研究报告

基于饮食-肠道菌群-疾病相互作用数据库的膳食推荐对葡聚糖硫酸钠诱导的小鼠溃疡性结肠炎的缓解作用

  • 胡博 ,
  • 张惠琴 ,
  • 裴彰明 ,
  • 陈雪梅 ,
  • 唐晓姝 ,
  • 陆文伟
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  • 1(江南大学国家功能食品工程技术研究中心,江苏 无锡,214122)
    2(江南大学 食品学院,江苏 无锡,214122)
第一作者: 硕士,实验师(陆文伟研究员为通信作者,E-mail:luwenwei@jiangnan.edu.cn)

收稿日期: 2024-11-29

  修回日期: 2025-03-04

  网络出版日期: 2025-08-01

基金资助

国家自然科学基金面上项目(32172216)“十四五”国家重点研发子课题项目(2022YFF1100203)

Alleviating effect of dietary recommendations based on food-gut microbiota-disease interaction database on dextran sodium sulphate-induced ulcerative colitis in mice

  • HU Bo ,
  • ZHANG Huiqin ,
  • PEI Zhangming ,
  • CHEN Xuemei ,
  • TANG Xiaoshu ,
  • LU Wenwei
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  • 1(National Engineering Research Center for Functional Food, Jiangnan University, Wuxi 214122, China)
    2(School of Food Science and Technology, Jiangnan University, Wuxi 214122, China)

Received date: 2024-11-29

  Revised date: 2025-03-04

  Online published: 2025-08-01

摘要

膳食-肠道菌群-健康之间存在密切关联,开发肠道菌群靶向性食品以缓解疾病是一种切实可行的手段。该团队前期研究开发了一个饮食-肠道菌群-疾病相互作用(food-gut microbiota-disease interaction,FGMDI)数据库,其能根据疾病类型推荐膳食干预方案。该文以葡聚糖硫酸钠盐诱导的溃疡性结肠炎小鼠为实验对象,评估FGMDI数据库推荐的5种膳食干预方案对结肠炎的缓解作用。结果显示,模型对溃疡性结肠炎预测的27种肠道菌群靶点中有12种可在模型组与空白组的差异肠道菌群中发现,且其LDA score与推荐分数的方向和大小一致。以疾病活动性评分、结肠组织炎症及病理学、肠道屏障等作为评估指标,发现FGMDI推荐分数较高的MDCF、MSPrebiotic和绿色地中海干预3种膳食方案可以调控模型预测的肠道菌群靶点中的拟杆菌属、阿克曼氏菌属、大肠埃希氏菌、肠球菌属、罗氏菌属等肠道菌群。综上,该研究验证了FGMDI膳食推荐方案的有效性,为相关菌群靶向性食品开发提供了新思路。

本文引用格式

胡博 , 张惠琴 , 裴彰明 , 陈雪梅 , 唐晓姝 , 陆文伟 . 基于饮食-肠道菌群-疾病相互作用数据库的膳食推荐对葡聚糖硫酸钠诱导的小鼠溃疡性结肠炎的缓解作用[J]. 食品与发酵工业, 2025 , 51(14) : 17 -27 . DOI: 10.13995/j.cnki.11-1802/ts.041734

Abstract

There is a close correlation among diet, gut microbiota, and health.Developing gut microbiota-targeted foods to alleviate diseases is a practical approach.This team previously developed a food-gut microbiota-disease interaction (FGMDI) database, which could recommend dietary intervention plans according to different disease types.In this study, mice with dextran sulfate sodium-induced ulcerative colitis were used as experimental subjects to evaluate the alleviating effects of five dietary intervention plans recommended by the FGMDI database on colitis.Results showed that 12 out of the 27 gut microbiota targets predicted for ulcerative colitis by the model could be found among the differential gut microbiota between the model group and the blank group, and the direction and magnitude of their LDA scores were consistent with the recommended scores.Using disease activity scores, colon tissue inflammation, pathology, and intestinal barrier as evaluation indicators, it was found that three dietary plans, namely MDCF, MSPrebiotic, and the Green Mediterranean intervention, with higher recommended scores in FGMDI, could regulate gut microbiota such as Bacteroides, Akkermansia, Escherichia coli, Enterococcus, and Roseburia, which were the gut microbiota targets predicted by the model.In conclusion, this study verified the effectiveness of the dietary recommendation plans of FGMDI and provided new ideas for the development of related microbiota-targeted foods.

