条主要成分结构变化与品质稳定性关系研究[D].西安:陕西科技大学, 2022.
MA K Y.Study on the relationship between structural changes of main components and quality stability of Biangbiang noodles during frozen storage of dough[D].Xi’an:Shaanxi University of Science & Technology, 2022.
[17] 张淑芬, 吴宗帅, 岳双, 等.不同交联度木薯羟丙基淀粉在麻糬面包中的应用[J].食品科技, 2020, 45(8):215-220.
ZHANG S F, WU Z S, YUE S, et al.Application of tapioca hydroxypropylated starch with different cross-linking degree in preparation of mochi bread[J].Food Science and Technology, 2020, 45(8):215-220.
[18] 龚维, 吴磊燕, 钟雅云, 等.刺槐豆胶对冷冻面团水分分布及面包品质的影响[J].现代食品科技, 2018, 34(12):67-73.
GONG W, WU L Y, ZHONG Y Y, et al.Effect of locust bean gum on water distribution and bread quality of frozen dough[J].Modern Food Science and Technology, 2018, 34(12):67-73.
[19] LU L, YANG Z, GUO X N, et al.Effect of NaHCO3 and freeze-thaw cycles on frozen dough:From water state, gluten polymerization and microstructure[J].Food Chemistry, 2021, 358:129869.
[20] 金晓阳, 王秋玉, 唐振兴.麦芽糖醇对冷冻面团水分迁移及馒头食用品质的影响[J].食品与发酵工业, 2023, 49(10):146-154.
JIN X Y, WANG Q Y, TANG Z X.Effects of maltitol on water migration of frozen dough and edible quality of steamed bread[J].Food and Fermentation Industries, 2023, 49(10):146-154.
[21] GONG Y, DONG R, ZHANG K L, et al.The modulatory effect of guar gum on freeze-thaw stability of cooked oat roll[J].Food Hydrocolloids for Health, 2021, 1:100032.
[22] HE Y J, GUO J Y, REN G Y, et al.Effects of konjac glucomannan on the water distribution of frozen dough and corresponding steamed bread quality[J].Food Chemistry, 2020, 330:127243.
[23] ZHANG H, ZHANG Y Y, WANG X T, et al.Effects of bamboo shoot dietary fiber on mechanical properties, moisture distribution, and microstructure of frozen dough[J].Journal of Chemistry, 2017, 2017(1):4513410.
[24] 韩可阳, 刘亚伟, 刘洁.两种纤维素醚类亲水胶体对冷冻面团中面筋蛋白的影响[J].河南工业大学学报(自然科学版), 2022, 43(5):35-44.
HAN K Y, LIU Y W, LIU J.Effect of two hydrophilic cellulose ether on gluten in frozen dough[J].Journal of Henan University of Technology (Natural Science Edition), 2022, 43(5):35-44.
[25] 王宏伟, 国思琪, 苏会雨, 等.亚麻籽胶和沙蒿胶对冷冻面团及馒头品质的影响[J].轻工学报, 2023, 38(6):1-10.
WANG H W, GUO S Q, SU H Y, et al.Effects of flaxseed gum and Artemisia sphaerocephala Krasch.gum on the quality of frozen dough and steamed bread[J].Journal of Light Industry, 2023, 38(6):1-10.
[26] 刘雪梅, 郑卫平, 程颖, 等.羟丙基甲基纤维素对混合脂搅打奶油品质的影响[J].中国食品, 2023(24):150-153.
LIU X M, ZHENG W P, CHENG Y, et al.Effect of hydroxypropyl methylcellulose on the quality of mixed fat whipped cream[J].China Food, 2023(24):150-153.
[27] 宣以锋. 羟丙基甲基纤维素(HPMC)对冷冻面团加工性质的影响及相关机制研究[D].合肥:合肥工业大学, 2016.
XUAN Y F.Effect of hydroxypropyl methyl cellulose (HPMC) on processing properties of frozen dough and its related mechanism[D].Hefei:Hefei University of Technology, 2016.
[28] WANG P, CHEN H Y, MOHANAD B, et al.Effect of frozen storage on physico-chemistry of wheat gluten proteins:Studies on gluten-, glutenin- and gliadin-rich fractions[J].Food Hydrocolloids, 2014, 39:187-194.
