该文以出芽短梗霉(Aureobasidium pullulans)CGMCCNO.7055作为出发菌株,利用GC-MS技术对不同通风条件下出芽短梗霉发酵产普鲁兰多糖的胞内代谢物情况进行分析,研究发现,通风对于普鲁兰多糖发酵有明显影响,在高通风(1∶1.4)下多糖产量最大为(80.2±1.4) g/L,通风比最低和最高之间,普鲁兰的产量相差13.50 g/L。对高低通风组发酵过程中代谢物进行分析。一共检测到了45种胞内小分子代谢物质,主要包括氨基酸类、有机酸类、糖类、醇类及一些其他类的代谢物质。将普鲁兰的代谢途径结合KEGG分析不同通风条件下菌体代谢的差异,差异代谢物主要涉及戊糖-葡萄糖醛酸转换途径、果糖-甘露糖代谢途径、柠檬酸循环途径和半乳糖代谢途径,最终导致普鲁兰多糖产量发生变化。为进一步了解出芽短埂霉产普鲁兰多糖的代谢机理研究提供参考。
In this paper, the intracellular metabolites of pullulan polysaccharide produced by Aureobasidium pullulans CGMCCNO.7055 were analyzed by GC-MS under different ventilation conditions using A.pullulans as the starting strain.It was found that ventilation had a significant effect on polysaccharide fermentation in pullulan, with the maximum polysaccharide yield of (80.2±1.4) g/L at high ventilation (1∶1.4) and a difference of 13.50 g/L between the lowest and the highest ventilation ratios in pullulan.The metabolites were analyzed in the fermentation of the high and low ventilation groups.A total of 45 intracellular small molecule metabolites were detected, mainly including amino acids, organic acids, sugars, alcohols, and some other classes of metabolites.The metabolic pathways of pullulan were combined with KEGG to analyze the differences in the metabolism of the fungus under different aeration conditions, and the differential metabolites were mainly related to the pentose-glucuronide conversion pathway, fructose-mannose metabolism pathway, citric acid cycling pathway, and galactose metabolism pathway, which ultimately led to changes in the production of pullulan polysaccharides.It provides a reference for further understanding of the metabolic mechanism study on the production of pullulan polysaccharides by germinating short ridge molds.
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