参考文献

[1] CUNNINGHAM A L, STEPHENS J W, HARRIS D A. A review on gut microbiota: A central factor in the pathophysiology of obesity[J]. Lipids in Health and Disease, 2021, 20(1):65.
[2] FAN Y, PEDERSEN O. Gut microbiota in human metabolic health and disease[J]. Nature Reviews Microbiology, 2020, 19(1):55-71.
[3] 朱春胜, 施亚敏, 付智慧.基于肠道菌群和代谢组学研究化滞柔肝颗粒治疗非酒精性脂肪肝的作用机制[J].中草药, 2023, 54(4):1190-1200.
ZHU C S, SHI Y M, FU Z H, et al. Mechanism of Huazhi Rougan Granule in treatment of non-alcoholic fatty liver disease based on intestinal flora and metabolomics [J]. Chinese Traditional and Herbal Drugs, 2023, 54(4): 1190-1200.
[4] PINTO S, BENINCÀ E, GALAZZO G, et al. Heterogeneous associations of gut microbiota with Crohn’s disease activity[J]. Gut Microbes, 2024, 16(1):2292239.
[5] HOLE M J, JØRGENSEN K K, HOLM K, et al. A shared mucosal gut microbiota signature in primary sclerosing cholangitis before and after liver transplantation[J]. Hepatology, 2023, 77(3):715-728.
[6] BARBERIO B, FACCHIN S, PATUZZI I, et al. A specific microbiota signature is associated to various degrees of ulcerative colitis as assessed by a machine learning approach[J]. Gut Microbes, 2022, 14(1):2028366.
[7] ARULSAMY A, TAN Q Y, BALASUBRAMANIAM V, et al. Gut microbiota and epilepsy: A systematic review on their relationship and possible therapeutics[J]. ACS Chemical Neuroscience, 2020, 11(21):3488-3498.
[8] LI Y X, ZHU W, JIANG Y, et al. Synthetic bacterial consortia transplantation for the treatment of Gardnerella vaginalis-induced bacterial vaginosis in mice[J]. Microbiome, 2023, 11(1):54.
[9] ZHAO Q X, YU J D, ZHOU H, et al. Intestinal dysbiosis exacerbates the pathogenesis of psoriasis-like phenotype through changes in fatty acid metabolism[J]. Signal Transduction and Targeted Therapy, 2023, 8:40.
[10] LAI H, LI Y F, HE Y F, et al. Effects of dietary fibers or probiotics on functional constipation symptoms and roles of gut microbiota: A double-blinded randomized placebo trial[J]. Gut Microbes, 2023, 15(1):2197837.
[11] ZENG X F, XING X, GUPTA M, et al. Gut bacterial nutrient preferences quantified in vivo[J]. Cell, 2022, 185(18):3441-3456.e19.
[12] LEEMING E R, JOHNSON A J, SPECTOR T D, et al. Effect of diet on the gut microbiota: Rethinking intervention duration[J]. Nutrients, 2019, 11(12):2862.
[13] PIAZZON M C, CALDUCH-GINER J A, FOUZ B, et al. Under control: How a dietary additive can restore the gut microbiome and proteomic profile, and improve disease resilience in a marine teleostean fish fed vegetable diets[J]. Microbiome, 2017, 5(1):164.
[14] ROY S, DHANESHWAR S. Role of prebiotics, probiotics, and synbiotics in management of inflammatory bowel disease: Current perspectives[J]. World Journal of Gastroenterology, 2023, 29(14):2078-2100.
[15] PELED S, LIVNEY Y D. The role of dietary proteins and carbohydrates in gut microbiome composition and activity: A review[J]. Food Hydrocolloids, 2021, 120:106911.
[16] ZHANG H Q, ZHANG J, ZHAO L, et al. Comprehensive database for food-gut microbiota-disease interactions (FGMDI) analysis and dietary recommendation applications[J]. Food Bioscience, 2024, 60:104091.
[17] GU W C, ZHANG L K, HAN T, et al. Dynamic changes in gut microbiome of ulcerative colitis: Initial study from animal model[J]. Journal of Inflammation Research, 2022, 15:2631-2647.
[18] WANG F, CHEN Y R, ITAGAKI K, et al. Wheat germ-derived peptide alleviates dextran sulfate sodium-induced colitis in mice[J]. Journal of Agricultural and Food Chemistry, 2023, 71(42):15593-15603.
[19] GEHRIG J L, VENKATESH S, CHANG H W, et al. Effects of microbiota-directed foods in gnotobiotic animals and undernourished children[J]. Science, 2019, 365(6449): eaau4732.
[20] KAPLAN A, ZELICHA H, MEIR A Y, et al. The effect of a high-polyphenol Mediterranean diet (Green-MED) combined with physical activity on age-related brain atrophy: The Dietary Intervention Randomized Controlled Trial Polyphenols Unprocessed Study (DIRECT PLUS)[J]. The American Journal of Clinical Nutrition, 2022, 115(5):1270-1281.