[29] LIU J, LUO D L, LI X, et al.Effects of inulin on the structure and emulsifying properties of protein components in dough[J].Food Chemistry, 2016, 210:235-241.
[30] 郑子懿, 陆启玉, 章绍兵.面条冷冻过程中蛋白质组分和二硫键的变化研究[J].粮食与饲料工业, 2013(7):34-37.
ZHENG Z Y, LU Q Y, ZHANG S B.Research on change of protein and disulfide bond in noodles during frozen storage[J].Cereal & Feed Industry, 2013(7):34-37.
[31] 赵强忠, 杜翠, 蔡勇建, 等.HPMC和GOD对冷冻面团及其烘烤面包品质的影响[J].华南理工大学学报(自然科学版), 2019, 47(8):38-47.
ZHAO Q Z, DU C, CAI Y J, et al.Effects of HPMC and GOD on the quality of frozen dough and baked bread[J].Journal of South China University of Technology (Natural Science Edition), 2019, 47(8):38-47.
[32] 高杰,刘卫光,钟昔阳.羟丙基甲基纤维素对面团性质及油条品质的影响[J].农产品加工,2018(18):1-5.
GAO J, LIU W G, ZHONG X Y.Effect of hydroxypropyl methylcellulose on the properties of dough and the quality of youtiao[J].Farm Products Processing, 2018(18):1-5.
[33] ZHU X W, YUAN P P, ZHANG T, et al.Effect of carboxymethyl chitosan on the storage stability of frozen dough:State of water, protein structures and quality attributes[J].Food Research International, 2022, 151:110863.
[34] 王喜波, 曹佳, 杨赛, 等.大豆乳清蛋白与硫酸葡聚糖相互作用及乳化特性研究[J].农业机械学报, 2021, 52(10):409-416.
WANG X B, CAO J, YANG S, et al.Interaction and emulsification properties of soybean whey protein and dextran sulfate[J].Transactions of the Chinese Society for Agricultural Machinery, 2021, 52(10):409-416.
[35] 娄雪琪. 天然菊粉对冷冻面团及馒头品质的影响[D].洛阳:河南科技大学, 2022.
LOU X Q.Effect of natural inulin on the quality of frozen dough and steamed bread[D].Luoyang:Henan University of Science and Technology, 2022.
[36] ORTOLAN F, CORRÊA G P, DA CUNHA R L, et al.Rheological properties of vital wheat glutens with water or sodium chloride[J].LWT - Food Science and Technology, 2017, 79:647-654.
[37] 汪星星. 冻融冻藏过程中三种食品胶对面筋蛋白结构影响的研究[D].广州:华南农业大学, 2016.
WANG X X.Study on the influence of three kinds of food gums on gluten structure during freeze-thaw storage[D].Guangzhou:South China Agricultural University, 2016.
[38] 曹磊, 夏青, 宋玉, 等.发芽糙米和羟丙基甲基纤维素对面包品质的影响[J].食品工业科技, 2017, 38(14):55-60;70.
CAO L, XIA Q, SONG Y, et al.Effect of germinated brown rice and hydroxypropyl methyl cellulose on the quality of bread[J].Science and Technology of Food Industry, 2017, 38(14):55-60;70.
[39] PRIMO-MARTÍN C, SANZ T, STERINGA D W, et al.Performance of cellulose derivatives in deep-fried battered snacks:Oil barrier and crispy properties[J].Food Hydrocolloids, 2010, 24(8):702-708.
[40] MARIOTTI M, PAGANI M A, LUCISANO M.The role of buckwheat and HPMC on the breadmaking properties of some commercial gluten-free bread mixtures[J].Food Hydrocolloids, 2013, 30(1):393-400.
[41] MOREIRA R, CHENLO F, TORRES M D.Rheology of commercial chestnut flour doughs incorporated with gelling agents[J].Food Hydrocolloids, 2011, 25(5):1361-1371.
[42] LIU X L, MU T H, SUN H N, et al.Influence of different hydrocolloids on dough thermo-mechanical properties and in vitro starch digestibility of gluten-free steamed bread based on potato flour[J].Food Chemistry, 2018, 239:1064-1074.
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