食品与发酵工业 >

2025 , Vol. 51 >Issue 14: 89 - 96

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.042382

研究报告

沙棘油对大鼠酒精性肝损伤的辅助保护作用研究

  • 韦琦劼 ,
  • 王靓 ,
  • 汪小雅 ,
  • 吴文婧 ,
  • 倪梦梅 ,
  • 陈锦瑶
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  • (四川大学 华西公共卫生学院,华西第四医院,四川 成都,610041)
第一作者: 硕士研究生(陈锦瑶教授为通信作者,E-mail:umbrellayy@163.com)

收稿日期: 2025-02-16

  修回日期: 2025-03-11

  网络出版日期: 2025-08-01

基金资助

863计划重点项目(2010AA023001)

收起

Study on auxiliary protective effect of seabuckthorn oil on alcoholic liver injury in rats

  • WEI Qijie ,
  • WANG Liang ,
  • WANG Xiaoya ,
  • WU Wenjing ,
  • NI Mengmei ,
  • CHEN Jinyao
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  • (West China School of Public Health, West China Fourth Hospital, Sichuan University, Chengdu 610041, China)

Received date: 2025-02-16

  Revised date: 2025-03-11

  Online published: 2025-08-01

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摘要

该研究旨在探讨沙棘油对大鼠酒精性肝损伤的辅助保护作用。将100只雄性SD大鼠随机分为空白对照组、模型组和沙棘油低、中、高剂量组,通过50%(体积分数)无水乙醇诱导建立急性肝损伤动物模型,分析比较不同剂量沙棘油受试物对大鼠肝脏代谢酶类、肝脏及血清脂质水平及肝脏脂质蓄积及酒精代谢酶的影响。结果表明,各组大鼠体重及增重、肝脏质量、肝脏指数均无显著性差异;给予受试物30 d,与模型组相比,各剂量组的肝脏还原型谷胱甘肽水平均显著升高,而肝脏甘油三酯、血清总胆固醇和丙氨酸氨基转移酶水平显著降低,且受试物低、中剂量组的肝脏丙二醛水平显著降低,高剂量组的血清天门冬氨酸氨基转移酶和低密度脂蛋白胆固醇水平显著降低;给予受试物50 d,低剂量组丙二醛水平和病理评分与模型组相比显著降低,还原型谷胱甘肽水平显著增高,中、高剂量组丙二醛水平明显下降,各剂量组肝脏乙醛脱氢酶及乙醇脱氢酶活力无显著差异。结果表明,沙棘油可能通过抗氧化作用和调节脂质代谢减少肝细胞损伤,从而表现出对酒精性肝损伤具有辅助保护作用,但沙棘油对肝脏生化指标和血清生化指标的影响呈现复杂的剂量依赖性关系,在实际应用中,应根据具体情况选择合适的沙棘油剂量。该研究为沙棘油在食品、保健食品等领域的应用提供了科学依据。

关键词： 沙棘油; 抗氧化; 大鼠; 酒精性肝损伤; 脂质蓄积; 酒精代谢酶

本文引用格式

韦琦劼 , 王靓 , 汪小雅 , 吴文婧 , 倪梦梅 , 陈锦瑶 . 沙棘油对大鼠酒精性肝损伤的辅助保护作用研究[J]. 食品与发酵工业, 2025 , 51(14) : 89 -96 . DOI: 10.13995/j.cnki.11-1802/ts.042382

Abstract

The purpose of this study was to investigate the auxiliary protective effect of seabuckthorn oil on alcoholic liver injury in rats.In the experiment, 100 male SD rats were randomly divided into blank control group, model control group and low, medium and high dose groups of seabuckthorn oil.The animal model of acute liver injury was induced by 50% anhydrous ethanol.The effects of different doses of seabuckthorn oil on liver metabolic enzymes, liver and serum lipid levels, liver lipid accumulation and alcohol metabolic enzymes in rats were analyzed and compared.Results showed that there was no significant difference in body weight and weight gain, liver weight and liver index among the groups.After 30 days of administration of the test substance, compared with the model control group, the levels of reduced glutathione in the liver of each dose group were significantly increased, while the levels of triglyceride in the liver, serum total cholesterol and alanine aminotransferase were significantly decreased, the levels of malondialdehyde (MDA) in the liver of the low and medium dose groups were significantly decreased, and the serum levels of aspartate aminotransferase and low-density lipoprotein cholesterol were significantly reduced in the high dose group.After giving the test substance for 50 days, the MDA level and pathological score of the low dose group were significantly lower than those of the model control group, the level of reduced glutathione was significantly increased, and the MDA level of the middle and high dose groups was significantly decreased.There was no significant difference in the activity of acetaldehyde dehydrogenase and alcohol dehydrogenase in the liver of each dose group of 50 days.The results showed that seabuckthorn oil may reduce liver cell damage through antioxidant effects and regulation of lipid metabolism, thereby providing auxiliary protection against alcoholic liver injury.However, the effects of seabuckthorn oil on liver biochemical indicators and serum biochemical indicators exhibited a complex dose-dependent relationship.In practical applications, the appropriate dosage of seabuckthorn oil should be selected according to specific circumstances.This study provides a scientific basis for the application of seabuckthorn oil in food products, nutraceuticals, and related industries.

Key words： seabuckthorn oil; antioxidant; rat; alcoholic liver injury; lipid accumulation; alcohol metabolic enzymes

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