[21] QIN W, LUO H, YANG L, et al. Rubia cordifolia L. ameliorates DSS-induced ulcerative colitis in mice through dual inhibition of NLRP3 inflammasome and IL-6/JAK2/STAT3 pathways[J]. Heliyon, 2022, 8(8): e10314.
[22] 刘慧敏, 侯慧, 李丹萍. 高摄入红肉通过改变肠道菌群加重小鼠溃疡性结肠炎[J]. 中国微生态学杂志, 2023, 35(4):396-402.
LIU H M, HOU H, LI D P. High red meat intake exacerbates ulcerative colitis in mice by altering gut microbiota[J]. Chinese Journal of Microecology, 2023, 35(4):396-402.
[23] HUANG X L, XU J, ZHANG X H, et al. PI3K/Akt signaling pathway is involved in the pathogenesis of ulcerative colitis[J]. Inflammation Research, 2011, 60(8):727-734.
[24] WANG C H, PAN Z H, JIN Y X. F-53B induces hepatotoxic effects and slows self-healing in ulcerative colitis in mice[J]. Environmental Pollution, 2023, 317:120819.
[25] NARIYA M, NARIYA PANKAJ, RAVISHANKAR B, et al. Ameliorative effects of Triphala on mucosal damage in rat model of ulcerative colitis[J]. Indian Journal of Traditional Knowledge, 2021, 20(4): 951-955.
[26] OZ H S, ZHONG J, DE VILLIERS W J S. Osteopontin ablation attenuates progression of colitis in TNBS model[J]. Digestive Diseases and Sciences, 2012, 57(6):1554-1561.
[27] MARINO M, RENDINE M, VENTURI S, et al. Red raspberry (Rubus idaeus) preserves intestinal barrier integrity and reduces oxidative stress in Caco-2 cells exposed to a proinflammatory stimulus[J]. Food & Function, 2024, 15(13):6943-6954.
[28] TONG L J, ZHANG X Y, HAO H N, et al. Lactobacillus rhamnosus GG derived extracellular vesicles modulate gut microbiota and attenuate inflammatory in DSS-induced colitis mice[J]. Nutrients, 2021, 13(10):3319.
[29] LI W Y, ZHANG Y, CHEN M Y, et al. The antioxidant strain Lactiplantibacillus plantarum AS21 and Clostridium butyricum ameliorate DSS-induced colitis in mice by remodeling the assembly of intestinal microbiota and improving gut functions[J]. Food & Function, 2024, 15(4):2022-2037.
[30] CHATTERJEE A, KUMAR S, ROY SARKAR S, et al. Dietary polyphenols represent a phytotherapeutic alternative for gut dysbiosis associated neurodegeneration: A systematic review[J]. The Journal of Nutritional Biochemistry, 2024, 129:109622.
[31] MARIAT D, FIRMESSE O, LEVENEZ F, et al. The Firmicutes/Bacteroidetes ratio of the human microbiota changes with age[J]. BMC Microbiology, 2009, 9:123.
[32] YANG J P, KURNIA P, HENNING S M, et al. Effect of standardized grape powder consumption on the gut microbiome of healthy subjects: A pilot study[J]. Nutrients, 2021, 13(11):3965.
[33] MILLS R H, DULAI P S, VÁZQUEZ-BAEZA Y, et al. Multi-omics analyses of the ulcerative colitis gut microbiome link Bacteroides vulgatus proteases with disease severity[J]. Nature Microbiology, 2022, 7(2):262-276.
[34] STINEMETZ E K, GAO P, PINKSTON K L, et al. Processing of the major autolysin of E. faecalis, AtlA, by the zinc-metalloprotease, GelE, impacts AtlA septal localization and cell separation[J]. PLoS One, 2017, 12(10): e0186706.
[35] ICHIMIYA T, KAZAMA T, ISHIGAMI K, et al. Application of plasma alternative to serum for measuring leucine-rich α2-glycoprotein as a biomarker of inflammatory bowel disease[J]. PLoS One, 2023, 18(6): e0286415.
[36] KARPIŃSKI T M, OŻAROWSKI M, STASIEWICZ M. Carcinogenic microbiota and its role in colorectal cancer development[J]. Seminars in Cancer Biology, 2022, 86:420-430.
[37] ZHOU Z, YANG W J, YU T M, et al. GPR120 promotes neutrophil control of intestinal bacterial infection[J]. Gut Microbes, 2023, 15(1):2190311.
[38] GU Z P, XU S Q, ZHENG Y X, et al. In vitro faecal fermentation metabolites of 2’-fucosyllactose protect against intestinal epithelial injury: Infant enterotype effects[J]. International Dairy Journal, 2024, 150:105860.
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